A single substitution in the putative helix-turn-helix motif of the pleiotropic activator PrfA attenuates Listeria monocytogenes virulence

Molecular Microbiology

Volumn 20, Issue 4, 1996, Pages 785-797

  Sheehan, Brian ^a,c   Klarsfeld, André ^a,d   Ebright, Richard ^b   Cossart, Pascale ^a  

^a INSTITUT PASTEUR   (France)

^b RUTGERS UNIVERSITY   (United States)

^c TRINITY COLLEGE DUBLIN   (Ireland)

^d CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

DNA BINDING PROTEIN;

ARTICLE; BACTERIAL VIRULENCE; CONTROLLED STUDY; DNA BINDING; GENE EXPRESSION REGULATION; LISTERIA MONOCYTOGENES; NONHUMAN; PRIORITY JOURNAL; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; CYCLIC AMP RECEPTOR PROTEIN; DNA; HELIX-TURN-HELIX MOTIFS; HEMOLYSIN FACTORS; KINETICS; LAC OPERON; LISTERIA MONOCYTOGENES; MICE; MOLECULAR SEQUENCE DATA; PEPTIDE TERMINATION FACTORS; SEQUENCE ALIGNMENT; STRUCTURE-ACTIVITY RELATIONSHIP; TRANS-ACTIVATORS;

EID: 0029978548     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2958.1996.tb02517.x     Document Type: Article

References (38)

1. Attey, A., Belyaeva, T., Savery, N., Hoggett, J., Fujita, N., Ishihama, A., and Busby, S. (1994) Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter. Nucl Acids Res 22: 4375-4380.
2. Cotter, P.P., and Miller, J.F. (1994) BvgAS-mediated signal transduction: analysis of phase-locked regulatory mutants of Bordetella bronchiseptica in a rabbit model. J Bacteriol 62: 3381-3390.
3. Dodd, I.B., and Egan, J.B. (1990) Improved detection of helix-turn-helix DNA-binding motifs in protein sequences. Nucl Acids Res 18: 5019-5026.
4. Dramsi, S., Dehoux, P., and Cossart, P. (1993a) Common features of Gram-positive bacterial proteins involved in cell recognition Mol Microbiol 9: 1119-1122.
5. Dramsi, S., Kocks, C., Forestier, C., and Cossart, P. (1993b) Internalin-mediated invasion of epithelial cells by Listeria monocytogenes is regulated by the bacterial growth state, temperature and the pleiotropic activator, prfA. Mol Microbiol 9: 931-941.
6. Dramsi, S., Biswas, I., Maguin, E., Braun, L., Mastroeni, P., and Cossart, P. (1995) Entry of Listeria monocytogenes into hepatocytes requires expression of InIB, a surface protein of the internalin multigene family. Mol Microbiol 16: 251-261.
7. Ebright, R.H. (1991) Identification of amino acid-base pair contacts by genetic methods. Meth Enzymol 208: 620-640.
8. Ebright, R.H., Cossart, P., Gicquel-Sanzey, B., and Beckwith, J. (1984a) Molecular basis of DNA sequence recognition by the catabolite gene activator protein: detailed inferences from three mutations that alter DNA sequence specificity. Proc Natl Acad Sci USA 81: 7274-7278.
9. Ebright, R.H., Cossart, P., Gicquel-Sanzey, B., and Beckwith, J. (1984b) Mutations that alter the DNA sequence specificity of the catabolite gene activator protein of E. coli. Nature 311: 232-235.
10. 181]CAP. Proc Natl Acad Sci USA 84: 6083-6087.
11. 1H NMR spectroscopy. EMBO J 13: 3936-3944.
12. Freitag, N.E., and Portnoy, D.A. (1994) Dual promoters of the Listeria monocytogenes prfA transcriptional activator appear essential in vitro but are redundant in vivo. Mol Microbiol 12: 845-853.
13. Freitag, N.E., Youngman, P., and Portnoy, D.A. (1992) Transcriptional activation of the Listeria monocytogenes hemolysin gene in Bacillus subtilis. J Bacteriol 174: 1293-1298.
14. Freitag, N.E., Rong, L., and Portnoy, D.A. (1993) Regulation of the prfA transcriptional activator of Listeria monocytogenes: multiple promoter elements contribute to intracellular growth and cell-to-cell spread. Infect Immun 61: 2537-2544.
15. Fried, M.G., and Crothers, D.M. (1981) Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucl Acids Res 9: 6505-6524.
16. Holm, L., Sander, C., Ruterjans, H., Schnarr, M., Fogh, R., Boelens, R., and Kaptein, R. (1994) LexA repressor and iron uptake regulator from Escherichia coli: new members of the CAP-like DNA binding domain superfamily. Prot Eng 7: 1449-1453.
17. Klarsfeld, A.D., Goossens, P.L., and Cossart, P. (1994) Five Listeria monocytogenes genes preferentially expressed in infected mammalian cells: plcA, purH, purD, pyrE and an arginine ABC transporter gene, arpJ. Mol Microbiol 13: 585-597.
18. Kunst, F., and Rapoport, G. (1995) Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis. J Bacteriol 177: 2403-2407.
19. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the heads of bacteriophage T4. Nature 227: 680-685.
20. Lampidis, R., Gross, R., Sokolovic, Z., Goebel, W., and Kreft, J. (1994) The virulence regulator protein of Listeria ivanovii is highly homologous to PrfA from Listeria monocytogenes and both belong to the Crp-Fnr family of transcription regulators. Mol Microbiol 13: 141-151.
21. Leimeister-Wächter, M., Haffner, C., Domann, E., Goebel, W., and Chakraborty, T. (1990) Identification of a gene that positively regulates expression of listeriolysin, the major virulence factor of Listeria monocytogenes. Proc Natl Acad Sci USA 87: 8336-8340.
22. Leimeister-Wächter, M., Domann, E., and Chakraborty, T. (1992) The expression of virulence genes in Listeria monocytogenes is thermoregulated. J Bacteriol 174: 947-952.
23. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1994) Interactions of wild-type and truncated LevR of Bacillus subtilis with the upstream activating region of the levanase operon. J Mol Biol 241: 178-192.
24. Mengaud, J., Vicente, M.F., and Cossart, P. (1989) Transcriptional mapping and nucleotide sequence of the Listeria monocytogenes hlyA region reveal structural features that may be involved in regulation. Infect Immun 57: 3695-3701.
25. Mengaud, J., Dramsi, S., Gouin, E., Vazquez-Boland, J.A., Milon, G., and Cossart, P. (1991a) Pleiotropic control of Listeria monocytogenes virulence factors by a gene which is autoregulated. Mol Microbiol 5: 2273-2283.
26. Mengaud, J., Geoffroy, C., and Cossart, P. (1991b) Identification of a novel operon involved in virulence of Listeria monocytogenes: its first gene encodes a protein homologous to bacterial metalloproteases. Infect Immun 59: 1043-1049.
27. Schultz, S., Shields, G., and Steitz, T. (1991) Crystal structure of a CAP-DNA complex: the DNA is bent by 90 . Science 253: 1001-1007.
28. Sheehan, B., Kocks, C., Dramsi, S., Gouin, E., Klarsfeld, A., Mengaud, J., and Cossart, P. (1994) Molecular and genetic determinants of the Listeria monocytogenes infectious process. Curr Top Microbiol Immun 192: 187-216.
29. Sheehan, B., Klarsfeld, A., Msadek, T., and Cossart, P. (1995) Differential activation of virulence gene expression by PrfA, the Listeria monocytogenes virulence regulator. J Bacteriol 177: 6469-6476.
30. Smith, K., and Youngman, P. (1992) Use of a new integrational vector to investigate compartment-specific expression of the Bacillus subtilis spollM gene. Biochimie 74: 705-711.
31. Spiro, S., and Guest, J.R. (1987) Activation of the lac operon of Escherichia coli by a mutant FNR protein. Mol Microbiol 1: 53-58.
32. Spiro, S., Gaston, K.L., Bell, A.I., Roberts, R.E., Busby, S.J., and Guest, J.R. (1990) Interconversion of the DNA-binding specificities of two related transcription regulators, CRP and FNR. Mol Microbiol 4: 1831-1838.
33. Vazquez-Boland, J.-A., Kocks, C., Dramsi, S., Ohayon, H., Geoffroy, C., Mengaud, J., and Cossart, P. (1992) Nucleotide sequence of the lecithinase operon of Listeria monocytogenes and possible role of lecithinase in cell-to-cell spread. Infect Immun 60: 219-230.
34. Vicente, M.F., Baquero, F., and Perez-Diaz, J.C. (1985) Cloning and expression of the Listeria monocytogenes haemolysin in E. coli. FEMS Microbiol Lett 30: 77-79.
35. West, D., Williams, R., Rhodius, V., Bell, A., Sharma, N., Zou, C., Fujita, N., Ishihama, A., and Busby, S. (1993) Interactions between the Escherichia coli cyclic AMP receptor protein and RNA polymerase at Class II promoters. Mol Microbiol 10: 789-797.
36. Zhang, X., and Ebright, R.H. (1990) Identification of a contact between arginine-180 of the catabolite gene activator protein (CAP) and base pair 5 of the DNA site in the CAP-DNA complex. Proc Natl Acad Sci USA 87: 4717-4721.
37. Zhou, Y., Merkel, T.J., and Ebright, R.H. (1994a) Characterization of the activating region of Escherichia coli catabolite gene activator protein (CAP) II. Role at Class I and Class II CAP-dependent promoters. J Mol Biol 243: 603-610.
38. Zhou, Y., Shannon Prendergast, P., Bell, A., Williams, R., Busby, S., and Ebright, R.H. (1994b) The functional subunit of a dimeric transcription activator protein depends on promoter architecture. EMBO J 13: 4549-4557.