Interference of cytokeratin-20 and mammaglobin-reverse-transcriptase polymerase chain assays designed for the detection of disseminated cancer cells

Medical Oncology

Volumn 18, Issue 1, 2001, Pages 33-38

  Krüger, William H ^a,b   Jung, Roman ^a   Detlefsen, Birte ^a   Mumme, Sandra ^a   Badbaran, Anita ^a   Brandner, Johanna ^a   Renges, Helmut ^a   Kröger, Nicolaus ^a   Zander, Axel R ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

Cytokeratin 20; Disseminated tumor cells; Epithelial cancer; Gene expression; Mammaglobin; Reverse transcriptase polymerase chain reaction

Indexed keywords

CYTOKERATIN 20; CYTOKINE; GLOBIN; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; MAMMAGLOBIN; UNCLASSIFIED DRUG;

ARTICLE; CANCER CELL; CANCER DIAGNOSIS; CELL STIMULATION; CONTROLLED STUDY; CYTOKINE PRODUCTION; DISSEMINATED CANCER; EPITHELIUM CELL; GENE AMPLIFICATION; GENETIC TRANSCRIPTION; GRANULOCYTE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CARCINOMA; DNA, NEOPLASM; FALSE POSITIVE REACTIONS; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; INTERMEDIATE FILAMENT PROTEINS; KERATIN-20; NEOPLASM METASTASIS; NEOPLASM PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRANSCRIPTION, GENETIC; TUMOR CELLS, CULTURED; UTEROGLOBIN;

EID: 0034807186     PISSN: 13570560     EISSN: None     Source Type: Journal    
DOI: 10.1385/MO:18:1:33     Document Type: Article

References (14)

1. Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction
2. Diagnosis of micrometastases by the amplification of tissue-specific genes
3. Reverse transcriptase/polymerase chain reaction detection of cytokeratin-19 mRNA in bone marrow and blood of breast cancer patients
4. Detection of circulating mammary carcinoma cells in the peripheral blood of breast cancer patients via a nested reverse transcriptase polymerase chain reaction assay for mammaglobin mRNA
5. Limitations of reverse-transcriptase polymerase chain reaction analyses for detection of micrometastatic epithelial cancer cells in bone marrow
6. Specificity of reverse transcriptase polymerase chain reaction assays designed for the detection of circulating cancer cells is influenced by cytokines in vivo and in vitro
7. Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granuloctes
8. Expression of human milk fat globulin proteins in cells of haemopoietic origin
9. MUC1 expression in hemopoietic tissues
10. Sensitive and specific cytokeratin 18 reverse transcription-polymerase chain reaction that excludes amplification of processed pseudogenes from contaminating genomic DNA
11. Comparative analysis of bone marrow and venous blood isolates from gastrointestinal cancer patients for the detection of disseminated tumor cells using reverse transcription PCR
12. Sensitivity of assays designed for the detection of disseminated epithelial tumor cells is influenced by cell separation methods
13. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
14. Mammaglobin expression in primary, metastatic, and occult breast cancer