Autofluorescence characteristics of immortalized and carcinogen-transformed human bronchial epithelial cells

Journal of Biomedical Optics

Volumn 6, Issue 1, 2001, Pages 31-40

  Pitts, Jonathan D ^a   Sloboda, Roger D ^a   Dragnev, Konstantin H ^b   Dmitrovsky, Ethan ^b   Mycek, Mary Ann ^a,c  

^a DARTMOUTH COLLEGE   (United States)

^b DARTMOUTH MEDICAL SCHOOL   (United States)

^c NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL ORGANS; CELLS; CYTOLOGY; MICROSCOPIC EXAMINATION; NONINVASIVE MEDICAL PROCEDURES; ONCOLOGY; SPECTROSCOPY; TISSUE;

BRONCHIAL EPITHELIAL CELL; CELLULAR AUTOFLUORESCENCE; CONFOCAL MICROSCOPY; FLUORESCENCE SPECTROSCOPY; LUNG CANCER DETECTION; TOBACCO-CARCINOGEN TRANSFORMATION;

FLUORESCENCE;

CARCINOGEN;

ARTICLE; BRONCHUS; CELL LINE; CHEMISTRY; CONFOCAL MICROSCOPY; CYTOLOGY; DRUG EFFECT; EPITHELIUM CELL; FLUORESCENCE MICROSCOPY; FLUOROMETRY; HUMAN; PATHOLOGY; PHYSIOLOGY; PLANT; SPECTROFLUOROMETRY; TOBACCO;

BRONCHI; CARCINOGENS; CELL LINE, TRANSFORMED; EPITHELIAL CELLS; FLUOROMETRY; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; PLANTS, TOXIC; SPECTROMETRY, FLUORESCENCE; TOBACCO;

EID: 0034745201     PISSN: 10833668     EISSN: None     Source Type: Journal    
DOI: 10.1117/1.1333057     Document Type: Article

References (82)

