A transgene with repeated DNA causes high frequency, post-transcriptional suppression of ACC-oxidase gene expression in tomato

Plant Journal

Volumn 15, Issue 6, 1998, Pages 737-746

  Hamilton, Andrew J ^a,b   Brown, Stephen ^a,c   Yuanhai, Man ^a,d   Ishizuka, Masakatsu ^a,e   Lowe, Alex ^a   Solis, Angel Gabriel Alpuche ^a   Grierson, Don ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^b SAINSBURY LABORATORY   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d UNIVERSITY OF BATH   (United Kingdom)

^e SHIONOGI AND CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

1 AMINOCYCLOPROPANE 1 CARBOXYLIC ACID OXIDASE; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; PLANT GENETICS; TOMATO; TRANSGENE; TRANSGENIC PLANT;

EID: 0031712336     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.1998.00251.x     Document Type: Article

References (55)

1. Angel, S.M. and Baulcombe, D.C. (1997) Consistent gene silencing in transgenic plants expressing a replicating potato virus X RNA. Embo. J. 16, 3675-3684.
2. Assad, F.F., Tucker, K.L. and Signer, E.R. (1993) Epigenetic repeat induced gene silencing (RIGS) in Arabidopsis. Plant Mol. Biol. 22, 1067-1085.
3. Barry, C.S., Blume, B., Bouzayen, M., Cooper, W., Hamilton, A.J. and Grierson, D. (1996) Differential expression of the 1-aminocyclopropane-1-carboxylate oxidase gene family of tomato. Plant J. 9, 525-535.
4. Baulcombe, D.C. and English, J.J. (1996) Ectopic pairing of homologous DNA and post-transcriptional gene silencing in transgenic plants. Current Opinion Biotech. 7, 173-180.
5. Bevan, M. (1984) Binary Agrobacterium vectors for plant transformation. Nucl. Acids Res. 24, 8711-8721.
6. Brusslan, J.A., Karlin-Neumann, G.A., Huang, L. and Tobin, E.M. (1993) An Arabidopsis mutant with a reduced level of cab140 mRNA is a result of co-suppression. Plant Cell. 5, 667-677.
7. deCarvalho, F., Gheysen, G., Kushnir, S., van Montagu, M., Inzé, D. and Castresana, C. (1992) Co-suppression of β-1,3-glucanase transgene expression in homozygous plants. Embo J. 11, 2595-2602.
8. deCarvalho-Niebel, F., Frendo, P., Van Montagu, M. and Cornelissen, M. (1995) Post-transcriptional co-suppression of β-1,3-glucanase genes does not affect accumulation of transgene nuclear mRNA. Plant Cell. 7, 347-358.
9. Covey, S.N., AI-Kaff, N.S., Langara, A. and Turner, D.S. (1997) Plants combat infection by gene silencing. Nature, 385, 781-782.
10. Dawson, W.O. (1996) Gene silencing and virus resistance: a common mechanism. Trends Plant Sci. 1, 107-108.
11. Dehio, C. and Schell, J. (1994) Identification of plant genetic loci involved in a post-transcriptional mechanism for meiotically reversible transgene silencing. Proc. Natl Acad. Sci. USA, 91, 5538-5542.
12. Dougherty, W.G. and Parks, T.D. (1995) Transgenes and gene suppression: telling us something new? Curr. Opin. Cell Biol. 7, 399-405.
13. Elmayan, T. and Vaucheret, H. (1996) Expression of single copies of a strongly-expressed 35S-transgene can be silenced post-transcriptionally. Plant J. 9, 787-797.
14. English, J.J., Mueller, E. and Baulcombe, D.C. (1996) Suppression of virus accumulation in transgenic plants exhibiting silencing of nuclear genes. Plant Cell. 8, 179-188.
15. Finnegan, J. and McElroy, D. (1994) Transgene inactivation: plants fight backl. Bio/Technology, 12, 883-888.
16. Goodwin, J., Chapman, K., Swaney, S., Parks, D., Wernsman, E.A. and Dougherty, W.G. (1996) Genetic and biochemical dissection of transgenic RNA-mediated virus -resistance. Plant Cell, 8, 95-105.
