The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence

Molecular Microbiology

Volumn 41, Issue 6, 2001, Pages 1395-1408

  Holmes, Ann R ^a   McNab, Roderick ^b   Millsap, Kevin W ^c,f   Rohde, Manfred ^d   Hammerschmidt, Sven ^d   Mawdsley, Jane L ^e   Jenkinson, Howard F ^e  

^a UNIVERSITY OF OTAGO   (New Zealand)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

^d HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^e UNIVERSITY OF BRISTOL   (United Kingdom)

^f UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; AUTOLYSIN; BINDING PROTEIN; FIBRONECTIN; HEPARIN; MEMBRANE PROTEIN; PNEUMOLYSIN; POLYPEPTIDE; PROTEIN; PROTEIN FBP54; PROTEIN PAVA; PROTEIN PSAA; PROTEIN PSPA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BACTERIAL GROWTH; BACTERIAL MEMBRANE; BACTERIAL VIRULENCE; BACTERIUM ADHERENCE; BACTERIUM ISOLATE; BACTERIUM MUTANT; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; CELL STRUCTURE; CELL SURFACE; CONTROLLED STUDY; ENTEROCOCCUS FAECALIS; GENETIC CODE; IMMUNOELECTRON MICROSCOPY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN IMMOBILIZATION; PROTEIN LOCALIZATION; PROTEIN SYNTHESIS; SEPSIS; SIGNAL TRANSDUCTION; STREPTOCOCCUS INFECTION; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES;

ADHESINS, BACTERIAL; ANIMALS; BACTERIAL ADHESION; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; BASE SEQUENCE; CARRIER PROTEINS; DNA PRIMERS; DNA, BACTERIAL; FIBRONECTINS; GENE EXPRESSION; GENES, BACTERIAL; HUMANS; MICE; MICE, INBRED BALB C; MICROSCOPY, IMMUNOELECTRON; PNEUMOCOCCAL INFECTIONS; SEPSIS; STREPTOCOCCUS PNEUMONIAE; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; POSIBACTERIA; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES;

EID: 0034786512     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02610.x     Document Type: Article

References (75)

