Functional expression of eukaryotic polypeptide chain release factors 1 and 3 by means of baculovirus/insect cells and complex formation between the factors

European Journal of Biochemistry

Volumn 256, Issue 1, 1998, Pages 36-44

  Frolova, Lyudmila Y ^a,b,c   Simonsen, Janne L ^a   Merkulova, Tatyana I ^b   Litvinov, Dmitry Y ^c   Martensen, Pia M ^a   Rechinsky, Vladimir O ^c   Camonis, Jacques H ^b   Kisselev, Lev L ^b,c   Justesen, Just ^a  

^a AARHUS UNIVERSITY   (Denmark)

^b INSTITUT CURIE   (France)

^c ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

Author keywords

Baculovirus; Complex formation; Human; Limited proteolysis; Translation termination

Indexed keywords

MESSENGER RNA; POLYPEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; BACULOVIRUS; COMPLEX FORMATION; EUKARYOTE; GENE EXPRESSION SYSTEM; HUMAN; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; GENE EXPRESSION; HUMANS; MOLECULAR SEQUENCE DATA; MOTHS; NUCLEOPOLYHEDROVIRUS; PEPTIDE TERMINATION FACTORS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SERINE ENDOPEPTIDASES;

EUKARYOTA; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0032529092     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2560036.x     Document Type: Article

References (43)

1. Kisselev, L. & Frolova, L. (1995) Termination of translation in eukaryotes, Biochem. Cell Biol. 73, 1079-1086.
2. Buckingham, R. H., Grentzmann, G. & Kisselev, L. (1997) Polypeptide chain release factors, Mol. Microbiol. 24, 449-456.
3. Drugeon, G., Jean-Jean, O., Le Goff, X., Frolova, L., Philippe, M., Kisselev, L. & Haenni, A.-L. (1997) Eukaryotic release factor 1 (eRF1) abolishes read-through and competes with suppressor tRNAs at all three termination codons in messenger RNA, Nucleic Acids Res. 25, 2254-2258.
4. Caskey, C. T. (1980) Peptide chain termination, Trends Biochem. Sci. 5, 234-237.
5. Frolova, L., Le Goff, X., Rasmussen, H. H., Cheperegin, S., Drugeon, G., Kress, M., Arman, I., Haenni, A.-L., Celis, J. E., Philippe, M., Justesen, J. & Kisselev, L. (1994) A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor, Nature 372, 701-703.
6. Tate, W. P. & Caskey, C. T. (1974) Polypeptide chain termination, in The enzymes (Boyer, P. D., ed.) vol. 10, pp. 87-118, Academic Press, New York.
7. Zhouravleva, G., Frolova, L., Le Goff, X., Le Guellec, R., Inge-Vechtomov, S., Kisselev, L. & Philippe, M. (1995) Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3, EMBO J. 14, 4065-4072.
8. Frolova, L., Le Goff, X., Zhouravleva, G., Davydova, E., Philippe, M. & Kisselev, L. (1996) Eukaryotic polypeptide chain release factor eRF3 is an eRF1- and ribosome-dependent guanosine triphosphatase, RNA 2, 334-341.
9. Breining, P. & Piepersberg, W. (1986) Yeast omnipotent suppressor SUP1 (SUP45): nucleotide sequence of the wild type and mutant gene, Nucleic Acids Res. 14, 5187-5197.
10. Kushnirov, V. V., Ter-Avanesyan, M. D., Telkov, M. V., Surguchov, A. P., Smirnov, V. N. & Inge-Vechtomov, S. G. (1988) Nucleotide sequence of the sup2 (sup35) gene of Saccharomyces cerevisiae, Gene (Amst.) 66, 45-54.
11. Hoshino, S.-I., Miyazawa, H., Enomoto, T., Hanaoka, F., Kikuchi, Y., Kikuchi, A. & Ui, M. (1989) A human homologue of the yeast GST1 gene codes for a GTP-binding protein and is expressed in a proliferation-dependent manner in mammalian cells, EMBO J. 8, 3807-3814.
12. Grenett, H. E., Bounelis, P. & Fuller, G. M. (1992) Identification of a human cDNA with high homology to yeast omnipotent suppressor 45, Gene (Amst.) 110, 239-243.
13. Tassan, J. P., Le Guellec, K., Kress, M., Faure M., Camonis, J., Jacquet, M. & Philippe, M. (1993) In Xenopus laevis, the product of a developmentally regulated mRNA is structurally and functionally homologous to a Saccharomyces cerevisiae protein involved in translation fidelity, Mol. Cell. Biol. 13, 2815-2821.
14. Jean-Jean, O., Le Goff, X. & Philippe, M. (1996) Is there a human [psi]? CR. Acad. Sci. Paris, Science de la vie/Life Sciences 319, 487-492.
15. Ito, K., Ebihara, K., Uno, M. & Nakamura, Y. (1996) Conserved motifs of prokaryotic and eukaryotic polypeptide chain release factors: tRNA-protein mimicry hypothesis, Proc. Natl Acad. Sci. USA 93, 5443-5448.
16. Lee, C. C., Craigen, W. J., Muzny, D. M., Harlow, E. & Caskey, T. C. (1990) Cloning and expression of a mammalian peptide chain release factor with sequence similarity to tryptophanyl-tRNA synthetase, Proc. Natl Acad. Sci. USA 87, 3508-3512.
17. Frolova, L. Y., Dalphin, M. E., Justesen, J., Powell, R. J., Drugeon, G., McCaughan, K. K., Kisselev, L. L., Tate, W. P. & Haenni, A.-L. (1993) Mammalian polypeptide chain release factor and tryptophanyl-tRNA synthetase are distinct proteins, EMBO J. 12, 4013-4019.
18. Nakamura, Y, Ito, K. & Isaksson, L. A. (1996) Emerging understanding of translation termination, Cell 87, 147-150.
19. Freistroffer, D. V., Pavlov, M. Y. MacDougall, J., Buckingham, R. H. & Ehrenberg, M. (1997) Release factor RF3 in E. coli accelerates the dissociation of release factors RF1 and RF2 from the ribosome in a GTP-dependent manner, EMBO J. 13, 4126-4133.
20. Pavlov, M. Y., Freistroffer, D. V., MacDougall, J., Buckingham, R. H. & Ehrenberg, M. (1997) Fast recycling of Escherichia coli ribosomes requires both ribosome recycling factor (RRF) and release factor RF3, EMBO J. 13, 4134-4141.
21. Pavlov, M. Y., Freistroffer, D. V., Heurgué-Hamard, V., Buckingham, R. H. & Ehrenberg, M. (1997) Release factor RF3 abolishes competition between release factor RF1 and ribosome recycling factor (RRF) for a ribosome binding site, J. Mol. Biol. 273, 389-401.
22. Stansfield, I., Jones, K. M., Kushnirov, V. V., Dagkesamanskaya, A. R., Poznyakovski, A. I., Paushkin, S. V., Nierras, C. R., Cox, B. S., Ter-Avanesyan, M. D. & Tuite, M. F. (1995) The product of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae, EMBO J. 14, 4365-4373.
23. Pel, H. J., Moffat, J. G., Ito, K., Nakamura, Y. & Tate, W. P. (1998) Escherichia coli release factor 3: resolving the paradox of a typical G protein structure and atypical function with guanine nucleotides, RNA 4, 47-54.
24. Andjelkovic, N., Zolnierowicz, S., Van Hoof, C., Goris, J. & Hemmings, B. A. (1996) The catalytic subunit of the protein phosphatase 2A associates with the translation termination factor eRF1, EMBO J. 15, 7156-7167.
25. Paushkin, S. V., Kushnirov, V. V., Smirnov, V. N. & Ter-Avanesyan, M. D. (1997) Interaction between yeast Sup45p (eRF1) and Sup35p (eRF3) polypeptide chain release factors: implications for prion-dependent regulation, Mol. Cell. Biol. 17, 2798-2805.
26. Stansfield, I., Kushnirov, V. V., Jones, K. M. & Tuite, M. F. (1997) A conditional-lethal translation termination defect in a sup45 mutant of the yeast Saccharomyces cerevisiae, Eur. J. Biochem. 245, 557-563.
27. Eldridge, M. G. & Dailey, H. A. (1992) Yeast ferrochelatase: expression in baculovirus system and purification of the expression protein. Protein Sci. 1, 271-277.
28. O'Reilly, D. R., Miller, L. K. & Luckow, V. A. (1992) Baculovirus expression vectors, a laboratory manual, W. H. Freeman and Company, New York.
29. Yet, S. F., Moon, Y. K. & Sul, H. S. (1995) Purification and reconstitution of murine mitochondrial glycerol-3-phosphate acyltransferase. Functional expression in baculovirus-infected insect cells, Biochemistry 34, 7303-7310.
30. Knecht, W., Bergjohann, U., Gonski, S., Kirschbaum, B. & Loffler, M. (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme, Eur. J. Biochem. 240, 292-301.
31. Gruenwald, S. & Heitz, J. (1993) Baculovirus expression vector system: procedures and methods manual 2nd edn. PharMingen, San Diego.
32. King, L. A. & Possee, R. D. (1992). The baculovirus expression system: a laboratory guide, Chapman & Hall, London.
33. Le Goff, X., Philippe, M. & Jean-Jean, O. (1997) Overexpression of human release factor 1 alone has a antisuppressor effect in human cells, Mol. Cell. Biol. 17, 3164-3172.
34. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
35. Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl Acad. Sci. USA 76, 4330-4354.
36. Harlow, E. & Lane, D. (1988) Antibodies: a laboratory manual, Cold Spring Harbour Laboratory Press, Cold Spring Harbour, NY.
37. Frolova, L. Y., Fleckner, J., Justesen, J., Timms, K. M., Tate, W. P., Kisselev, L. L. & Haenni, A.-L. (1993) Are the tryptophanyl-tRNA synthetase and the peptide chain release factor from higher eukaryotes one and the same protein? Eur. J. Biochem. 212, 457-466.
38. Cox, R. A. & Hirst, W. (1976). A study of the influence of magnesium ions on the conformation of ribosomal ribonucleic acid and on the stability of the larger subribosomal particle of rabbit reticulocytes, Biochem. J. 160, 505-519.
39. Caskey, C. T., Beaudet, A. L. & Tate, W. P. (1974) Mammalian release factor: in vitro assay and purification, Methods Enzymol. 30, 293-303.
40. Zalcman, G., Closson, V., Camonis, J., Honore, N., Rousseau-Merck, M. F., Tavilian, A. & Olofsson, B. (1996) RhoGDl-3 is a new GDP dissociation inhibitor (GDI). Identification of a non-cytosolic GDI protein interacting with the small GTP-binding proteins RhoB and RhoG, J. Biol. Chem. 271, 30366-30374.
41. Vojtek, A. B., Hollenberg, S. M. & Cooper, J. A. (1993) Mammalian Ras interacts directly with serine/threonine kinase Raf, Cell 74, 205-214.
42. Hannon, G. J., Demetrick, D. & Beach, D. (1993) Isolation of the Rb-related p130 through its interaction with CDK2 and cyclins, Genes and Dev. 7, 2378-2391.
43. Jullien-Flores, V., Dorseuil, O., Romero, F., Letourneur, F., Saragosti, S., Berger, R., Tavitian, A., Gacon, G. & Camonis, J. H. (1995) Bridging Ral GTPase to Rho pathways. RLIP76, a Ral effector with CDC42/Rac GTPase-activating protein activity, J. Biol. Chem. 270, 22473-22477.