1. R. Richards-Kortum and E. Sevick-Muraca, "Quantitative optical spectroscopy for tissue diagnosis," Annu. Rev. Phys. Chem. 47, 555-606 (1996).
2. R. S. DaCosta, B. C. Wilson, and N. E. Marcon, "Light-induced fluorescence endoscopy of the gastrointestinal tract," Gastrointest Endosc 10(1), 37-69 (2000).
3. G. Wagnières, W. Star, and B. Wilson, "In vivo fluorescence spectroscopy and imaging for oncological applications," Photochem. Photobiol. 68(5), 603-632 (1998).
4. I. Bigio and J. Mourant, "Ultraviolet and visible spectroscopies for tissue diagnostics: Fluorescence spectroscopy and elastic-scattering spectroscopy," Phys. Med. Bio. 42, 803-814 (1997).
5. W. S. Classman, C. H. Liu, G. C. Tang, S. Lubics, and R. R. Alfano, "Ultraviolet excited fluorescence spectra from nonmalignant and malignant tissues of the gynecological tract," Lasers Life Sci. 5, 49-58 (1994).
6. N. Ramanujam, A. Mahadevan, M. Follen-Mitchell, S. Thomsen, E. Silva, and R. Richards-Kortum, "Fluorescence spectroscopy of the cervix," Clin. Consult. Obstetrics Gynecol. 6(1), 62-69 (1994).
7. N. Ramanujam, M. Follen-Mitchell, A. Mahadevan, S. Thomsen, A. Malpica, T. Wright, N. Atkinson, and R. Richards-Kortum, "Spec-troscopic diagnosis of cervical intraepithelial neoplasia (CIN) in vivo using laser-induced fluorescence spectra at multiple excitation wave-lengths," Lasers Surg. Med. 19, 63-74 (1996).
8. N. Ramanujam, M. Follen-Mitchell, A. Mahadevan, S. Thomsen, A. Malpica, T. Wright, N. Atkinson, and R. Richards-Kortum, "Development of a multivariate statistical algorithm to analyze human cervical tissue fluorescence spectra acquired in vivo" Lasers Surg. Med. 19, 46-62 (1996).
9. N. Ramanujam, M. F. Mitchell, A. Mahadevan, S. Warren, S. Thomsen, E. Silva, and R. Richards-Kortum, "In vivo diagnosis of cervical intraepithelial neoplasia using 337-nm-excited laser-induced fluorescence," Proc. Natl. Acad. Sci. U.S.A. 91, 10193-10197 (1994).
10. M. A. D'Hallewin, L. Baert, and H. Vanherzeele, "Fluorescence imaging of bladder cancer," Acta Urol. Belg. 62(4), 49-52 (1994).
11. F. Konig, F. J. McGovern, A. F. Althausen, T. F. Deutsch, and K. T. Schomacker, "Laser-induced autofluorescence diagnosis of bladder cancer," J. Urol. (Baltimore) 156(5), 1597-1601 (1996).
12. M. Anidjar, O. Cussenot, S. Avillier, D. Ettori, P. Teillac, and A. LeDuc, "The role of laser-induced autofluorescence spectroscopy in bladder tumor detection: Dependence on the excitation wavelength," in Advances in Optical Biopsy and Optical Mammography, R. R. Alfano, Ed., pp. 130-142, The New York Academy of Sciences, New York (1998).
13. S. Lam, J. Hung, and B. Palcic, "Mechanism of detection of early lung cancer by ratio fluorometry," Lasers Life Sci. 4(2), 67-73 (1991).
14. S. Lam, C. MacAulay, J. Hung, J. LeRiche, A. E. Profio, and B. Palcic, "Detection of dysplasia and carcinoma in situ with a lung imaging fluorescence endoscope device," J. Thorac. Cardiovasc. Surg. 105(6), 1035-1040 (1993).
15. S. Lam, C. J. Macaulay, C. Leriche, N. Ikeda, and B. Palcic, "Early localization of bronchogenic carcinoma," Diagnostic Therapeutic Endosc. 1, 75-78 (1994).
16. S. Lam, T. Kennedy, M. Unger, Y. E. Miller, D. Gelmot, V. Rusch, B. Gipe, D. Howard, J. C. LeRiche, A. Coldman, and A. F. Gazdar, "Localization of bronchial intraepithelial neoplastic lesions by fluorescence bronchoscopy," Chest 113(3), 696-702 (1998).
