Lipase-silicone biocomposites: Efficient and versatile immobilized biocatalysts

Journal of the American Chemical Society

Volumn 121, Issue 41, 1999, Pages 9487-9496

  Gill, Iqbal ^a,b   Pastor, Eitel ^a   Ballesteros, Antonio ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^b HOFFMANN LA ROCHE INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SILICONE; TRIACYLGLYCEROL LIPASE;

ADSORPTION; ARTICLE; CATALYST; HYDROLYSIS; IMMOBILIZATION; ORGANIC CHEMISTRY; PARTICULATE MATTER; PHYSICAL CHEMISTRY; REACTION ANALYSIS; TECHNOLOGY;

EID: 0032706857     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9924849     Document Type: Article

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65. 12 or on the large scale more conveniently by transfer dehydrogenation with acetone, using Zn(II)/Sn(II) as catalysts.
66. -1 in 0.1 or 0.2 M metaphosphate buffer, pH 7, containing 20 mM calcium acetate, 5 °C), at an applied protein load of 10% w/w of PHOMS, gave the following activity immobilizations: papain, 96%; thermolysin, >99%; almond β-D-glucosidase, 98%; C. rugosa lipase-B, >99%; S. chromofuscus phospholipase D, 98%. Adsorption took 10-30 min, and less than 3% leaching of protein/activity was detected upon washing (3 × 10-fold volumes of buffer, 5 °C, 1 h).
67. -1.
68. The silanol content of PHOMS varied between 6 and 12.3 mmol/g (46-93% of theoretical maximum), depending upon the mode of preparation and the aging and drying conditions. Wet processing at low pH and/or high temperature, and/or dry calcination affords a low-silanol/high-siloxane PHOMS. Conversely, low temperature and/or high pH treatment afford highly silanolated products. Silanol coverage was determined by titration with LAH, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC).
69. 18 This condensation is catalyzed by Sn(II), Fe(III), Ti(IV), Zn(II), Pt(IV), and other metal salts.
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73. -1.
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82. We have routinely fabricated lipase-PHOMS-silicone catalysts on a multikilo scale, and have estimated the costs of producing monoliths, granulates, coated particulates, and foams to be in the range of $20-35 per kilo of composite. The prices of commercial food and catalyst grade lipases range over $100-2000 per kilo. For medium to high catalytic density immobilizates (5-15% w/w lipase loading), the additional cost of immobilization is about 9-30%. Considering that the activities and long-term stabilities of the composites can exceed those of the native enzymes by 1-50-fold and 10-30-fold respectively, leading to performance improvements of 10-1500-fold, it can be seen that the economics are rather attractive.