On the preparation, characterization, and enzymatic activity of fungal protease-gold colloid bioconjugates

Bioconjugate Chemistry

Volumn 12, Issue 5, 2001, Pages 684-690

  Gole, A ^a   Dash, C ^a   Soman, C ^a   Sainkar, S R ^a   Rao, M ^a   Sastry, M ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

COLLOIDS; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; GOLD NANOPARTICLES; METAL NANOPARTICLES; SCANNING ELECTRON MICROSCOPY; ULTRAVIOLET VISIBLE SPECTROSCOPY; X RAY DIFFRACTION;

ACTIVITY MEASUREMENTS; ASPARTIC PROTEASE; BIOCATALYTIC ACTIVITY; BIOCONJUGATES; COLLOIDAL GOLD; ENZYMATIC ACTIVITIES; GOLD COLLOID; PROTEIN MOLECULES; PROTEIN SOLUTION; SIMPLE++;

BIOMOLECULES;

ASPERGILLUS; ASPERGILLUS SAITOI; FUNGI;

EID: 0034814675     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc0001241     Document Type: Article

References (43)

1. Enhanced N-dimethylase activity of cytochrome c bound to a phosphate-bearing synthetic bilayer membrane
2. Phase-separated two-component self-assembled organosilane monolayers and their use in selective adsorption of a protein
3. Plasmon resonance permits in-situ measurement of protein adsorption on self-assembled monolayer of alkanethiolates on gold
4. Electron-transfer properties of cytochrome c Langmuir-Blodgett films and interactions of cytochrome c with lipids
5. Thermal stability of protein secondary structure in Langmuir-Blodgett films
6. Activity and stability of enzymes incorporated into acrylic polymers
7. Electrostatic immobilization of glucose oxidase in a weak acid, polyelectrolyte hyperbranched ultrathin film on gold: Fabrication characterization, and enzymatic activity
8. Protein modified electrodes. The glucose oxidase/poly pyrole systems
9. Lipase-silicone biocomposites: Efficient and versatile immobilized biocatalysts
10. Nanoencapsulation of cytochrome c and horseradish peroxidase at the galleries of α-zirconium phosphate
11. Blue-print for a lipase support: Use of hydrophobic controlled-pore glasses as model systems
12. Encapsulation and biocatalytic activity of the enzyme pepsin in fatty lipid films by selective electrostatic interactions
13. Preparation, characterization and enzymatic activity of encapsulated fungal protease-fatty lipid biocomposite films
14. Assembly of alternating polyelectrolyte and protein multilayer films for immunosensing
15. QCM study of DNA immobilization and hybridization for nucleic acid sensor development
16. Protein multilayer formation on colloids through a stepwise self-assembly technique
17. Preparation of enzyme multilayers on colloids for biocatalysis
18. Immunomicrospheres: Reagents for cell labeling and separation
19. How proteins stabilize colloidal particles by means of hydration forces
20. Colloidal particles as immunodiagnostics: Preparation and FFF characterization
21. Experimental evidence regarding the influence of surface charge on the bridging flocculation mechanism
22. The adsorption of bovine serum albumin on positively and negatively charged polystyrene latices
23. Protein: Colloid conjugates for surface enhanced raman scattering: Stability and control of protein orientation
24. Heightened electromagnetic fields between metal nanoparticles: Surface enhanced raman scattering from metal-Cytochrome c-metal sandwiches
25. Orientation of Cytochrome c adsorbed on a citrate-reduced silver colloid surface
26. Spectroscopy of single hemoglobin molecules by surface enhanced raman spectroscopy
27. Comparison of colloidal gold electrode fabrication methods: The preparation of a horseradish peroxidase enzyme electrode
28. A xanthine oxidase/colloidal gold enzyme electrode for amperometric biosensor applications
29. Colloidal gold as a biocompatible immobilization matrix suitable for the fabrication of enzyme electrodes by electrodeposition
30. Role of particle size in individual and competitive diffusion of carboxylic acid derivatized colloidal gold particles in thermally evaporated fatty amine films
31. Optical absorption spectra of nanocrystal gold molecules
32. Quantitation of protein
33. Fluorescence quenching studies with proteins
34. The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin
35. An optical absorption study of the biotinavidin interaction on colloidal silver and gold particles
36. A gold nanoparticle/latex microsphere-based colorimetric oligonucleotide detection method
37. Programming the assembly of two- and three-dimensional architectures with dna and nanoscale inorganic building blocks
38. What controls the optical properties of dna-linked gold nanoparticle assemblies?
39. Two-dimensional arrangement of a functional protein by cysteine-gold interaction: Enzyme activity and characterization of a protein monolayer on a gold substrate
40. Synthesis and characterization of hydrophobic, organically soluble gold nanocrystals functionalized with primary amines
41. Characterization of polyelectrolyteprotein multilayer films by atomic force microscopy, scanning electron microscopy, and fourier transform infrared reflection-absorption spectroscopy
42. Thermodynamic control of gold nanocrystal size: Experiment and theory