17. R. R. Alfano, G. Tang, A. Pradhan, M. Bleich, D. S. J. Choy, and E. Opher, "Steady-state and time-resolved laser fluorescence from normal and tumor lung and breast tissues," J. Tumor Marker Oncol. 3, 165-73 (1988).
18. G. C. Tang, A. Pradhan, and R. Alfano, "Spectroscopic differences between human cancer and normal lung and breast tissues," Lasers Surg. Med. 9, 290-295 (1989).
19. R. Cubeddu, A. Pifferi, P. Taroni, A. Torricelli, and G. Valentini, "Noninvasive absorption and scattering spectroscopy of bulk diffuse media: An application to the optical characteristics of human breast," Appl. Phys. Lett. 74(6), 874-876 (1999).
20. P. Gupta, S. Kumar Majumder, and A. Uppal, "Breast cancer diagnosis using N2 laser-excited autofluorescence spectroscopy," Lasers Surg. Med. 21, 417-422 (1997).
21. G. C. Tang, A. Pradhan, W. Sha, J. Chen, C. H. Liu, S. J. Wahl, and R. R. Alfano, "Pulsed and cw laser fluorescence spectra from cancerous, normal, and chemically treated normal human breast and lung tissues," Appl. Opt. 28(12), 2337-2342 (1989).
22. T. Vo-Dinh, M. Panjehpour, and B. Overholt, "Laser-induced fluorescence for esophageal cancer and dysplasia diagnosis," in Advances in Optical Biopsy and Optical Mammography, R. R. Alfano, Ed., pp. 116-122, The New York Academy of Sciences, New York (1998).
23. T. Vo-Dinh, M. Panjehpour, B. Overholt, C. Farris, F. Buckley, and R. Sneed, "In vivo cancer diagnosis of the esophagus using differential normalized fluorescence (DNF) indices," Lasers Surg. Med. 16, 41-47 (1995).
24. M. Panjehpour, B. Overholt, T. Vo-Dinh, R. Haggutt, D. Edwards, and F. P. Buckley III, "Endoscopic fluorescence detection of high-grade dysplasia in Barrett's esophagus," Gastroenterology 111, 93-101 (1996).
25. M. Keijzer, R. Richards-Kortum, S. Jacques, and M. Feld, "Fluorescence spectroscopy of turbid media: Autofluorescence of the human aorta," Appl. Opt. 28(20), 4286-4292 (1989).
26. B. Chwirot, S. Chwirot, W. Jedrzejczyk, M. Jackowski, A. Raczynska, J. Winczakiewicz, and J. Dobber, "Ultraviolet laser-induced fluorescence of human stomach tissues: Detection of cancer tissues by imaging techniques," Lasers Surg. Med. 21, 149-158 (1997).
27. S. K. Majumder, A. Uppal, and P. K. Gupta, "In vitro diagnosis of human uterine malignancy using N-2 laser-induced autofluorescence spectroscopy," Curr. Sci. 70(9), 833-836 (1996).
28. Y. P. Sinichkin, S. R. Utz, A. H. Mavliutov, and H. A. Pilipenko, "In vivo fluorescence spectroscopy of the human skin: Experiments and models," J. Biomed. Opt. 3(2), 201-211 (1998).
29. Y. P. Sinichkin, S. R. Utts, I. V. Meglinskii, and E. A. Pilipenko, "Spectroscopy of human skin in vivo: II Fluorescence spectra," Mol. Spectrosc. 80(3), 383-389 (1996).
30. S. R. Utts, Y. P. Sinichkin, and E. A. Pilipenko, "Laser fluorescence spectroscopy of human skin in vivo: The effect of erythema," Opt. Spectrosc. 76(5), 771-774 (1994).
31. E. W. J. van der Breggen, A. I. Rem, M. M. Christian, C. J. Yang, K. H. Calhoun, H. J. C. M. Sterenborg, and M. Motamedi, "Spectroscopic detection of oral and skin tissue transformation in a model for squamous cell carcinoma: Autofluorescence versus systematic aminolevulinic acid-induced fluorescence," IEEE J. Sel. Top. Quantum Electron. 2(4), 997-1007 (1996).
32. K. Konig, A. Ruck, and H. Schneckenburger, "Fluorescence detection and photodynamic activity of endogenous protoporphyrin in human skin," Opt. Eng. 31(7), 1470-1474 (1992).
33. C. Kapadia, F. Cutruzzola, K. O'Brien, M. Stetz, R. Enriquez, and L. Deckelbaum, "Laser-induced fluorescence spectroscopy of human colonic mucosa," Gastroenterology 99, 150-157 (1990).
34. R. Cothren, R. Richards-Kortum, M. Sivak, M. Fitzmaurice, R. Rava, G. Boyce, M. Doxtader, R. Blackman, T. Ivanc, G. Hayes, M. Feld, and R. Petras, "Gastrointestinal tissue diagnosis by laser-induced fluorescence spectroscopy at endoscopy," Gastrointest. Endosc. 36, 105-111 (1990).
35. K. T. Schomacker, J. K. Frisoli, C. C. Compton, T. J. Flotte, J. M. Richter, and T. F. Deutsch, "Ultraviolet laser-induced fluorescence of colonic polyps," Gastroenterology 102, 1155-1160 (1992).
36. K. T. Schomacker, J. K. Frisoli, C. C. Compton, T. J. Flotte, J. M. Richter, N. S. Nishioka, and T. F. Deutsch, "Ultraviolet laser-induced fluorescence of colonic tissue: Basic biology and diagnostic potential," Lasers Surg. Med. 12, 63-78 (1992).
37. R. Marchesini, M. Brambilla, and E. Pignoli, "Light-induced fluorescence spectroscopy of adenomas, adenocarcinomas, and non-neoplastic mucosa in human colon 1. In vitro measurements," J. Photochem. Photobiol., B 14, 219-230 (1992).
38. G. Bottiroli, A. C. Croce, D. Locatelli, R. Marchesini, E. Pignoli, S. Tomatis, C. Cuzzoni, S. DiPalma, M. Dalfante, and P. Spinelli, "Natural fluorescence of normal and neoplastic human colon: A comprehensive "ex vivo" study," Lasers Surg. Med. 16, 48-60 (1995).
39. Y. Yang, G. C. Tang, M. Bessler, and R. R. Alfano, "Fluorescence spectroscopy as a photonic pathology method for detecting colon cancer," Lasers Life Sci. 6(4), 259-276 (1995).
40. R. Cothren, M. Sivak, J. Van Dam, R. Petras, M. Fitzmaurice, and J. Crawford, "Detection of dysplasia at colonoscopy using laser-induced fluorescence: A blinded study," Gastrointest. Endosc. 44, 168-76 (1996).
41. M.-A. Mycek, K. Vishwanath, K. T. Schomacker, and N. S. Nishioka, "Fluorescence spectroscopy for in vivo discrimination of premalignant colonic lesions," in Biomedical Topical Meetings, OSA Technical Digest, pp. 11-13, Optical Society of America, Washington, DC (2000).
42. J. E. Aubin, "Autofluorescence of viable cultured mammalian cells," J. Histochem. Cytochem. 27(1), 36-43 (1979).
43. K. Konig, H. Schneckenburger, A. Ruck, R. Steiner, and H. Walt, "Laser-induced autofluorescence of cells and tissue," Proc. SPIE 1887, 213-221 (1993).
44. A. C. Croce, A. Spano, D. Locatelli, S. Barni, and G. Bottiroli, "Dependence of fibroblast autofluorescence properties on normal and transformed conditions. Role of metabolic activity," Photochem. Photobiol. 69(3), 364-374 (1999).
45. M. Anidjar, O. Cussenot, J. Blais, O. Bourdon, S. Avrillier, D. Ettori, J.-M. Villette, J. Fiet, P. Teilllac, and A. LeDuc, "Argon laser-induced autofluorescence may distinguish between normal and tumor human urothelial cells: A microspectrofluorimetric study," J. Urol. (Baltimore) 155(5), 1771-1774 (1996).
46. P. Gedigk and E. Bontke, "Detection of hydrolytic enzymes in lipopigments," Z. Zellforsch Mikrosk Anat. 44, 495-518 (1956).
47. E. Essner and A. B. Novikoff, "Human hepatocellular pigments and lysosomes," J. Ultrastruct. Res. 3, 374-391 (1960).
48. A. N. Siakotos and K. D. Munkres, "Recent developments in the isolation and properties of autofluorescent lipopigments," in Ceroidlipofuscinosis, N. K. D. Armstrong and J. A. Rider, Eds., pp. 167-178, Elsevier, New York (1982).