17. Goring, D.R., Thomson, L and Rothstein, S.J. (1991) Transformation of a partial nopaline synthase gene into tobacco suppresses the expression of a resident wild type gene. Proc. Natl Acad. Sci. USA. 88, 1770-1774.
18. Green, P.J. (1993) Control of mRNA stability in plants. Plant Physiol. 102, 1065-1070.
19. Grierson, D., Fray, R.G., Hamilton, A.J., Smith, C.J.S. and Watson, C.F. (1991) Does co-suppression of sense genes in transgenic plants involve anti-sense RNA? Trends Biotechnol. 9, 122-123.
20. Hamilton, A.J., Bouzayen, M. and Grierson, D. (1991) Identification of a tomato gene for the ethylene-forming enzyme by expression in yeast. Proc. Natl Acad. Sci. USA. 88, 7434-7437.
21. Hamilton, A.J., Lycett, G.W. and Grierson, D. (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature, 346, 284-287.
22. Hobbs, S.L.A., Warkentin, T.D. and DeLong, C.M.O. (1993) Transgene copy number can be positively or negatively associated with transgene expression. Plant Mol. Biol. 21, 17-26.
23. Holdsworth, M.J., Bird, C.R., Ray, J., Schuch, W. and Grierson, D. (1987) Structure and expression of an ethylene-related messenger-RNA from tomato. Nuc. Acids Res. 15, 731-739.
24. Inglebrecht, I., van Houdt, H., van Montagu, M. and Depicker, A. (1994) Post-transcriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. Proc. Natl Acad. Sci. USA 91, 10502-10506.
25. Jiang, C.-Z., Kliebenstein, D., Ke, N. and Rodermel, S. (1994) Destabilisation of rbcS sense transcripts by antisense RNA. Plant Mol. Biol. 25, 569-576.
26. Jorgensen, R. (1396) Chalcone synthase co-suppression phenotypes in petunia flowers: comparison of sense vs. antisense constructs and single-copy vs. complex T-DNA sequences. Plant Mol. Biol. 31, 957-953.
27. Kozak, M. (1986) Influence of mRNA secondary structure on initiation by eukaryotic ribosomes. Proc. Natl Acad. Sci. USA. 83, 2850-2854.
28. Kumagai, M.H., Donson, J., Dellacioppa, G. and Harvey, D. (1995) Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA. Proc. Natl Acad. Sci. USA. 92, 1679-1683.
29. Li, Y., Strabala, T.J., Hagen, G. and Guilfoyle, T.J. (1994) The soybean SAUR open reading frame contains a cis element responsible for cycloheximide-induced mRNA accumulation. Plant Mol. Biol. 24, 715-723.
30. Lindbo, J.A., Silva-Rosales, L., Proebsting, W.M. and Dougherty, W.G. (1993). Induction of a highly specific anti-viral state in transgenic plants: Implications for gene regulation and virus resistance. Plant Cell, 5, 1749-1759.
31. Linn, F., Heidmann, I., Saedler, H. and Meyer, P. (1990) Epigenetic changes in the expression of the maize A1 gene in Petunia hybrids. Role of numbers of integrated gene copies and state of methylation. Mol. Gen. Genet 222, 329-336.
32. Lohuis, M.T., Muller, A., Heidemann, I., Niedenhof, I. and Meyer, P. (1995) A repetitive DNA fragment carrying a ho spot for de novo DMA methylation enhances expression variegation in tobacco and petunia. Plant J. 8, 919-932.
33. Matzke, M.A. and Matzke, A.J.M. (1993) Genomic imprinting in plants: parental effects and trans-inactivation phenomena. Annu. Rev. Plant Physiol. Plant Mol. Biol. 44, 53-76.
34. Matzke, M.A. and Matzke, A.J.M. (1995) How and why do plants inactivate homologous (trans) genes. Plant Physiol. 107, 679-685.
35. Matzke, M.A., Matzke, A.J.M. and Eggleston, W.B. (1996) Paramutation and transgene silencing: a common response to invasive DNA? Trends Plant Sci. 1, 382-388.
36. Meins, F., Jr and Kunz, C. (1994) Silencing of chitinase expression in transgenic plants: an autoregulated model. In: Homologous Recombination and Gene Silencing in Plants (Paszkowski, J. ed.). Dordrecht: Kluwer Academic Publishers, pp. 335-348.
