Comparison of histologic stains for use in PCR analysis of microdissected, paraffin-embedded tissues

BioTechniques

Volumn 24, Issue 1, 1998, Pages 86-92

  Burton, M P ^a   Schneider, B G ^d   Brown, R ^b   Escamilla Ponce, N ^b   Gulley, Margaret L ^b,c  

^a WILFORD HALL MEDICAL CENTER   (United States)

^b UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^c SOUTH TEXAS VETERANS HEALTH CARE SYSTEM   (United States)

^d LOUISIANA STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; EVANS BLUE; HEMATOXYLIN; METHYL GREEN; NEUTRAL RED; SILVER;

ARTICLE; CARCINOMA; CHROMATIN; CONTROLLED STUDY; DISSECTION; DNA TEMPLATE; ELECTROPHORESIS; HUMAN; HUMAN TISSUE; MICROSCOPY; POLYMERASE CHAIN REACTION; STAINING; TISSUE;

EID: 0031912481     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98241st01     Document Type: Article

References (8)

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2. Bianchi, A.B., N.M. Navone and C.J. Conti. 1991. Detection of loss of heterozygosity in formalin-fixed paraffin-embedded tumor specimens by the polymerase chain reaction. Am. J. Pathol. 138:279-284.
3. Greer, C.E., J.K. Lund and M. Manos. 1991. PCR amplification from paraffin-embedded tissues: recommendations on fixatives for long-term storage and prospective studies. PCR Methods Appl. 1:46-50.
4. Greer, C.E., S.L. Peterson, N.B. Kiviat and M. Manos. 1991. PCR amplification from paraffin-embedded tissues. Am. J. Clin. Pathol. 95:117-124.
5. Schneider, B.G., D.R. Pulitzer, R.D. Brown, T.J. Prihoda, D.G. Bostwick, V. Saldivar, H.A. Rodriguez-Martinez, M.E. Gutierrez-Diaz C. and P. O'Connell. 1995. Allelic imbalance in gastric cancer: an affected site on chromosome 3p. Genes Chrom. Cancer 13:263-271.
6. Sheehan, D.C. and B.B. Hrapchak. 1980. Nuclear and cytoplasmic staining, p. 137-158. In D.C. Sheehan and B.B Hrapchak (Eds.), Theory and Practice of Histotechnology, 2nd ed. CV Mosby Co., St. Louis.
7. Shibata, D. 1992. The polymerase chain reaction and the molecular genetic analysis of tissue biopsies, p. 85-111. In C.S. Herrington and J.O.D. McGee (Eds.), Diagnostic Molecular Pathology: A Practical Approach, Vol II. IRL Press, Oxford, England.
8. Stevens, A. 1990. The hematoxylins, p. 107-118. In J.D. Bancroft and A. Stevens (Eds.), Theory and Practice of Histological Techniques, 3rd ed. Churchill Livingstone, Edinburgh.