The polypeptide chain release factor eRF1 specifically contacts the s4UGA stop codon located in the A site of eukaryotic ribosomes

European Journal of Biochemistry

Volumn 268, Issue 10, 2001, Pages 2896-2904

  Chavatte, Laurent ^a   Frolova, Ludmila ^b,c   Kisselev, Lev ^b,c   Favre, Alain ^a  

^a INSTITUT JACQUES MONOD   (France)

^b ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

^c INSTITUT CURIE   (France)

Author keywords

ERF1; ERF3; Eukaryotes; s4U photocrosslinking; Translation termination

Indexed keywords

BIOLOGICAL FACTOR; MESSENGER RNA; POLYPEPTIDE CHAIN RELEASE FACTOR ERF1; RIBOSOME RNA; TRANSFER RNA; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CODON; CONTROLLED STUDY; EUKARYOTE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RIBOSOME;

ANIMALS; CODON, TERMINATOR; CROSS-LINKING REAGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; MAGNESIUM; PEPTIDE TERMINATION FACTORS; PLASMIDS; PROTEIN BINDING; PROTEIN BIOSYNTHESIS; RABBITS; RIBOSOMES; RNA, MESSENGER; RNA, TRANSFER, ASP; RNA, TRANSFER, MET; SALTS;

EUKARYOTA; PROKARYOTA;

EID: 0034826629     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02177.x     Document Type: Article

References (41)

1. Hidden infidelities of the translational stop signal
2. Polypeptide chain release factors
3. How protein reads the stop codon and terminates translation
4. Translational termination comes of age
5. Genetic screen for cloned release factor genes
6. Eukaryotic release factor 1 (eRF1) abolishes readthrough and competes with suppressor tRNAs at all three termination codons in messenger RNA
7. Overexpression of human release factor 1 alone has an antisuppressor effect in human cells
8. A single proteolytic cleavage in release factor 2 stabilizes ribosome binding and abolishes peptidyl-tRNA hydrolysis activity
9. 4-Thiouridine as an intrinsic photoaffinity probe of nucleic acid structure and interactions
10. Thionucleobases as intrinsic photoaffinity probes of nucleic acid structure and nucleic acid-protein interactions
11. Codon recognition in polypeptide chain termination: Site directed crosslinking of termination codon to Escherichia coli release factor 2
12. Direct recognition of mRNA stop signals by Escherichia coli polypeptide chain release factor two
13. Decoding the translational termination signal: The polypeptide chain release factor in Escherichia coli crosslinks to the base following the stop codon
14. Peptide chain termination with mammalian release factor
15. Characterization of reticulocyte release factor
16. A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor
17. Class-1 polypeptide chain release factors are structurally and functionally similar to suppressor tRNAs and comprise different structural-functional families of prokaryotic/mitochondrial and eukaryotic/archaebacterial factors
18. Mutations in the highly conserved GGQ motif of class 1 polypeptide release factors abolish ability of human eRF1 to trigger peptidyl-tRNA hydrolysis
19. Eukaryotic polypeptide release factor eRF1 is composed of two functionally and structurally distinct domains
20. The crystal structure of human eukaryotic release factor eRF1-mechanism of stop codon recognition and peptidyl-tRNA hydrolysis
21. C-terminal domains of human translation termination factors eRF1 and eRF3 mediate their in vivo interaction
22. Terminating eukaryote translation: Domain 1 of release factor eRF1 functions in stop codon recognition
23. Arrangement of mRNA at the decoding site of human ribosomes. 18S rRNA nucleotides and ribosomal proteins cross-linked to oligouridylate derivatives with alkylating groups at either the 3′ or the 5″ termini
24. Protein environment at the decoding site of 80S ribosomes from human placenta as revealed from affinity labeling with mRNA analogs derivatives of oligoribonucleotides
25. Nucleotide G-1207 of 18S rRNA is an essential component of the human 80S ribosomal decoding center
26. Cross-linking of mRNA analogues containing 4-thiouridine residues on the 3′- or 5′-side of the coding triplet to the mRNA binding center of the human ribosome
27. mRNA binding track in the human 80S ribosome for mRNA analogues randomly substituted with 4-thiouridine residues
28. Interaction of tRNAs and phosphorothioate-substituted nucleic acids with a organomercurial. Probing the chemical environment of thiolated residues by affinity electrophoresis
29. Triple aminoacylation specificity of a chimerized transfer RNA
30. Synthesis of small RNAs using T7 RNA polymerase
31. Conformation and structural fluctuations of a 218 nucleotides long rRNA fragment: 4-Thiouridine as an intrinsic photolabelling probe
32. Functional expression of eukaryotic polypeptide chain release factors 1 and 3 by means of baculovirus/insect cells and complex formation between the factors
33. Termination of protein synthesis
34. Ribosomal binding site of release factors RF1 and RF2. A new translational termination assay in vitro
35. Interaction of human and Escherichia coli tRNA (Phe) with human 80S ribosomes in the presence of oligo-and polyuridylate templates
36. Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3
37. Eukaryotic polypeptide chain release factor eRF3 is an eRF1- and ribosome-dependent guanosine triphosphatase
38. A tripeptide anticodon deciphers stop codons in messenger RNA
39. Mimicry grasps reality in translation termination
40. Efficient in vitro translational termination in Escherichia coli is constrained by the orientations of the release factor, stop signal and peptidyl-tRNA within the termination complex
41. Nucleotides of 18S rRNA surrounding mRNA codons at the human ribosomal A, P and E sites: A crosslinking study with mRNA analogs carrying an aryl azide group at either the uracil or the guanine residue