PNA-based light-up probes for real-time detection of sequence-specific PCR products

BioTechniques

Volumn 31, Issue 4, 2001, Pages 766-771

  Wolffs, P ^a   Knutsson, R ^a   Sjoback R ^a   Radstrom P ^a  

^a LUND INSTITUTE OF TECHNOLOGY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; DNA; MONITORING; OPTIMIZATION; ORGANIC ACIDS;

FLUORESCENT SIGNALS;

BIOTECHNOLOGY;

PEPTIDE NUCLEIC ACID; THIAZOLE ORANGE;

ARTICLE; DNA HYBRIDIZATION; DNA PROBE; DNA SEQUENCE; FLUORESCENCE; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; YERSINIA ENTEROCOLITICA;

EID: 0034793662     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314st01     Document Type: Article

References (20)

1. Quantitation of hepatitis B virus genomic DNA by real-time detection PCR
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3. Enhanced PCR amplification of VNTR locus D1S80 using peptide nucleic acid (PNA)
4. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen-bonding rules
5. Oligonucleotide drugs. A change of backbone
6. Rapid and specific detection of PCR products using light-up probes
7. Detection of the hereditary hemochromatosis gene mutation by real-time fluorescence polymerase chain reaction and peptide nucleic acid clamping
8. Detection of pathogenic Yersinia enterocolitica in enrichment media and pork by a multiplex PCR: A study of sample preparation and PCR-inhibitory components
9. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization
10. Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide
11. PNA molecular beacons for rapid detection of PCR amplicons
12. Single base pair mutation analysis by PNA directed PCR clamping
13. Sequence-specific purification of nucleic acids by PNA-controlled hybrid selection
14. Quantitative detection of Borrelia burgdorferi by realtime PCR
15. Peptide nucleic acid pre-gel hybridization: An alternative to Southern hybridization
16. Light-up probes; thiazole orange conjugated PNA for detection of target nucleic acid in homogeneous solution
17. Peptide nucleic acidmediated PCR clamping as a useful supplement in the determination of microbial diversity
18. Continuous fluorescence monitoring of rapid cycle DNA amplification
19. The LightCycler: A microvolume multisample fluorimeter with rapid temperature control
20. Identification of pathogenic Leptospira by TaqMan probe in a LightCycler