Polycyclic aromatic hydrocarbons as inducers of cytochrome P4501A enzyme activity in the rainbow trout liver cell line, RTL-W1 and in primary cultures of rainbow trout hepatocytes

Environmental Toxicology and Chemistry

Volumn 20, Issue 3, 2001, Pages 632-643

  Behrens, Anja ^a   Schirmer, Kristin ^b   Bols, Niels C ^b   Segner, Helmut ^a  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^b UNIVERSITY OF WATERLOO   (Canada)

Author keywords

7 Ethoxyresorufin O deethylase; Intact primary hepatocytes; Polycyclic aromatic hydrocarbons; Rainbow trout; RTL W1 cell line

Indexed keywords

AROMATIC HYDROCARBONS; CATALYSIS; CELL CULTURE; CELLS; ENZYMES;

CYTOCHROME;

TOXICITY;

3 METHYLCHOLANTHRENE; ANTHRACENE; ANTHRACENE DERIVATIVE; BENZO[A]PYRENE; CHRYSENE; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; ETHOXYRESORUFIN DEETHYLASE; FLUORANTHENE; PHENANTHRENE; POLYCYCLIC AROMATIC HYDROCARBON; PYRENE;

PAH; PHYSIOLOGICAL RESPONSE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CATALYSIS; CELL LINE; CELL SPECIFICITY; CONCENTRATION (PARAMETERS); CULTURE MEDIUM; ENVIRONMENTAL EXPOSURE; ENZYME INDUCTION; LIVER; LIVER CELL; NONHUMAN; PRIORITY JOURNAL; RAINBOW TROUT;

ONCORHYNCHUS MYKISS;

EID: 0034833427     PISSN: 07307268     EISSN: None     Source Type: Journal    
DOI: 10.1002/etc.5620200324     Document Type: Article

References (38)

1. Ability of polycyclic aromatic hydrocarbons to induce 7-ethoxyresorufin-O-deethylase activity in a trout liver cell line
2. Comparison of two bioassays, a fish liver cell line (PLHC- 1) and a midge (Chironomus riparius), in monitoring freshwater sediments
3. Characterization of the H4IIE rat hepatoma cell bioassay for evaluation of environmental samples containing polynuclear aromatic hydrocarbons (PAHs)
4. Development of a cell-line from primary cultures of rainbow-trout, Oncorhynchus mykiss (Walbaum), gills
5. Fish cell lines as a tool in aquatic toxicology
6. Characterization of two continuous cell lines derived from Oncorhynchus myikss for models of arylhydrocarbon-receptor-mediated signal transduction. Direct comparison to the mammalian HEPA-lclc7 cell line
7. Characterization and induction of xenobiotics metabolizing enzyme activities in a primary culture of rainbow trout hepatocytes
8. Expression of P4501A1 in a primary culture of rainbow trout hepatocytes exposed to β-naphthoflavone or 2,3,7,8-tetrachlorodibenzo-p-dioxin
9. Review: Isolation and primary culture of teleost hepatocytes
10. Development and characterization of a rainbow trout liver cell line expressing cytochrome P450-dependent monooxygenase activity
11. Viability and differential function of rainbow trout liver cells in primary culture: Coculture with two permanent fish cells
12. Comparative in vitro and in vivo benzo[α]pyrene-DNA adduct formation and its relationship to CYP1A activity in two species of ictalurid catfish
13. Cytochrome P450IA1 in hepatic lesions of a teleost fish (Fundulus heteroclitus) collected from a polycyclic aromatic hydrocarbon-contaminated site
14. Potency of various polycyclic aromatic hydrocarbons as inducers of CYPIA1 in rat hepatocyte cultures
15. Identification of cytochrome P4501A inducers in complex mixtures of polycyclic aromatic hydrocarbon
16. Metabolism of benzo[α]pyrene in fish hepatocytes cultured on microplates
17. Metabolism and DNA adduct formation of benzo[α]pyrene and 7,12-dimethylbenz[α]anthracene in fish cell lines and culture
18. 2,3,7,8-Tetrachlorodibenzo-p- dioxin versus 3-methylcholanthrene: Comparative studies of Ah receptor binding, transformation, and induction of CYP1A1
19. Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes
20. Water-related environmental fate of 129 priority pollutants. EPA-440/4-79-02
21. Microassay for rapid measurement of 7-ethoxyresorufin-O-deethylase activity in intact fish hepatocytes
22. A fluorescence-based protein assay for use with a microplate reader
23. Two unique CYP1 genes are expressed in response to 3-methylcholanthrene treatment in rainbow trout
24. Hepatic mixed-function oxidase activity in rainbow trout exposed to several polycyclic aromatic compounds
25. Induction of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) liver cells: Concentration-response relationships of four model substances
26. Temporal induction of cytochrome P4501A in the mirror carp (Cyprinus carpio) after administration of several polycyclic aromatic hydrocarbons
27. Assessment of the bioavailability of PAHs in rats exposed to a polluted soil by natural routes: Induction of EROD activity and DNA adducts and PAH burden in both liver and lung
28. Cytochrome P4501A induction and inhibition by 3,3′,4,4′tetrachlorobiphenyl in an Ah receptor containing fish hepatoma cell line (PLHC-1)
29. Isolation and cultivation of teleost hepatocytes
30. Comparison of rat hepatocyte and differentiated hepatoma cell line culture as bioindicators of CYP1A1 inducers in urban air
31. Derivation of 2,3,7,8-TCDD toxic equivalent factors (TEFs) for selected dioxins, furans and PCBs with rainbow trout and rat liver cell lines and the influence of exposure time
32. The cytosolic receptor binding affinities and AHH induction potencies of 29 polynuclear aromatic hydrocarbons
33. Methodology for demonstrating and measuring the phototoxicity of fluoranthene to fish cells in culture
34. Lipoproteins in cellular uptake of benzo[α]pyrene
35. Toxic equivalency factors (TEFs) for polycyclic aromatic hydrocarbons (PAHs)
36. Induction of EROD activity and genotoxicity effects by polycyclic aromatic hydrocarbons and resin acids on the juvenile eel (Anguilla anguilla L.)
37. Fixed-effectlevel toxic equivalentsA suitable parameter for asessing EROD induction potency in complex environmental samples
38. A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions