Solution structure of cytochrome b5 mutant (E44/48/56A/D60A) and its interaction with cytochrome c

European Journal of Biochemistry

Volumn 268, Issue 6, 2001, Pages 1620-1630

  Wu, Yibing ^a,c   Wang, Yunhua ^b   Qian, Chengmin ^a   Lu, Jun ^c   Li, Ercheng ^d   Wang, Wenhu ^b   Lu, Junxia ^b   Xie, Yi ^b   Wang, Jinfeng ^d   Zhu, Dexi ^c   Huang, Zhongxian ^b   Tang, Wenxia ^a  

^a NANJING UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

^c NANJING UNIVERSITY   (China)

^d INSTITUTE OF BIOPHYSICS   (China)

Author keywords

Cytochrome b5; Cytochrome c; Electrostatic interaction; Mutagenesis; Solution structure

Indexed keywords

CYTOCHROME B5; CYTOCHROME C; MUTANT PROTEIN;

ARTICLE; BINDING SITE; COMPARATIVE STUDY; COMPLEX FORMATION; ELECTRICITY; ENZYME SPECIFICITY; ENZYME STABILITY; GEOMETRY; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

AMINO ACID SEQUENCE; CYTOCHROME C GROUP; CYTOCHROMES B5; MAGNETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION;

EID: 0034825436     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02033.x     Document Type: Article

References (54)

1. Electron transfer in biological system: An overview
2. Electron transfers in chemistry and biology
3. Cytochrome c as an electron shuttle between the outer an inner mitochondrial membranes
4. 5 to cytochrome
5. 5
6. 5-cytochrome c complex
7. 5-cytochrome c complex
8. 5 through mutagenesis and molecular modelling
9. 5
10. 5
11. Mapping electrostatic interactions in macromolecular associations
12. 5
13. 5
14. Mitochondrial cytochrome c: Preparation and activity of native and chemically modified cytochromes c
15. The relevance of cross-peaks in two-dimensional NOE experiments of macromoleculars
16. Application of phase sensitive correlated spectroscopy (COSY) for measurements of proton-proton spin-spin coupling constants in proteins
17. MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy
18. 1H NMR spectra of proteins via double quantum filtering
19. Rapid-pulsing artifacts in double-quantum-filtered cosy
20. Efficient detection of paramagnetically shifted NMR resonances by optimizing the WEFT pulse sequence
21. 1H NOE studies on dicopper (II) dicobalt (II) superoxide dismutase
22. Efficient analysis of protein 2D NMR spectra using the software package EASY
23. Efficient computation of three-dimensional protein structures in solution from nuclear magnetic resonance data using the program DIANA and the supporting programs CALIBA, HABAS and GLOMSA
24. Isotropic NMR shifts in transition metal complexes: Calculation of the Fermi contact and pseudocontact terms
25. 5
26. 5 obtained from pig and calf
27. PSEUDODYANA for NMR structure calculation of paramagnetic metalloproteins using torsion angle molecular dynamics
28. Torsion angle dynamics for NMR structure calculation with the new program DYANA
29. 5
30. The use of pseudocontact shifts to refine solution structures of paramagnetic metalloproteins: Met80Ala cyano-cytochrome c as an example
31. The pseudocontact shifts as constriants for energy minimization and molecular dynamic calculations on solution structures of paramagnetic metalloproteins
32. AQUA and PROCHECK-NMR: Programs for checking the quality of protein structures solved by NMR
33. PROCHECK: A program to check the stereochemical quality of protein structures
34. 5, its E44A and/or E56A mutants and cytochrome
35. 5 by 2D NMR spectroscopy
36. 5 obtained from pig and calf
37. 5 based on individual assignments of the NMR lines of the heme group and selected amino acid residues
38. 5: Assignment of heme propionate resonance on the basic of nuclear oberhauser effect measurements and the nature of interprotein contacts with parter redox proteins
39. 5 in solution
40. 5 on terms of angular position of the heme: A sensitive probe for peripheral heme protein interactions
41. 5 at 2.8 Å resolution
42. 5 determined using hetero-nuclear NMR methods
43. 5
44. 5 at 1.5 Å resolutoin
45. 5
46. Determination of haem electronic structure in His-Met cytochromes c by 13C NMR: The effect of the axial ligands
47. Co- and counter-rotation of magnetic axes and axial ligands in low-spin ferriheme systems
48. 5
49. 5 heme propinates in the electrostatic binding to cytochrome c
50. 5 and cytochrome c
51. 5 reductase
52. MOLMOL: A program for display and analysis of macromolecular structure