A high-efficiency synthetic promoter that drives transgene expression selectively in noradrenergic neurons

Human Gene Therapy

Volumn 12, Issue 14, 2001, Pages 1731-1740

  Hwang, D Y ^a   Carlezon, Jr ^a   Isacson, O ^a   Kim, K S ^a  

^a MCLEAN HOSPITAL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DOPAMINE BETA MONOOXYGENASE; VIRUS VECTOR;

ADENOVIRUS; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; CELL COUNT; CELL LINE; CELL SPECIFICITY; CEREBELLUM; CONTROLLED STUDY; DENTATE GYRUS; GENE EXPRESSION SYSTEM; HUMAN; HUMAN CELL; LOCUS CERULEUS; MICROINJECTION; NONHUMAN; NORADRENERGIC NERVE; PROMOTER REGION; PROTEIN MOTIF; RAT; REPORTER GENE; TATA BOX; TRANSGENE;

ADENOVIRIDAE; ANIMALS; BASE SEQUENCE; BETA-GALACTOSIDASE; BRAIN; CELL LINE; DOPAMINE BETA-HYDROXYLASE; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENES, REPORTER; GENETIC VECTORS; HELA CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NEURONS; PLASMIDS; PROMOTER REGIONS (GENETICS); RATS; RECEPTORS, ADRENERGIC; TIME FACTORS; TRANSCRIPTION, GENETIC; TRANSGENES; TUMOR CELLS, CULTURED;

EID: 0034808550     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/104303401750476230     Document Type: Article

References (36)

1. Transfer of a foreign gene into the brain using adenovirus vectors
2. The pathogenesis of depression; reconsideration of neurotransmitter data
3. Norepinephrine in depressive reactions
4. Sensitization to morphine induced by viral-mediated gene transfer
5. Gene therapy in the CNS
6. A model system for in vivo gene transfer into the central nervous system using an adenoviral vector
7. Towards gene therapy for the central nervous system
8. A simplified system for generating recombinant adenoviruses
9. Promoters for expression of gene products within neurons and glia
10. Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements
11. Neuron-specific expression of the human dopamine beta-hydroxylase gene requires both the cAMP-response element and a silencer region
12. Identification of a negative regulatory element in the 5′-flanking region of the human dopamine beta-hydroxylase gene
13. Noradrenergic-specific transcription of the dopamine beta-hydroxylase gene requires synergy of multiple cis-acting elements including at least two Phox2a-binding sites
14. The cAMP-dependent protein kinase regulates transcription of the dopamine beta-hydroxylase gene
15. Functional and high level expression of human dopamine beta-hydroxylase in transgenic mice
16. Analysis of the human dopamine beta-hydroxylase promoter: Transcriptional induction by cyclic AMP
17. An adenovirus vector for gene transfer into neurons and glia in the brain
18. Gene therapy - Promise, pitfalls, and prognosis
19. Differential and persistent expression patterns of CNS gene transfer by an adeno-associated virus (AAV) vector
20. The dopamine beta-hydroxylase gene promoter directs expression of E. coli lacZ to sympathetic and other neurons in adult transgenic mice
21. Defects in sensory and autonomic ganglia and absence of locus coeruleus in mice deficient for the homeobox gene Phox2a
22. Under siege: The brain on opiates
23. Genetically modified skin fibroblasts persist long after transplantation but gradually inactivate introduced genes
24. Neuronal-specific gene expression - The interaction of both positive and negative transcriptional regulators
25. Dopamine beta-hydroxylase deficiency. A genetic disorder of cardiovascular regulation
26. Long-term in vivo expression of retrovirus-mediated gene transfer in mouse fibroblast implants
27. Multiple protein factors interact with the cis-regulatory elements of the proximal promoter in a cell-specific manner and regulate transcription of the dopamine beta-hydroxylase gene
28. An HSV-1 vector containing the rat tyrosine hydroxylase promoter enhances both long-term and cell type-specific expression in the midbrain
29. The expression pattern of the transcription factor Phox2 delineates synaptic pathways of the autonomic nervous system
30. The mouse homeodomain protein Phox2 regulates Ncam promoter activity in concert with Cux/CDP and is a putative determinant of neurotransmitter phenotype
31. Genome and gene therapy
32. Gene therapy - Promises, problems and prospects
33. Diverse stabilities of expression in the rat brain from different cellular promoters in a helper virus-free herpes simplex virus type 1 vector system
34. Paired-like homeodomain proteins, Phox2a and Phox2b, are responsible for noradrenergic cell-specific transcription of the dopamine beta-hydroxylase gene