High-level extracellular production of penicillin acylase by genetic engineering of Escherichia coli

Journal of Chemical Technology and Biotechnology

Volumn 76, Issue 10, 2001, Pages 1030-1037

  Lin, Wen Jer ^a   Huang, Shin Wei ^a   Perry Chou, C ^a  

^a FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Bacteriocin release protein; Escherichia coli; Inclusion body; Penicillin acylase; Post translational processing; Protein release

Indexed keywords

ESCHERICHIA COLI; GENES; GENETIC ENGINEERING; OPTIMIZATION; PHYSIOLOGY;

BODY FORMATION;

ENZYMES;

BACTERIOCIN; MITOMYCIN C; PENICILLIN AMIDASE;

BIOTECHNOLOGY;

ARTICLE; BACTERIUM CULTURE; BIOREACTOR; CELL FUNCTION; CELL INCLUSION; CONCENTRATION (PARAMETERS); ENZYME ASSAY; ENZYME RELEASE; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXTRACELLULAR SPACE; GENE EXPRESSION; GENETIC ENGINEERING; HOST; MICROBIOLOGICAL PARAMETERS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING;

EID: 0034794414     PISSN: 02682575     EISSN: None     Source Type: Journal    
DOI: 10.1002/jctb.475     Document Type: Article

References (33)

1. Strategies for achieving high-level expression of genes in Escherichia coli
2. Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli
3. Alternative regulation principles for the production of recombinant proteins in Escherichia coli
4. Formation of recombinant protein inclusion bodies in Escherichia coli
5. Factors influencing inclusion body formation in the production of a fused protein Escherichia coli
6. A method for increasing the yield of properly folded recombinant fusion proteins: Single-chain immunotoxins from renaturation of bacterial inclusion bodies
7. Optimization of the solubilization and renaturation of fish growth hormone produced by Escherichia coli
8. Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E coli
9. Structure and morphology of protein inclusion bodies in Escherichia coli
10. Engineering Escherichia coli to secrete heterologous gene products
11. Optimization of bacteriocin-release-protein-induced protein release by Escherichia coli: Extracellular production of the periplasmic molecular chaperone FaeE
12. Bacteriocin release protein: Mode of action, structure, and biotechnological application
13. Penicillin acylase: Enzyme production and its application in the manufacture of 6-APA
14. Penicillin acylases: Applications and potentials
15. Primary structure requirements for the maturation in vivo of penicillin acylase from Escherichia coli ATCC 11105
16. Genetic manipulation to identify limiting steps and develop strategies for high-level expression of penicillin acylase in Escherichia coli
17. Molecular basis of the exclusive low-temperature synthesis of an enzyme in E coli: Penicillin acylase
18. Carbon regulation and the role in nature of the Escherichia coli penicillin acylase (pac) gene
19. Periplasmic aggregation limits the proteolytic maturation of the Escherichia coli Penicillin G amidase precursor polypeptide
20. Localization and characterization of inclusion bodies in recombinant Escherichia coli cells overproducing penicillin G acylase
21. Novel strategy for efficient screening and construction of host/vector systems to overproduce penicillin acylase in Escherichia coli
22. Cloning, expression and release of native and mutant cloacin DF13 across the cytoplasmic membrane of Escherichia coli
23. Preparation and storage of competent Escherichia coli cells
24. Regulation of penicillin acylase in Escherichia coli by cyclic AMP
25. The isolation and kinetics of penicillin amidase from Escherichia coli
26. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
27. Effect of SecB chaperone on production of periplasmic penicillin acylase in Escherichia coli
28. Escherichia coli Skp chaperone coexpression improves solubility and phage display of single-chain antibody fragments
29. Intramolecular autoproteolysis initiates the maturation of penicillin amidase from Escherichia coli
30. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature
31. DegP coexpression minimizes inclusion body formation upon overproduction of recombinant penicillin acylase in Escherichia coli