Development of a monoclonal antibody for ELISA of CYP1A in primary cultures of rainbow trout (Oncorhynchus mykiss) hepatocytes

Biomarkers

Volumn 2, Issue 5, 1997, Pages 287-294

  Scholz, S ^a,e   Behn, I ^b   Honeck, H ^c   Hauck, C ^d   Braunbeck, T ^d   Segner, H ^a  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^b UNIVERSITY OF LEIPZIG   (Germany)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^d UNIVERSITY OF HEIDELBERG   (Germany)

^e DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

Cytochrome P4501A; ELISA; Induction; Monoclonal antibody; Primary culture

Indexed keywords

EID: 0000109848     PISSN: 1354750X     EISSN: None     Source Type: Journal    
DOI: 10.1080/135475097231517     Document Type: Article

References (38)

1. ACHAZI, R. K., CHROSZCZ, G., HEIMIG, E., NEUNABER, R., AND STEUDEL, I. (1994) A high-molecular regulator of 7-ethoxyresorufin-O-deethylase activity in fish. Comparative Biochemistry and Physiology C, 108, 243-256.
2. BEHN, I. AND FIEBIG, H. (1984) Herstellung monoklonaler Antikörper durch Lymphozytenhybridomtechnik. Wissenschaftliche Zeitschrift Karl-Marx-Universität Leipzig, Mathematisch-Naturwissenschaftliche Reihe, 33, 612-618.
3. BLAIR, J. B., MILLER, R. M., PACK, D., BARNES, R., TEH, S. J. AND HINTON, D. E. (1990) Isolated trout liver cells: establishing short-term primary cultures exhibiting cell-to-cell interactions. In Vitro Cellular & Developmental Biology, 26, 237-249.
4. BOCK, K. W. (1993) Aryl hydrocarbon ordioxin receptor: biologic and toxic responses. In Reviews of Physiology, Biochemistry and Pharmacology, Volume 125, M. P. Blaustein, H. Grunicke, E. Habermann, D. Pette, H. Reuter, B. Sakmann, M. Schweiger, E. R. Weibel and E. M. Wright, eds (Springer-Verlag, Berlin), pp. 1-42.
5. BOON, J. P., EVERAATS, J. M., HILLEBRAND, M. T. J., EGGENS, M. L., PIJNENBURG, J. AND GOKSØYR, A. (1992) Changes in levels of hepatic biotransformation enzymes and haemoglobin levels in female plaice (Pleuronectes platessa) after oral administration of a technical polychlorinated biphenyl mixture (Clophen A40). Science of the Total Environment, 114, 113-133.
6. BRADFORD, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Analytical Biochemistry, 72, 248-254.
7. BRAUNBECK, T. AND STORCH, V. (1993) Senesescence of hepatocytes isolated from rainbow trout (Oncorhynchus mykiss) in primary culture: an ultrastructural study in primary hepatocytes. Protoplasma, 170, 135-150.
8. BRAUNBECK, T. AND VOELKL, A. (1991) Induction of biotransformation in the liver of eel (Anguilla anguilla L.) by sublethal exposure to dinitro-o-cresol: an ultrastructural and biochemical study. Ecotoxicology and Environmental Safety, 21, 109-27.
9. BRUSCHWEILER, B. J., WÜRGLER, F. E. AND FENT, K. (1996) An ELISA assay for cytochrome P4501A in fish liver cells: Environmental Toxicology and Chemistry, 15, 592-596.
10. BURKE, M. D. AND MAYER, R. T. (1974) Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metabolism and Disposition, 2, 583-588.
11. CELANDER, M. AND FÖRLIN, L. (1991) Catalytic activities and immunochemical quantification of hepatic cytochrome P450 in β-naphthoflavone treated rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 9, 189-197.
12. CLEMONS, J. H., VAN DEN HEUVEL, M. R., STEGEMAN, J. J., DIXON, D. G. AND BOLS, N. C. (1994) Comparison of toxic equivalent factors for selected dioxin and furan congeners derived using fish and mammalian liver cell lines. Canadian Journal of Fisheries and Aquatic Sciences, 51, 1577-1584.
13. FÖRLIN, L. AND CELANDER, M. (1993) Induction of cytochrome P4501A in teleosts: Environmental monitoring and Swedish fresh, brackish and marine waters. Aquatic Toxicology, 26, 41-56.
14. GOKSØYR, A. (1991) A semi-quantitative cytochrome P450IA1 ELISA: a simple method for studying the monooxygenase induction response in environmental monitoring and ecotoxicological testing of fish. Science of the Total Environment, 101, 255-262.
15. GOKSØYR, A. AND FÖRLIN, L. (1992) The cytochrome P-450 system in fish, aquatic toxicology and environmental monitoring. Aquatic Toxicology, 22, 287-311.
16. GOKSØYR, A., ANDERSSON, T., HANSSON, T., KLUNGSOYR, J., ZHANG, Y. AND FÖRLIN, L. (1987) Species characteristics of the hepatic xenobiotic and steroid biotransformation systems of two teleost fish, Atlantic cod (Gadus morhua) and rainbow trout (Salmo gairdneri). Toxicology and Applied Pharmacology, 89, 347-360.
17. GOKSØYR, A., ANDERSSON, T., BUHLER, D. R., STEGEMAN, J. J., WILLIAMS, D. E. AND FÖRLIN, L. (1991a) Immunochemical cross-reactivity of β-naphtoflavone-inducible cytochrome P450 (P4501A) in liver microsomes from different fish species and rat. Fish Physiology and Biochemistry, 9, 1-13.