1. Streptococcus pneumoniae: Virulence factors, pathogenesis, and vaccines
2. Some aspects of the pneumococcal carrier state
3. Virulence factors among gram-positive bacteria in experimental endocarditis
4. A pneumolysin-negative mutant of Streptococcus pneumoniae causes chronic bacteremia rather than acute sepsis in mice
5. Differences in virulence for mice among Streptococcus pneumoniae strains of capsular types 2, 3, 4, 5, and 6 are not attributable to differences in pneumolysin production
6. α-enolase of Streptococcus pneumoniae is a plasmin(ogen)-binding protein displayed on the bacterial cell surface
7. Additive attenuation of virulence of Streptococcus pneumoniae by mutation of the genes encoding pneumolysin and other putative pneumococcal virulence proteins
8. Contribution of autolysin to virulence of Streptococcus pneumoniae
9. Pneumolysin, a protein toxin from Streptococcus pneumoniae, induces nitric oxide production from macrophages
10. The pspC gene of Streptococcus pneumoniae encodes a polymorphic protein PspC, which elicits cross-reactive antibodies to PspA and provides immunity to pneumococcal bacteremia
11. Diacylglycerol kinase is involved in regulation of expression of the lantibiotic mutacin II of Streptococcus mutans
12. The com locus controls genetic transformation in Streptococcus pneumoniae
13. Genomic sequencing
14. Construction and evaluation of new drug-resistance cassettes for gene disruption mutagenesis in Streptococcus pneumoniae, using an ami test platform
15. Cloning, sequencing, and expression of a fibronectin/ fibrinogen-binding protein from group A streptococci
16. Differential effects of the streptococcal fibronectin-binding protein FBP54, on adhesion of group A streptococci to human buccal cells and HEp-2 tissue culture cells
17. Serum opacity factor is a major fibronectin-binding protein and a virulence determinant of M type 2 Streptococcus pyogenes
18. Pneumococcal surface protein A (PspA) is serologically highly variable and is expressed by all clinically important capsular serotypes of Streptococcus pneumoniae
19. The pathogenesis of pneumococcal disease. A molecular model
20. PspC and a pneumococcal surface protein, binds human factor H
21. Role of the major pneumococcal autolysin in the atypical response of a clinical isolate of Streptococcus pneumoniae
22. Fibronectin binding protein and host cell tyrosine kinase are required for internalization of Staphylococcus aureus by epithelial cells
23. LytB, a novel pneumococcal murein hydrolase essential for cell separation
24. Protective immune response against Streptococcus pyogenesin mice after intranasal vaccination with the fibronectin-binding protein SfbI
25. Characterization of binding of human lactoferrin to pneumococcal surface protein A
26. SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component
27. Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae
28. Multiple adhesins of streptococci
29. An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae
30. Electrophoresis and other electro-kinetic phenomena
31. Candida albicans binding to the oral bacterium Streptococcus gordonii involves multiple adhesin-receptor interactions
32. Signal peptides: Exquisitely designed transport promoters
33. Protein F2, a novel fibronectin-binding protein from Streptococcus pyogenes, possesses twodomains
34. Charge properties of microbial cell surfaces
35. Insertional inactivation of the gene encoding a 76-kilodalton cell surface polypeptide in Streptococcus gordonii Challis has a pleiotropic effect on cell surface composition and properties
36. Streptococcal adhesion and colonization
37. Inactivation of the gene encoding surface protein SspA in Streptococcus gordonii DL1 affects cell interactions with human salivary agglutinin and oral actinomyces
38. Pneumococcal virulence factors: Structure and function
39. Fibronectin receptors from gram-positive bacteria: Comparison of active sites
40. Systemic and mucosal immunizations with fibronectin-binding protein FBP54 induce protective immune responses against Streptococcus pyogenes challenge in mice
41. A functional genomic analysis of type 3 Streptococcus pneumoniae virulence
42. Cell surface polypeptide CshA mediates binding of Streptococcus gordonii to other oral bacteria and to immobilized fibronectin
43. Cell wall-anchored CshA polypeptide (259 kilodaltons) in Streptococcus gordonii forms surface fibrils that confer hydrophobic and adhesive properties
44. Requirement for capsule in colonization by Streptococcus pneumoniae
45. Fibronectin-binding protein I of Streptococcus pyogenes is a promising adjuvant for antigens delivered by mucosal route
46. The fibronectin-binding protein of Streptococcus pyogenes. SfbI, is involved in the internalization of group A streptococci by epithelial cells
47. Two distinct pathways for the invasion of Streptococcus pyogenes in non-phagocytic cells
48. Proteolytic cleavage and cell wall anchoring at the LPXTG motif of surface proteins in gram-positive bacteria
49. Amplification of flanking sequences by inverse PCR
50. A matrix form of fibronectin mediates enhanced binding of Streptococcus pyogenes to host tissue
51. Streptococcus pyogenes F protein promotes invasion of HeLa cells
52. A two-domain mechanism for group A streptococcal adherence through protein F to the extracellular matrix
53. Role of integrins and fibronectin in the entry of Streptococcus pyogenes into cells via protein F1
54. A major surface protein on group A streptococci is a glyceraldehyde-3-phosphate-dehydrogenase with multiple binding activity
55. Regulation of the phosphorylation of human pharyngeal cell proteins by group A streptococcal surface dehydrogenase: Signal transduction between streptococci and pharyngeal cells
56. α-enolase, a novel strong plasmin(ogen) binding protein on the surface of pathogenic streptococci
57. The contribution of pneumolysin to the pathogenicity of Streptococcus pneumoniae
58. Novel pneumococcal surface proteins: Role in virulence and vaccinepotential
59. Genetic identification of exported proteins in Streptococcus pneumoniae
60. Identification and characterization of a novel fibronectin-binding protein on the surface of group A streptococci
61. Biological role of the pneumococcal amidase. Cloning of the lytA gene in Streptococcus pneumoniae
62. Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae
63. An extended -10 promoter alone directs transcription of the DpnII operon of Streptococcus pneumoniae
64. Identification of D motif epitopes in Staphylococcus aureus fibronectin-binding protein for the production of antibody inhibitors of fibronectin binding
65. Novel streptococcal-integration shuttle vectors for gene cloning and inactivation
66. Pneumococcal surface protein A inhibits complement activation by Streptococcus pneumoniae
67. Pathogenesis of pneumococcal infection
68. Adherence of Streptococcus pneumoniae to immobilized fibronectin
69. Phagocytosis as a surface phenomenon. I. Contact angles and phago-cytosis of non-opsonised bacteria
70. The -16 region of Bacillus subtilis and other gram-positive bacterial promoters
71. Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector
72. Novel surface attachment mechanism of the Streptococcus pneumoniae protein PspA
73. Truncated forms of PspA that are secreted from Streptococcus pneumoniae and their use in functional studies and cloning of the pspA gene
74. Recombinant PhpA protein and a unique histidine motif-containing protein from Streptococcus pneumoniae protects mice against intranasal pneumococcal challenge