Overexpression, refolding, and purification of the histidine-tagged outer membrane efflux protein OprM of Pseudomonas aeruginosa

Protein Expression and Purification

Volumn 23, Issue 1, 2001, Pages 121-127

  Charbonnier, Franck ^a   Köhler, Thilo ^b   Pechère, Jean Claude ^b   Ducruix, Arnaud ^a  

^a CNRS   (France)

^b UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; GENE OVEREXPRESSION; GENE TAGGING; GUANIDINE HYDROCHLORIDE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMUNOBLOTTING; N OCTYLPOLYOXYETHYLENE; N OCTYLTETRAOXYETHYLENE; OUTER MEMBRANE PROTEIN; POLYHISTIDINE; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA; RENATURATION; SIGNAL PEPTIDE;

EID: 0034816414     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1473     Document Type: Article

References (39)

1. Antibiotic efflux, a mechanism of multiple resistance in Pseudomonas aeruginosa
2. Multidrug efflux pumps of Gram-negative bacteria
3. emr, an Escherichia coli locus for multidrug resistance
4. Resistance of Neisseiria gonorrheae to Antimicrobial hydrophobic agents is modulated by the mtrCDE efflux system
5. Evidence for an efflux pump in multidrug-resistant Campylobacter jejuni
6. Multiple antibiotic resistance in Stenotrophomonas maltophilia: Involvement of a multidrug efflux system
7. Multidrug efflux in intrinsic resistance to trimethoprim and sulfamethazole in Pseudomonas aeruginosa
8. Overexpression of the mexC-mexD-oprJ efflux operon in nfxBtype multidrug-resistant strains of Pseudomonas aeruginosa
9. Characterization of MexEMexF-OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosa
10. Involvement of an active efflux system in the natural resistance of Pseudomonas aeruginosa to aminoglycosides
11. Multiple antibiotic resistance in Pseudomonas aeruginosa: Evidence for involvement of an efflux operon
12. Role of MexA-MexB-OprM in antibiotic efflux in Pseudomonas aeruginosa
13. OprK and OprM define two genetically distinct multidrug efflux systems in Pseudomonas aeruginosa
14. Carbapenem activities against Pseudomonas aeruginosa: Respective contributions of OprD and efflux systems
15. New norfloxacin resistance gene in Pseudomonas aeruginosa PAO
16. Influence of the MexAB-OprM multidrug efflux system on quorum sensing in Pseudomonas aeruginosa
17. Localization of the outer membrane subunit OprM of resistance-nodulation-cell-division-family multicomponent efflux pump in Pseudomonas aeruginosa
18. Insertion mutagenesis and membrane topology model of the Pseudomonas aeruginosa outer membrane protein OprM
19. Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: Regulation by iron and possible involvement in the secretion of the siderophore pyoverdine
20. Machine learning approaches for the prediction of signal peptides and other protein sorting signals
21. A rapid and sensitive method for the quantitation of microgram quantities of proteins using the principle of protein-dye binding
22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
23. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation
24. Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI
25. Structure of the outer membrane protein A transmembrane domain
26. Expression of porin from Rhodopseudomonas blastica in Escherichia coli inclusion bodies and folding into exact native structure
27. Cyanate formation in solutions with urea
28. Effects on heating in dodecylsulfate solution on the conformation and electrophoretic mobility of isolated outer membrane proteins from Escherichia coli K12
29. Crystal structures explain functional properties of two E. coli porins
30. Porin isolated from the cell envelope of Rhodopseudomonas capsulata
31. Alignment and structure prediction of divergent protein families: Periplasmic and outer membrane proteins of bacterial efflux pumps
32. β-barrel proteins from bacterial outer membranes: Structure, function and refolding
33. Membrane proteins
34. The three dimensional structure of porin from Rhodobacter capsulatus at 3 Å resolution
35. The crystal structure of outer membrane phospholipase, an integral membrane enzym
36. Siderophore-mediated iron transport: Crystal structure of FhuA with bound lipopolysaccharide
37. Crystal structure of the outer membrane active transporter FepA from Escherichia coli
38. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export