Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli

Microbiology

Volumn 143, Issue 3, 1997, Pages 775-783

  Kaiser, Manuela ^a   Sawers, Gary ^b  

^a UNIVERSITY OF MUNICH   (Germany)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

Anaerobic metabolism; Fnr and ArcA; Gene regulation; Overlapping promoters; Transcription factors

Indexed keywords

FUMARIC ACID; NITRATE; RNA POLYMERASE;

AEROBIC BACTERIUM; ANAEROBIC BACTERIUM; ARTICLE; BINDING COMPETITION; ESCHERICHIA COLI; GENE FUSION; MUTATION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; REDUCTION; RESPIRATION CONTROL; TRANSACTIVATION;

EID: 0030899580     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-3-775     Document Type: Article

References (23)

1. Adhya, S. (1987). The galactose operon. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology, pp. 1503-1512. Edited by F. C. Neidhardt, J. L. Ingraham, K. Brooks Low, B. Magasanik, M. Schaechter & H. E. Umbarger. Washington, DC: American Society for Microbiology.
2. Barrios, H., Fischer, H.-M., Hennecke, H. & Morett, E. (1995). Overlapping promoters for two different RNA polymerase holoenzymes control Bradyrhizobium japonicum nif A expression. J Bacteriol 177, 1760-1765.
3. Begg, Y. A., Whyte, J. A. & Haddock, B. A. (1977). The identification of mutants of Escherichia coli deficient in formate dehydrogenase and nitrate reductase activites using dye indicator plates. FEMS Microbiol Lett 2, 47-50.
4. Bingham, A. H. A., Ponnambalam, S., Chan, B. & Busby, S. (1986). Mutations that reduce expression from the P2 promoter of the Escherichia coli galactose operon. Gene 41, 67-74.
5. Birkmann, A., Sawers, R. G. & Böck, A. (1987). Involvement of the ntrA gene product in the anaerobic metabolism of Escherichia coli. Mol Gen Genet 210, 535-542.
6. Casadaban, M. J. & Cohen, S. N. (1979). Lactose genes fused to exogenous promoters in one step using Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci USA 76, 4530-4533.
7. Compan, I. & Touati, D. (1994). Anaerobic activation of arcA transcription in Escherichia coli: roles of Fnr and ArcA. Mol Microbiol 11, 955-964.
8. Drapai, N. & Sawers, G. (1995a). Purification of ArcA and analysis of its specific interaction with the pfl promoter-regulatory region. Mol Microbiol 16, 597-607.
9. Drapai, N. & Sawers, G. (1995b). Promoter 7 of the Escherichia coli pfl operon is a major determinant in the anaerobic regulation of expression by ArcA. J Bacteriol 111, 5338-5341.
10. Fu, H. A., Iuchi, S. & Lin, E. C. C. (1991). The requirement of ArcA and Fnr for peak expression of the cyd operon in Escherichia coli under micro-aerobic conditions. Mol Gen Genet 226, 209-213.
11. Irani, M., Musso, R. & Adhya, S. (1989). Cyclic-AMP-dependent switch in initiation of transcription from the two promoters of the Escherichia coli gal operon: identification and assay of 5′-triphosphate ends of mRNA by GTP: RNA guanyltransferase. J Bacteriol 171, 1623-1630.
12. Kaiser, M. & Sawers, G. (1995). Fnr activates transcription from the P6 promoter of the pfl operon in vitro. Mol Microbiol 18, 331-342.
13. Knappe, J. & Sawers, G. (1990). A radical-chemical route to acetyl-CoA: the anaerobically induced pyruvate formate-lyase system of Escherichia coli. FEMS Microbiol Rev 75, 383-398.
14. Miller, J. H. (1972). Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
15. Sawers, G. (1993). Specific transcriptional requirements for positive regulation of the anaerobically inducible pfl operon by ArcA and FNR. Mol Microbiol 10, 737-747.
16. Sawers, G. & Böck, A. (1988). Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12. J Bacteriol 170, 5330-5336.
17. Sawers, G. & Bock, A. (1989). Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J Bacteriol 171, 2485-2498.
18. Sawers, G. & Suppmann, B. (1992). Anaerobic induction of pyruvate formate-lyase gene expression is mediated by the ArcA and FNR proteins. J Bacteriol 174, 3474-3478.
19. Simons, R. W., Houman, F. & Kleckner, N. (1987). Improved single and multicopy lac-based protein and operon fusion cloning tools. Gene 53, 85-96.
20. Sirko, A., Zehelein, E., Freundlich, M. & Sawers, G. (1993). Integration host factor is required for anaerobic pyruvate induction of pfl operon expression in Escherichia coli. J Bacteriol 175, 5769-5777.
21. Spiro, S. & Guest, J. R. (1990). FNR and its role in oxygen-regulated gene expression in Escherichia coli. FEMS Microbiol Rev 75, 399-428.
22. Suppmann, B. & Sawers, G. (1994). Isolation and characterisation of hypophosphite-resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol 11, 965-982.
23. Tseng, C.-P., Albrecht, J. & Gunsalus, R. P. (1996). Effect of microaerophilic cell growth conditions on expression of the aerobic (cyoABCDE and cydAB) and anaerobic (narGHJI, frdABCD and dmsABC) respiratory pathway genes in Escherichia coli. J Bacteriol 178, 1094-1098.