Deletion mutagenesis of large (12-kb) plasmids by a one-step pcr protocol

BioTechniques

Volumn 31, Issue 4, 2001, Pages 722-724

  Wang, J ^a   Wilkinson, M F ^a  

^a UNIVERSITY OF TEXAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA TEMPLATE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE DELETION; GENE MAPPING; MUTAGENESIS; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

EID: 0034793901     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314bm01     Document Type: Article

References (9)

1. A splicing-dependent regulatory mechanism that detects translation signals
2. Effective amplification of long targets from cloned inserts and human genomic DNA
3. General concepts for PCR primer design
4. A simple and rapid method for generating a deletion by PCR
5. Inverse PCR-generated internally deleted constructs for direct characterization of promoter regulatory regions
6. Site-directed mutagenesis: A two-step method using PCR and DpnI
7. Site-specific mutagenesis of almost any plasmid using a PCR-based version of unique site elimination
8. Site-directed mutagenesis of large (13-kb) plasmids in a single-PCR procedure
9. Adaptation of inverse PCR to generate an internal deletion