Positive selection of mutations leading to loss or reduction of transcriptional activity of PrfA, the central regulator of Listeria monocytogenes virulence

Journal of Bacteriology

Volumn 183, Issue 19, 2001, Pages 5562-5570

  Herler, M ^a   Bubert, A ^a   Goetz, M ^a   Vega, Y ^a   Vazquez Boland, J A ^a   Goebel, W ^a  

^a UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENE PRODUCT; HELIX LOOP HELIX PROTEIN; PROTEIN IAP; PROTEIN P60; RNA POLYMERASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR PRFA; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BINDING SITE; GENE CONTROL; GENE EXPRESSION; GENE FUNCTION; GENE MUTATION; GENE OVEREXPRESSION; GENE STRUCTURE; GENETIC TRANSCRIPTION; LISTERIA MONOCYTOGENES; NONHUMAN; PATHOGENESIS; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN MOTIF; SEQUENCE ANALYSIS; STRAIN DIFFERENCE; VIRULENCE;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BACTERIAL TOXINS; GENE EXPRESSION REGULATION, BACTERIAL; HEAT-SHOCK PROTEINS; HEMOLYSIN PROTEINS; LISTERIA MONOCYTOGENES; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE TERMINATION FACTORS; PLASMIDS; PROMOTER REGIONS (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION, GENETIC; TRANSFORMATION, BACTERIAL; VIRULENCE;

BACILLUS SUBTILIS; LISTERIA MONOCYTOGENES; POSIBACTERIA;

EID: 0034829767     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.19.5562-5570.2001     Document Type: Article

References (57)

1. PrfA mediates specific binding of RNA polymerase of Listeria monocytogenes to PrfA-dependent virulence gene promoters resulting in a transcriptionally active complex
2. Specific binding of the Listeria monocytogenes transcriptional regulator PrfA to target sequences requires additional factor(s) and is influenced by iron
3. Differential regulation of the virulence genes of Listeria monocytogenes by the transcriptional activator PrfA
4. Differential expression of Listeria monocytogenes virulence genes in mammalian host cells
5. The Listeria monocytogenes iap gene as an indicator gene for the study of PrfA-dependent regulation
6. Synthetic peptides derived from the Listeria monocytogenes p60 protein as antigens for the generation of polyclonal antibodies specific for secreted cell-free L. monocytogenes p60 proteins
7. Structural and functional properties of the p60 proteins from different Listeria species
8. Transcription activation by catabolite activator protein (CAP)
9. Transcription activation at class II CAP-dependent promoters
10. Immunization and affinity purification of antibodies using resin-immobilized lysine-branched synthetic peptides
11. Differential interaction of the transcription factor PrfA and the PrfA-activating factor (Paf) of Listeria monocytogenes with target sequences
12. Internalin-mediated invasion of epithelial cells by Listeria monocytogenes is regulated by the bacterial growth state, temperature and the pleiotropic activator prfA
13. Sequence comparison of the chromosomal regions encompassing the internalin C genes (inlC) of Listeria monocytogenes and L. ivanovii
14. A new PrfA-regulated gene of Listeria monocytogenes encoding a small, secreted protein which belongs to the family of internalins
15. Listeria monocytogenes, a food-borne pathogen
16. Regulation of the prfA transcriptional activator of Listeria monocytogenes: Multiple promoter elements contribute to intracellular growth and cell-to-cell spread
17. Regulation of virulence genes in pathogenic Listeria
18. Listeria monocytogenes p60 supports host cell invasion by and in vivo survival of attenuated Salmonella typhimurium
19. Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral proteases
20. The gene coding for protein p60 of Listeria monocytogenes and its use as a specific probe for Listeria monocytogenes
21. Transcriptional regulation by cAMP and its receptor protein
22. Recombinant plasmids capable of replication in B. subtilis and E. coli
23. Virulence gene clusters and putative pathogenicity islands in Listeria
24. Molecular studies on the virulence of Listeria monocytogenes
25. Identification of an extracellular protein of Listeria monocytogenes possibly involved in intracellular uptake by mammalian cells
26. Hemolysin supports survival but not entry of the intracellular bacterium Listeria monocytogenes
27. Electroblotting of multiple gels: A simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose
28. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
29. The virulence regulator protein of Listeria ivanovii is highly homologous to PrfA from Listeria monocytogenes and both belong to the Crp-Fnr family of transcription regulators
30. The leucine zipper: A hypothetical structure common to a new class of DNA binding proteins
31. Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of Listeria monocytogenes
32. Expression of the Listeria monocytogenes EGD inlA and inlB genes, whose products mediate bacterial entry into tissue culture cell lines, by PrfA-dependent and -independent mechanisms
33. Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated
34. Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation
35. Characterization of a large motility gene cluster containing the cheR, motAB genes of Listeria monocytogenes and evidence that PrfA downregulates motility genes
36. Direct sequence identification and kinetic analysis of an MHC class I-restricted Listeria monocytogenes CTL epitope
37. Listeria monocytogenes-vaccine: Production and control
38. Evidence that PrfA, the pleiotropic activator of virulence genes in Listeria monocytogenes, can be present but inactive
39. Evidence for expressional crosstalk between the central virulence regulator PrfA and the stress response mediator ClpC in Listeria monocytogenes
40. A Gly145Ser substitution in the transcriptional activator PrfA causes constitutive overexpression of virulence factors in Listeria monocytogenes
41. Glucose-1-phosphate utilization by Listeria monocytogenes is PrfA dependent and coordinately expressed with virulence factors
42. Cell-surface location of Listeria-specific protein p60 - Detection of Listeria cells by indirect immunofluorescence
43. A single substitution in the putative helix-turn-helix motif of the pleiotropic activator PrfA attenuates Listeria monocytogenes virulence
44. Differential activation of virulence gene expression by PrfA, the Listeria monocytogenes virulence regulator
45. Surface-associated, PrfA-regulated proteins of Listeria monocytogenes synthesized under stress conditions
46. An assessment of prediction methods for locating continuous epitopes in proteins
47. Pathogenicity islands and virulence evolution in Listeria
48. Listeria pathogenesis and molecular virulence determinants
49. Functional similarities between the Listeria monocytogenes virulence regulator PrfA and cyclic AMP receptor protein: The PrfA* (Gly145Ser) mutation increases binding affinity for target DNA
50. In situ detection of a virulence factor mRNA and 16S rRNA in Listeria monocytogenes
51. The role of two surface exposed loops in transcription activation by the Escherichia coli CRP and FNR proteins
52. The iap gene of Listeria monocytogenes is essential for cell viability, and its gene product, p60, has bacteriolytic activity
53. Gene disruption by plasmid integration in Listeria monocytogenes: Insertional inactivation of the listeriolysin determinant lisA
54. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors