The sea urchin sns insulator blocks CMV enhancer following integration in human cells

Biochemical and Biophysical Research Communications

Volumn 284, Issue 4, 2001, Pages 987-992

  Di Simone, Paola ^a   Di Leonardo, Aldo ^a   Costanzo, Giorgia ^a   Melfi, Raffaella ^a   Spinelli, Giovanni ^a  

^a UNIVERSITY OF PALERMO   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CIS ACTING ELEMENT; GREEN FLUORESCENT PROTEIN; PROTEIN TYROSINE KINASE;

ARTICLE; BINDING SITE; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA INTEGRATION; ENHANCER REGION; EXPRESSION VECTOR; GENE EXPRESSION; GENE FUNCTION; GENE REARRANGEMENT; GENE TRANSFER; HUMAN; HUMAN CELL; INSULATOR GENE; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; RIBONUCLEASE PROTECTION ASSAY; SEA URCHIN; TRANSGENE;

EID: 0034815582     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5082     Document Type: Article

References (22)

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3. Insulators and boundaries: Versatile regulatory elements in the eukaryotic genome
4. Putting boundaries on silence
5. A position-effect assay for boundaries of higher order chromosomal domains
6. A group of scs elements function as domain boundaries in an enhancer-blocking assay
7. DNA position-specific repression of transcription by a Drosophila zinc finger protein
8. Modulation of enhancer-promoter interactions by insulators in the Drosophila embryo
9. Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes
10. A 5′ element of the chicken β-globin domain serves as an insulator in human erythroid cells and protects against position effects in Drosophila
11. The protein CTCF is required for the enhancer blocking activity of vertebrate insulators
12. Positional enhancer-blocking activity of the chicken β-globin insulator in transiently transfected cells
13. Methylation of a CTCF-dependent boundary controls imprinted expression of the Igf2 gene
14. CTCF mediates methylation-sensitive enhancer-blocking activity at the H19/Igf2 locus
15. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators
16. Ligand-inducible and liver-specific target gene expression in transgenic mice
17. A chromatin insulator protects retrovirus vectors from chromosomal position effects
18. The cHS4 insulator increases the probability of retroviral expression at random chromosomal integration sites
19. Insulation from viral transcriptional regulatory elements improves inducible transgene expression from adenovirus vectors in vitro and in vivo
20. Enhancer blocking activity located near the 3′ end of the sea urchin early H2A histone gene
21. Functional characterization of the enhancer blocking element of the sea urchin early histone gene cluster reveals insulator properties and three essential cis-acting sequences
22. Modulator factor-binding sequence of the sea urchin early histone H2A promoter acts as an enhancer element