Structure determination of biological macromolecules in solution using nuclear magnetic resonance spectroscopy

BioTechniques

Volumn 29, Issue 6, 2000, Pages 1278-1294

  Wider, G ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CONFORMATION; CRYSTAL STRUCTURE; MACROMOLECULE; MOLECULAR DYNAMICS; MOLECULAR SIZE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REVIEW; X RAY DIFFRACTION;

EID: 0034535525     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00296ra01     Document Type: Review

References (61)

1. Two-dimensional spectroscopy. Application to nuclear magnetic resonance
2. Methodological advances in protein NMR
3. The protein data bank: A computer-based archival file for macromolecular structures
4. Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopy
5. Distance geometry and related methods for protein structure determination from NMR data
6. 15N 'random coil' chemical shifts
7. Computational challenges for macromolecular structure determination by X-ray and solution NMR spectroscopy
8. Structural proteomics of an archaeon
9. Determining the structures of large proteins and protein complexes by NMR
10. 3(h)J(NC) scalar couplings
11. New approaches to structure determination by NMR spectroscopy
12. Insights into protein folding from NMR
13. Characterization of the backbone dynamics of folded and denatured states of an SH3 domain
14. The use of H-2, C-13, N-15 multidimensional NMR to study the structure and dynamics of proteins
15. Solution structure of the 40,000 M phosphoryl transfer complex between the N-terminal domain of enzyme I and HPr
16. Improved 3D triple-resonance NMR techniques applied to a 31 kDa protein
17. 2O in protein NMR. Application to sensitivity enhancement and NOE measurements
18. Structure calculation of biological macromolecules from NMR data
19. Efficient computation of three-dimensional protein structures in solution from nuclear magnetic resonance data using the program DIANA and the supporting programs CALIBA, HABAS and GLOMSA
20. Torsion angle dynamics for NMR structure calculation with the new program DYANA
21. Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions
22. An evaluation of computational strategies for use in the determination of protein structure from distance constraints obtained by nuclear magnetic resonance
23. 3 domain of the human p56 Lck tyrosine kinase
24. Investigation of exchange processes by two-dimensional NMR spectroscopy
25. Protein dynamics from NMR
26. Solution NMR spectroscopy beyond 25 kDa
27. A 2D NOE experiment for the elucidation of complete proton-proton cross-relaxation networks in biological macromolecules
28. Statistical basis for the use of (13)Cα chemical shifts in protein structure determination
29. Global folds of proteins With low densities of NOEs using residual dipolar couplings: Application to the 370-residue maltodextrin-binding protein
30. Expressed protein ligation: A general method for protein engineering
31. Determination of three-dimensional structures of proteins from interproton distance data by hybrid distance geometry-dynamical simulated annealing calculations
32. Improved segmental isotope labeling of proteins and application to a larger protein
33. Probing molecular motion by NMR
34. 2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution
35. 13C-labeled proteins
36. The 3D NOESY-[(1)H,(15)N, 1H]- ZQ-TROSY NMR experiment with diagonal peak suppression
37. New techniques in structural NMR anisotropic interactions
38. TROSY in triple-resonance experiments: New perspectives for sequential NMR assignment of large proteins
39. NMR Assignment and secondary structure determination of an octameric 110 kDa protein using TROSY in triple resonance experiments
40. TROSY-type triple-resonance experiments for sequential assignments of large proteins
41. Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients
42. Relaxation processes in a system of two spins
43. 13C nuclear-magnetic-resonance chemical-shifts
44. Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium
45. Technical aspects of NMR spectroscopy with biological macromolecules and studies of hydration in solution
46. NMR spectroscopy of large molecules and multimolecular assemblies in solution
47. 2O solution
48. 1H NMR of proteins. Experimental Procedures
49. 13C chemical-shift data
50. Biological macromolecules: Structure determination in solution
51. NMR - An alternative to x-ray cristallography for protein and nucleic acid structure determination
52. The second decade - Into the third millennium
53. NMR studies of the hydration of biological macromolecules
54. Chemical ligation of folded recombinant proteins: Segmental isotopic labeling of domains for NMR studies
55. 2H-labeled proteins: Application to a 37kDa Trp repressor-DNA complex
56. Segmental isotope labeling for protein NMR using peptide splicing
57. Extending the size limit of protein nuclear magnetic resonance