37. Metzlaff, M., O'Dell, M., Cluster, P.D. and Flavell, R.B. (1997) RNA-mediated RNA degradation and chalcone synthase A silencing in Petunia. Cell, 88, 845-854.
38. Meyer, P., Heidmann, I. and Niedenhof, I. (1993) Differences in DNA-methylation are associated with a paramutation phenomenon in transgenic petunia. Plant J. 4, 89-100.
39. Meyer, P. and Saedler, H. (1996) Homology dependent gene silencing in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47, 23-48.
40. Mittlesten Scheid, O., Paszkowski, J. and Potrykus, I. (1991) Reversible inactivation of a transgene in Arabidopsis thaliana. Mol. Gen. Genet. 228, 104-112.
41. Mol, J.N.M., Stuitje, A.R. and van der Krol, A. (1989) Genetic manipulation of floral pigmentation genes. Plant Mol. Biol. 13, 287-294.
42. Neuhuber, F., Park, Y.-D., Matzke, A.J.M. and Matzke, M.A. (1994) Susceptibility of transgene loci to homology-dependent gene silencing. Mol. Gen. Genet. 244, 230-241.
43. Que, Q., Wang, H.-Y., English, J.J. and Jorgensen, R.A. (1997) The frequency and degree of co-suppression by sense chalcone synthase transgenes are dependent on transgene promoter strength and are reduced by premature nonsense codons in the transgene coding sequence. Plant Cell, 9, 1357-1368.
44. Ross, J. (1995) mRNA stability in mammalian Cells. Microbiol. Rev. 59, 16-95.
45. Schilling, L.J. and Farnham, P.J. (1994) Inappropriate transcription from the 5′ end of the murine dihydrofolate-reductase gene masks transcriptional regulation. Nuc. Acids Res. 22, 3061-3068.
46. Sensel, M.G., Binder, R., Lazier, C.B. and Williams, D.L (1994) Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14, 1733-1742.
47. Seymour, G.B., Fray, R.G., Hill, P. and Tucker, G.A. (1993) Down-regulation of two non-homologous endogenous tomato genes with a single chimaeric sense gene construct. Plant Mol. Biol. 23, 1-9.
48. Sijen, T., Wellink, J., Hiriart, J.-B. and van Kammen, A. (1996) RNA-mediated virus resistance: role of repeated transgenes and delineation of target regions. Plant Cell, 8, 2277-2294.
49. Smith, H.A., Powers, H., Swaney, S., Brown, C. and Dougherty, W.G. (1995) Transgenic potato virus Y resistance in potato: evidence for an RNA-mediated cellular response. Phytopathology, 85, 864-870.
50. Smith, C.J.S., Slater, A. and Grierson. D. (1986) Rapid appearance of an mRNA correlated with ethylene synthesis encoding a protein of molecular weight 35 000. Planta, 168, 94-100.
51. Smith, C.J.S., Watson, C.F., Bird, C.R., Ray, J., Schuch, W. and Grierson, D. (1990) Expression of a truncated tomato polygalacturonase gene inhibits expression of the endogenous gene in transgenic plants. Mol. Gen. Genet 224: 477-481.
52. Smith, H.A., Swaney, S.L., Parks, T.D., Wernsman, E.A. and Dougherty, W.G. (1994) Transgenic plant virus resistance mediated by untranslatable sense RNAs: expression, regulation and fate of non-essential RNAs. Plant Cell, 6, 1441-1453.
53. Stam, M., de Bruln, R., Kenter, S., van der Hoorn, R.A.L., van Blockland, R., Mol, J.N.M. and Kooter, J.M. (1997) Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats. Plant J. 12, 63-82.
54. Van Biockland, R., van der Geest, N., Mol, J.N.M. and Kooter, J.M. (1994) Transgene-mediated suppression of chalcone synthase expression in Petunia hybrida results from an increase in RNA turnover. Plant J. 6, 861-877.
55. Wessler, S.R. (1996) Plant retrotransposons - turned on by stress. Current Biol. 6, 959-961.