Redistribution of metabolic fluxes in Escherichia coli with fermentative lactate dehydrogenase overexpression and deletion

Metabolic Engineering

Volumn 1, Issue 2, 1999, Pages 141-152

  Yang, Yea Tyng ^a   San, Ka Yiu ^a   Bennett, George N ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

Intracellular pyruvate; Lactate dehydrogenase; Metabolic control coefficients; Metabolic engineering; Metabolic flux analysis

Indexed keywords

ESCHERICHIA COLI;

ACYLTRANSFERASE; FORMATE C ACETYLTRANSFERASE; FORMATE C-ACETYLTRANSFERASE; LACTATE DEHYDROGENASE; RECOMBINANT PROTEIN;

ANAEROBIC GROWTH; ARTICLE; CHEMICAL MODEL; CITRIC ACID CYCLE; ESCHERICHIA COLI; FERMENTATION; GENE DELETION; GENETIC ENGINEERING; GENETICS; GROWTH, DEVELOPMENT AND AGING; KINETICS; METABOLISM; MUTAGENESIS;

ACETYLTRANSFERASES; ANAEROBIOSIS; ESCHERICHIA COLI; FERMENTATION; GENE DELETION; GENETIC ENGINEERING; KINETICS; L-LACTATE DEHYDROGENASE; MODELS, CHEMICAL; MUTAGENESIS; RECOMBINANT PROTEINS; SUBSTRATE CYCLING;

EID: 0033107572     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.1998.0111     Document Type: Article

References (37)

