Exclusive amplification of cDNA template (EXACT) RT-PCR to avoid amplifying contaminating genomic pseudogenes

BioTechniques

Volumn 31, Issue 4, 2001, Pages 776-782

  Smith, R D ^a   Ogden, C W ^a   Penny, M A ^a  

^a NORTHWICK PARK HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; GENES; RNA;

PSEUDOGENES;

BIOTECHNOLOGY;

COMPLEMENTARY DNA; DNA; RNA;

ARTICLE; GENE AMPLIFICATION; GENE TECHNOLOGY; GENOME; HUMAN; HUMAN CELL; MOLECULAR BIOLOGY; PSEUDOGENE; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0034788731     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314st03     Document Type: Article

References (11)

1. Appropriate controls for reverse transcription polymerase chain reaction (RT-PCR)
2. Human β-actin retropseudogenes interfere with RT-PCR
3. The DNA sequence of human chromosome 22
4. Type 1 HERV-K genome is spliced into subgenomic transcripts in the human breast tumor cell line T47D
5. Poly(A) cDNA-specific (PACS) RT-PCR: A quantative method for the measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA
6. Optimization of DNase I removal of contaminating DNA from RNA for use in quantitative RNA-PCR
7. Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of β-actin transcripts as quantitative reference
8. Vertebrate pseudogenes
9. Pitfalls of processed pseudogenes in RT-PCR
10. Design and testing of β-actin primer for RT-PCR that do not co-amplify processed pseudogenes
11. Sensitive and specific cytokeratin 18 reverse transcription-polymerase chain reaction that excludes amplification of processed pseudogenes from contaminating genomic DNA