18. GOKSØYR, A., LARSEN, H. E. AND HUSOY, A. M. (1991b) Application of a cytochrome P-4501A1-ELISA in environmental monitoring and toxicological testing of fish. Comparative Biochemistry and Physiology C, 100, 157-160.
19. GOLDSTEIN, J. A. AND FALETTO, M. B. (1993) Advances in mechanism of activation and deactivation of environmental chemicals. Environmental Health Perspectives, 100, 169-176.
20. GOOCH, J. W., ELSKUS, A. A., KLOEPPER-SAMS, P. J., HAHN, M. E. AND STEGEMAN, J. J. (1989) Effects of ortho- and non-ortho-substituted polychlorinated biphenyl congeners on the hepatic monooxygenase system in scup (Stenothomus chrysops). Toxicology and Applied Pharmacology, 98, 422-433.
21. HAASCH, M. L., WEJKSNORA, P. J., STEGEMAN, J. J. AND LECH, J. J. (1989) Cloned rainbow trout liver P(1)450 complementary DNA as a potential environmental monitor. Toxicology and Applied Pharmacology, 98, 362-368.
22. HAHN, M. E., WOODWARD, A. R., STEGEMAN, J. J. AND KENNEDY, S. W. (1996) Rapid assessment of induced cytochrome P4501A protein and catalytic activity in fish hepatoma cells grown in multiwell plates: response to TCDD, TCDF and two planar PCBs. Environmental Toxicology and Chemistry, 15, 582-592.
23. HEILMANN, L. J., SHEEN Y. Y., BIGELOW, S. W. AND NEBERT, D. W. (1988) Trout P450IA1: cDNA and deduced protein sequence, expression in liver, and evolutionary significance. DNA, 7, 379-387.
24. IOANNIDES, C. AND PARKE, D. V. (1993) Induction of cytochrome P4501 as an indicator of potential chemical carcinogenesis. Drug Metabolism Reviews, 25, 485-501.
25. KÖHLER, G. AND MILSTEIN, C. (1975) Continuous cultures of fused cells secreting antibodies of predefined specificity. Nature, 256, 495-497.
26. LECH, J. J. AND BEND, J. R. (1980) Relationship between biotransformation and the toxicity and fate of xenobiotic chemicals in fish. Environmental Health Perspectives, 34, 115-131.
27. MILLER, M. R., SAITO, N., BLAIR, J. B. AND HINTON, D. E. (1993a) Acetaminophen toxicity in cultured trout liver cells. II. Maintenance of cytochrome P450 1A1. Experimental and Molecular Pathology, 58, 114-126.
28. MILLER, M. R., WENTZ, E., BLAIR, J. B., PACK, D. AND HINTON, D. E. (1993b) Acetaminophen toxicity in cultured trout liver cells. I. Morphological alterations and effects on cytochrome P450 1A1. Experimental and Molecular Pathology, 58, 127-138.
29. OMURA, T. AND SATO, R. (1964) The carbon monoxide-binding pigment of liver microsomes - I. Evidence for its hemoprotein nature. Journal of Biological Chemistry, 239, 2370-2377.
30. PESONEN, M., GOKSØYR, A. AND ANDERSSON, T. (1992) Expression of P4501A1 in a primary culture of rainbow trout hepatocytes exposed to β-naphthoflavone or 2,3,7,8-tetrachlorodibenzo-dioxin. Archives of Biochemistry and Biophysics, 292, 228-232.
31. PETERS, J. H. AND BAUMGARTEN, M. (1988) Monoklonale Antikörper: Herstellung und Charakterisierung (Springer-Verlag, Berlin).
32. PLUTA, H. J. (1992) Enzymatic procedures for detection of biotransformation (detoxication activity) of the fish liver - a contribution to monitoring biological effects. Schriftenreihe zu Verfahren in Wasser, Boden und Lufthygiene, 89, 821-830.
33. SEGNER, H. (1997) Isolation and primary culture of teleost hepatocytes. Comparative Biochemistry and Physiology B (in press).
34. SIJM, D. T. H. M., SCHAAP, G. AND OPPERHUIZEN, A. (1993a) The effect of the biotransformation inhibitor piperonylbutoxide on the bioconcentration of 2,8-dichlrodibenzo-p-dioxin and pentachlorobenzene in goldfish. Aquatic Toxicology (Amsterdam), 27, 345-360.
35. STEGEMAN, J. J., KLOEPPER-SAMS, P. J. AND FARRINGTON, J. W. (1986) Monooxygenase induction and chlorobiphenyls in the deep-sea fish (Coryphaenoides armatus). Science, 231, 1287-1289.
36. ULLRICH, V. AND WEBER, P. (1972) The O-dealkylation of 7-ethoxycoumarin by liver microsomes - a direct fluorometric test. Hoppe Seyler's Zeitschrift für Physiologische Chemie, 353, 1171-1177.
37. VAN DER WEIDEN, M. E. J., TIBOSCH, H. J. H., BLEUMINK, R., SINNIGE, T. L., VAN DE GUCHTEN, C., SEINEN, W. AND VAN DER BERG, M. (1993) Cytochrome P450 IA induction in the common carp (Cyprinus carpio) following exposure to contaminated sediments with halogenated polyaromatics. Chemosphere, 27, 1297-1309.
38. ZHANG, Y. S., ANDERSSON, T. AND FÖRLIN, L. (1990) Induction of hepatic xenobiotic biotransformation enzymes in rainbow trout by beta-naphthoflavone. Time-course studies. Comparative Biochemistry and Physiology B, 95, 247-253.