The use of genetically engineered cells for assessing CYP2D6-related polymorphic effects

Toxicology in Vitro

Volumn 15, Issue 4-5, 2001, Pages 553-556

  Coecke, S ^a   Bogni, A ^b   Langezaal, I ^a   Worth, A ^a   Hartung, T ^b   Monshouwer, M ^c  

^a JOINT RESEARCH CENTRE   (Italy)

^b UNIVERSITY OF KONSTANZ   (Germany)

^c PFIZER INC   (United States)

Author keywords

Genetically engineered cell lines; Human CYP2D6 alleles; In vitro; Metabolism; Polymorphism

Indexed keywords

CYTOCHROME P450 2D6;

ANIMAL; ANIMAL CELL; BIOTECHNOLOGY; CELL STRAIN V 79; CHINESE HAMSTER; CONFERENCE PAPER; CONTROLLED STUDY; GENETIC ENGINEERING; GENETIC POLYMORPHISM; METABOLISM; NONHUMAN; PREDICTION; RISK ASSESSMENT;

EID: 0034826926     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(01)00061-3     Document Type: Conference Paper

References (11)

1. The integrated use of alternative methods in toxicological risk evaluation. ECVAM Integrated Testing Strategies Task Force Report 1
2. The use of long-term hepatocyte cultures for detecting induction of drug metabolising enzymes: The current status. ECVAM Hepatocytes and Metabolically Competent Systems Task Force Report 1
3. The integrated use of a genetically engineered cell line panel harbouring drug metabolising enzymes to detect metabolism-related problem compounds
4. C Novel approach to predicting P450-mediated drug metabolism: Development of a combined protein and pharmacophore model for CYP2D6
5. The decreased in vivo clearance of CYP2D6 substrates by CYP2D6*10 might be caused not only by the low-expression but also by low affinity of CYP2D6
6. High-performance liquid chromatographic assays for bufuralol 1′-hydroxylase, debrisoquine 4-hydroxylase, and dextromethorphan O-demethylase in microsomes and purified cytochrome P-450 isozymes of human liver
7. Development of a semi-automated in vitro assay to predict hepatic clearance of potential drug candidates using hepatocytes
8. Use of V79 cells with stably transfected cytochrome P450 cDNAs in studying the metabolism and effects of cytotoxic drugs
9. Preclinical drug metabolism in the age of high-throughput screening: An industrial perspective
10. Stable expression and coexpression of human cytochrome P450 oxidoreductase and cytochrome P450 1A2 in V79 Chinese hamster cells: Sensitivity to quinones and biotransformation of 7-alkoxyresorufins and triazines
11. Genetic polymorphisms of human N-acetyltransferase, cytochrome P450, glutathione-S-transferase, and epoxide hydrolase enzymes: Relevance to xenobiotic metabolism and toxicity