Inactivation of the 7B2 inhibitory CT peptide depends on a functional furin cleavage site

Journal of Neurochemistry

Volumn 79, Issue 2, 2001, Pages 437-444

  Hwang, J R ^a   Lindberg, I ^a  

^a LOUISIANA STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

7B2; Furin; PC2; Prohormone convertase; Secretory pathway; Trans Golgi network

Indexed keywords

FURIN; HISTIDINE; LYSINE; PEPTIDE; PROTEIN; RECOMBINANT PROTEIN; SUBTILISIN;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MUTATION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ANIMALS; CARBOXYPEPTIDASE H; CARBOXYPEPTIDASES; CELL LINE; CELL MEMBRANE; ENZYME ACTIVATION; FURIN; GOLGI APPARATUS; HUMANS; MUTATION; NERVE TISSUE PROTEINS; NEUROENDOCRINE SECRETORY PROTEIN 7B2; PEPTIDE FRAGMENTS; PITUITARY HORMONES; PROPROTEIN CONVERTASE 2; RATS; SUBTILISINS; TIME FACTORS;

ANIMALIA; EUKARYOTA;

EID: 0034775434     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.2001.00594.x     Document Type: Article

References (21)

1. Structure - Function analysis of the 7B2 CT peptide
2. Mutations in the catalytic domain of prohormone convertase 2 result in decreased binding to 7B2 and loss of inhibition with 7B2 C-terminal peptide
3. The neuroendocrine polypeptide 7B2 is a precursor protein
4. 7B2 is a specific intracellular binding protein of the prohormone convertase PC2
5. Residues unique to the prohormone convertase PC2 modulate its autoactivation, binding to 7B2, and enzymatic activity
6. Mapping of the domain in the neuroendocrine protein 7B2 important for its helper function towards prohormone convertase PC2
7. Identification of the region within the neuroendocrine polypeptide 7B2 responsible for the inhibition of prohormone convertase PC2
8. Enzymatic characterization of immunopurified prohormone convertase 2. Potent inhibition by a 7B2 peptide fragment
9. The neuroendocrine polypeptide 7B2 is an endogenous inhibitor of prohormone convertase PC2
10. The cell biology of the prohormone convertases PC1 and PC2
11. Mechanism of the facilitation of PC2 maturation by 7B2: Involvement in ProPC2 transport and activation but not folding
12. A 36 residue peptide contains all of the information required for 7B2-mediated activation of proPC2 to PC2
13. Processing and sorting of the prohormone convertase 2 propeptide
14. The neuroendocrine precursor 7B2 is a sulfated protein proteolytically processed by a ubiquitous furin-like convertase
15. Neuroendocrine protein 7B2 is essential for proteolytic conversion and activation of proprotein convertase 2 in vivo
16. Construction of mutant and chimeric genes using the polymerase chain reaction
17. 7B2 Facilitates the maturation of proPC2 in neuroendocrine cells and is required for the expression of enzymatic activity
18. Involvement of a polyproline helix-like structure in the interaction of 7B2 with prohormone convertase 2
19. Internal cleavage of the inhibitory 7B2 carboxyl-terminal peptide by PC2. A potential mechanism for its inactivation
20. Structural elements of PC2 required for interaction with its helper protein 7B2
21. Proteolytic processing in the secretory pathway