49. G. E. Eldred, G. V. Miller, W. S. Stark, and L. Feeney-Burns, "Lipofuscin: Resolution of discrepant fluorescence data," Science 216, 757-759 (1982).
50. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Second Edition), 2nd ed., Kluwer Academic/Plenum, New York (1999).
51. W. Balcavage, "On the fluorescence lifetime of tryptophan and proteins," Photochemistry 7(3), 309-323 (1976).
52. R. F. Chen, "Fluorescence quantum yields of tryptophane and tyrosine," Anal. Lett. 1(1), 35-42 (1967).
53. A. Szabo and D. Rayner, "Fluorescence decay of tryptophan conformers in aqueous solution," J. Am. Chem. Soc. 102(2), 554-563 (1980).
54. J. C. Zhang, H. E. Savage, P. G. Sacks, T. Delohery, R. R. Alfano, A. Katz, and S. P. Schantz, "Innate cellular fluorescence reflects alterations in cellular proliferation," Lasers Surg. Med. 20, 319-331 (1997).
55. S. Ganesan, P. G. Sacks, Y. Yang, A. Katz, M. Al-Rawi, H. E. Savage, S. P. Schantz, and R. R. Alfano, "Native fluorescence spectroscopy of normal and malignant epithelial cells," Cancer Biochem. Biophys. 16, 365-373 (1998).
56. D. Parmeswarn, S. Ganesan, R. Nalini, P. Aruna, V. Veeraganesh, and R. R. Alfano, "Native cellular fluorescence characteristics of normal and malignant epithelial cells from human larynx," Proc. SPIE 2979, 759-764 (1997).
57. C. Brookner, M. Follen, I. Boiko, J. Galvan, S. Thomsen, A. Malpica, S. Suzuki, R. Lotan, and R. Richards-Kortum, "Autofluorescence patterns in short-term cultures of normal cervical tissue," Photochem. Photobiol. 71(6), 730-736 (2000).
58. D. L. Heintzelman, R. Lotan, and R. Richards-Kortum, "Characterization of the autofluorescence of polymorphonuclear leukocytes, mononuclear leukocytes, and cervical epithelial cancer cells for improved spectroscopic discrimination of inflammation from dysplasia," Photochem. Photobiol. 71(3), 327-332 (2000).
59. R. Richards-Kortum, C. Brookner, I. Boiko, A. Malpica, S. Thomsen, R. Lotan, and M. Follen, "Biological basis of cervical tissue autofluorescence," in Biomedical Topical Meetings, OSA Technical Digest, pp. 209-211, Optical Society of America, Washington, DC (2000).
60. J. Qu, C. MacAulay, S. Lam, and B. Palcic, "Optical properties of normal and carcinomatous bronchial tissue," Appl. Opt. 33(31), 7397-7405 (1994).
61. R. R. Alfano, G. C. Tang, A. Pradhan, W. Lam, D. S. J. Choy, and E. Opher, "Fluorescence spectra from cancerous and normal human breast and lung tissue," IEEE J. Quantum Electron. 23(10), 1806-1811 (1987).
62. J. Hung, S. Lam, J. LeRiche, and B. Palcic, "Autofluorescence of normal and malignant bronchial tissue," Lasers Surg. Med. 11, 99-105 (1991).
63. T. Glanzmann, J.-P. Ballini, H. van den Bergh, and G. Wagnieres, "Time-resolved spectrofluorometer for clinical tissue characterization during endoscopy," Rev. Sci. Instrum. 70(10), 4067-4077 (1999).
64. H. Yokomise, K. Yanagihara, T. Fukuse, T. Hirata, O. Ike, H. Mizuno, H. Wada, and S. Hitomi, "Clinical experience with lung-imaging fluorescence endoscope (LIFE) in patients with lung cancer," J. Bronchol. 4, 205-208 (1997).
65. D. R. Doiron, A. E. Profio, R. G. Vinvent, and T. J. Dougherty, "Fluorescence Bronchoscopy for detection of lung cancer," Chest 76, 27-32 (1979).
