Improving the solubility of the catalytic domain of human β-1,4-galactosyltransferase 1 through rationally designed amino-acid replacements

European Journal of Biochemistry

Volumn 268, Issue 15, 2001, Pages 4352-4358

  Malissard, Martine ^a   Berger, Eric G ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

1,4 galactosyltransferase; Heterologous expression; Protein solubility; Site directed mutagenesis

Indexed keywords

AMINO ACID; BETA 1,4 GALACTOSYLTRANSFERASE 1; GALACTOSE; GALACTOSYLTRANSFERASE; ISOPROTEIN; MUTANT PROTEIN; N ACETYLGLUCOSAMINE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE;

AMINO ACID SUBSTITUTION; ARTICLE; BREAST MILK; CATALYSIS; CHROMATOGRAPHY; CLONING; CONTROLLED STUDY; DEGLYCOSYLATION; ENZYME ACTIVE SITE; ENZYME ASSAY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION; HUMAN; IMMUNOBLOTTING; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS; SOLUBILITY; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; CATALYTIC DOMAIN; CATTLE; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GALACTOSYLTRANSFERASES; GLYCOSYLATION; HUMANS; KINETICS; MICE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; PROTEIN ISOFORMS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID; SOLUBILITY;

EID: 0034819505     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02357.x     Document Type: Article

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