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note Academic, Sydney, Australia
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Rats were anesthetized (65 mg/kg sodium pentobarbital, intraperitoneal) and given atropine (0.25 mg/kg, s.c.) to minimize bronchial secretions. Unilateral microinjections (2.0 μl) of recombinants were delivered over 10 min into the VTA {relative to bregma: AP = -5.3, lat. = ±2.0, DV = 7.6 mm below dura [G. Paxinos and C. Watson, The Rat Brain in Strereotaxic Coordinates (Academic, Sydney, Australia, 1986)]}. The injection syringe was angled at 10° from the midline. For sham surgery, the injection syringe was lowered 1 mm into the brain. Rats were retained if the microinjection site was within the VTA.
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The Rat Brain in Strereotaxic Coordinates
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Paxinos, G.1
Watson, C.2
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1842376440
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note
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7 infectious units/ml; titers did not differ by more than 10% among preparations. Transgene expression was regulated by the constitutive promoter for the HSV immediate-early gene IE 4/5.
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19
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1842291681
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note
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VTAs were dissected from the injected hemisphere 4 days after vector microinjection; immunoblotting was done as described (5).
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20
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0028172887
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note
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Rats were overdosed with sodium pentobarbital and perfused with 0.9% saline followed by 4% paraformaldehyde; brains were kept overnight in 20% glycerol before slicing (40 μm, or 20 μm for double-labeling). β-Galactosidase expression was examined as described [N. Min, T. H. Joh, K. S. Kim, C. Peng, J. H. Son, Mol. Brain Res. 27, 281 (1994)].
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Min, N.1
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Immunohistochemical analyses for double-labeling of GluR1 and tyrosine hydroxylase were similar to those described [M. T. Berhow, N. Hiroi, L. A. Kobierski, S. E. Hyman, E. J. Nestler, J. Neurosci. 16, 8019 (1996)].
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1842404942
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note
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Immunohistochemical analysis for GluR2 (Chemicon GluR2/3; 1: 500) was similar to that described for GluR1 (76).
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J. M. Liebman and S. J. Cooper, Eds. (Oxford Univ. Press, Oxford)
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1842373443
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note
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Supported by grants (to E.J.N.) and a fellowship (to W.A.C.) from the National Institute on Drug Abuse (USA), and grants (to R.G.K. and R.L.N.) from the National Institutes of Health. We thank N. Hiroi for his contributions.
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