66. A. E. Profio, D. R. Doiron, O. J. Balchum, and G. C. Huth, "Fluorescence bronchoscopy for localization of carcinoma in situ," Med. Phys. 10(1), 35-39 (1983).
67. A. E. Profio, D. R. Doiron, and E. G. King, "Laser fluorescence bronchoscopy for localization of occult lung tumors," Med. Phys. 6(6), 523-525 (1979).
68. S. Lam, C. MacAulay, and B. Palcic, "Detection and localization of early lung cancer by imaging techniques," Chest 103, 12S-14S (1993).
69. S. Lam, J. Hung, S. M. Kennedy, J. C. LeRiche, S. Vedal, B. Nelems, C. E. MacAulay, and B. Palcic, "Detection of dysplasia and carcinoma in situ by ratio fluormetry," Am. Rev. Respir. Dis. 146, 1458-1461 (1992).
70. A. Pradhan, P. Pal, G. Durocher, L. Villeneuve, A. Balassy, F. Babai, L. Gaboury, and L. Blanchard, "Steady-state and time-resolved fluorescence properties of metastatic and nonmetastatic malignant cells from different species," J. Photochem. Photobiol., B 31, 101-112 (1995).
71. R. Reddel, Y. K. Ke, V. Gerwin, M. McMenamin, J. Lechner, R. Su, D. Brash, J.-B. Park, J. Limb Rhim, and C. Harris, "Transformation of human bronchial epithelial cells by infections by SV40 or adenovius-12 SV40 hybrid virus, or transfection via strontium phosphate coprecipitation with the plasmid containing SV40 early region genes," Cancer Res. 48, 1904-1909 (1988).
72. J. Langenfeld, F. Lonardo, H. Kiyokawa, T. Passalaris, M. Ahn, A. Rusch, and E. Dmitrovsky, "Inhibited transformation of immortalized human bronchial epithelial cells by retinoic acid is linked to cyclin E down-regulation," Oncogene 13, 1983-1999 (1996).
73. J. Langenfeld, H. Kiyokawa, D. Sekula, J. Boyle, and E. Dmitrovsky, "Post-translational regulation of cyclin D1 by retinoic acid: A chemoprevention mechanism," Proc. Natl. Acad. Sci. U.S.A. 94, 12070-12074 (1997).
74. J. Boyle, J. Langenfeld, F. Lonardo, P. Reczek, V. Rusch, M. Dawson, and E. Dmitrovsky, "Cyclin D1 proteolysis is a retinoid cancer chemoprevention signal in normal, immortalized, and transformed human bronchial epithelial cells," J. Natl. Cancer Inst. 91(4), 373-379 (1999).
75. F. Lonardo, V. Rusch, J. Langenfeld, E. Dmitrovsky, and D. S. Klimstra, "Overexpression of cyclins D1 and E is frequent in bronchial preneoplasia and precedes squamous cell carcinoma development," Cancer Res. 59, 2470-2476 (1999).
76. F. Jenkins and H. White, Fundamentals of Optics, McGraw-Hill, New York (1957).
77. I. D. Campbell and R. A. Dwenk, Biological Spectroscopy, Benjamin Cummings, Menlo Park, CA (1984).
78. P. G. Sacks, H. E. Savage, J. Levine, V. R. Kolli, R. R. Alfano, and S. P. Schantz, "Native cellular fluorescence identifies terminal squamous differentiation of normal oral epithelial cells in culture: A potential chemoprevention marker," Cancer Lett. (Shannon, Ireland) 104, 171-181 (1996).
79. M. A. Pollack, A. Taylor, J. Taylor, and R. J. Williams, "Vitamins in cancerous tissues I. Riboflavin," Cancer Res. 2, 739-745 (1942).
80. B. Chance and B. Schoener, "Fluorometric studies of flavin component of the respiratory chain," in Flavins and Flavoproteins, E. C. Slater, Ed., Elsevier, New York (1966).
81. B. Chance, P. Cohen, F. Jobis, and B. Schoener, "Intracellular oxidation-reduction rates in vivo. The microfluorometry of pyridine nucleotide gives a continuous measure of the oxidation state," Science 137, 499-508 (1962).
82. H. Andersson, T. Baechi, M. Hoechl, and C. Richter, "Autofluorescence of living cells," J. Microsc. 191, 1-7 (1998).