1. Aristidou, A. A., San, K.-Y., and Bennett, G. N. (1994). Modification of central metabolic pathway in Escherichia coli to reduce acetate accumulation by heterologous expression of the Bacillus subtilis acetolactate synthase gene, Biotechnol. Bioeng. 44, 944-951.
2. Aristidou, A. A. (1994). "Application of Metabolic and Biochemical Engineering Techniques for the Enhancement of Recombinant Protein Production in Escherichia coli," Ph.D. thesis, Rice University, Houston, TX.
3. Bauer, K., Bassat, A. B., Dawson, M., De La Puente, V. T., and Neway, J. O. (1990). Improved expression of human interleukin-2 in high-cell-density fermentor cultures of Escherichia coli K-12 by a phosphotransacetylase mutant, Appl. Environ. Microbiol. 56, 1296-1302.
4. Bock, A., and Sawers, G. (1996). Fermentation. In "Escherichia coli and Salmonella: Cellular and Molecular Biology" (F. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasnik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger, Eds.), pp. 262-282, American Society for Microbiology, Washington, DC.
5. Bunch, P. K., Mat-Jan, F., Lee, N., and Clark, D. P. (1997). The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli, Microbiology 143, 187-195.
6. Crabtree, H. G. (1929). Observations on the carbohydrate metabolism of tumors, Biochem. J. 23, 536-545.
7. Chang, A. C. Y., and Cohen, S. N. (1978). Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the p15A cryptic miniplasmid, J. Bacteriol. 134, 1141-1156.
8. Chou, C.-H., Bennett, G. N., and San, K.-Y. (1994). Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in Escherichia coli dense cultures, Biotechnol. Bioeng. 44, 952.
9. Diaz-Ricci, J. C., Regan, L., and Bailey, J. E. (1991). Effect of alteration of the acetic acid synthesis pathway on the fermentation pattern of Escherichia coli, Biotechnol. Bioeng. 38, 1318-1324.
10. Doelle, H. W., Ewings, K. N., and Hollywood, N. W. (1981). Regulation of glucose metabolism in bacterial systems, Adv. Biochem. Eng./ Biotechnol. 23, 1-36.
11. Garvie, E. I. (1980). Bacterial lactate dehydrogenates, Microbiol. Rev. 44, 106-139.
12. Goldbeter, A., and Koshland, D. (1982). Sensitivity amplification in biochemical systems, Q. Rev. Biophys. 15, 555-591.
13. Grosz, R., and Stephanopoulos, G. (1990). Physiological, biochemical, and mathematical studies of micro-aerobic contiuous ethanol fermentation by Saccharomyces cervisiae. II. Intracellular metabolite and enzyme assays at steady state chemostat cultures, Biotechnol. Bioeng. 36, 1020-1029.
14. Iuchi, S. (1993). Phosphorylation/dephosphorylation of the receiver module at the conserved aspartate residue controls transphosphorylation activity of histidine kinase in sensor protein ArcB of Escherichia coli, J. Biol. Chem. 263, 23972-23980.
15. Iuchi, S., and Lin, E. C. C. (1992). Mutational analysis of signal transduction by ArcB: A membrane sensor protein for anaerobic expression of operons involve in the central aerobic pathways in Escherichia coli, J. Bacteriol. 174, 3972-3980.
16. Kaiser, M., and Sawers, G. (1997). Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli, Microbiology 143, 775-783.
17. Kessler, D., Herth, W., and Knappe, J. (1992). Ultra structure and pyruvate formate-lyase radical quenching property of the multienzymic adhE protein of Escherichia coli, J. Biol. Chem. 267, 18073-18079.
18. Knappe, J., Schacht, J., Mochel, W., Hopner, T., Vetter, J., and Edenharder, R. (1969). Pyruvate formate-lyase reaction in Escherichia coli, Eur. J. Biochem. 11, 316-327.
19. Lin, E. C. C., and Iuchi, S. (1994). Role of protein phosphorylation in the regulation of aerobic metabolism by the Arc system in Escherichia coli. In "Phosphate in Microorganisms: Cellular and Molecular Biology" (A. Torriani-Gorini, E. Yagil, and S. Silver, Eds.), pp. 290-295, American Society for Microbiology, Washington, DC.
20. Lynch, A. S., and Lin, E. C. C. (1996). Responses to molecular oxygen. In "Escherichia coli and Salmonella: Cellular and Molecular Biology" (F. Neidhardt, R. Curtis III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasnik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger, Eds.), pp. 1526-1538, American Society for Microbiology, Washington, DC.
21. Mat-Jan, F., Alam, K. Y., and Clark, D. P. (1989). Mutants of Escherichia coli deficient in the fermentative lactate dehydrogenase, J. Bacteriol. 171, 342-348.
22. Pascal, M. C., Chippaux, M., Jaoudé, A. A., Blaschkowski, H. P., and Knappe, J. (1981). Mutants of Escherichia coli K12 with defects in anaerobic pyruvate metabolism, J. Gen. Microbiol. 124, 35-42.
23. Pecher, A., Zinoni, F., Jatisatienr, C., Writh, R., Hennecke, H., and Böck, A. (1983). On the redox control of synthesis of anaerobically induced enzymes in enterobacteriaceae, Arch. Microbiol. 136, 131-136.
24. Rose, R. E. (1988). The nucleotide sequence of pACYC184, Nucleir Acids Res. 16, 355.
25. Sawers, G. (1993). Specific transcriptional requirements for positive regulation of the anaerobically inducible pfl operon by ArcA and FNR, Mol. Microbiol. 10, 737-747.
26. Sawers, G., and Böck, A. (1988). Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12, J. Bacteriol. 170, 5330-5336.
27. Sawers, G., and Böck, A. (1989). Novel transcriptional control of the pyruvate formate-lyase gene: Upstream regulatory sequences and multiple promoters regulate anaerobic expression, J. Bacteriol. 171, 2485-2498.
28. Sawers, G., and Suppman, B. (1992). Anaerobic induction of pyruvate formate-lyase gene expression is mediated by the ArcA and FNR proteins, J. Bacteriol. 174, 3474-3478.
29. 70-dependent transcription in vitro and heterologous expression in Pseudomonas putida in vivo, J. Bacteriol. 171, 4930-4937.
30. Small, J. R., and Kacser, H. (1993). Responses of metabolic systems to large changes in enzyme activities and effectors. 1. The linear treatment for unbranched, Eur. J. Biochem. 42, 613-624.
31. Stock, J. B., Rauch, B., and Roseman, S. (1977). Periplasmic space in Salmonella typhimurium and Escherichia coli, J. Biol. Chem. 252, 7850-7861.
32. Tarmy, E. M., and Kaplan, N. O. (1968a). Chemical characterization of D-lactate dehydrogenase from Escherichia coli B, J. Biol. Chem. 243, 2579-2586.
33. Tarmy, E. M., and Kaplan, N. O. (1968b). Kinetics of Escherichia coli B D-lactate dehydrogenase and evidence for pyruvate-controlled change in conformation, J. Biol. Chem. 243, 2587-2596.
34. Tolentino, G. J., Meng, S.-Y., Bennett, G. N., and San, K.-Y. (1992). A pH-regulated promoter for the expression of recombinant proteins in Escherichia coli, Biotechnol. Lett. 14, 157-162.
35. Winkler, H. H., and Wilson, T. H. (1966). The role of energy coupling in the transport of beta-galactosides by Escherichia coli, J. Biol. Chem. (HIV) 241, 2200-2211.
36. Yang, Y.-T., Aristidou, A. A., San, K.-Y., and Bennett, G. N. (1999). Metabolic flux analysis of E. coli deficient in the acetate production pathway and expressing the B. subtilis acetolactate synthase, Metab. Eng. 1, 26-34.
37. Yee, L., and Blanch, H. W. (1993), Recombinant trypsin troduction in high cell density fed-batch cultures of Escherichia coli, Biotechnol. Bioeng. 41, 781-790.