Reconstituted syntaxin 1A/SNAP25 interacts with negatively charged lipids as measured by lateral diffusion in planar supported bilayers

Biophysical Journal

Volumn 81, Issue 1, 2001, Pages 266-275

  Wagner, Michael L ^a   Tamm, Lukas K ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; LIPID; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLSERINE; SNARE PROTEIN; SYNAPTOBREVIN; SYNAPTOSOMAL ASSOCIATED PROTEIN 25; SYNTAXIN; TRIPHOSPHOINOSITIDE; 1-PALMITOYL-2-OLEOYLPHOSPHATIDYLCHOLINE; 2 OLEOYL 1 PALMITOYLPHOSPHATIDYLCHOLINE; ANION; CARRIER PROTEIN; LIPOSOME; MACROGOL DERIVATIVE; MEMBRANE ANTIGEN; MEMBRANE PROTEIN; NERVE PROTEIN; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE; POLYMER; SILICON DIOXIDE; SOLUBLE N ETHYLMALEIMIDE SENSITIVE FACTOR ATTACHMENT PROTEIN; SYNTAXIN 1; VESICULAR TRANSPORT PROTEIN;

ARTICLE; COMPLEX FORMATION; DIFFUSION; DIFFUSION COEFFICIENT; LIPID BILAYER; MEMBRANE FLUIDITY; MEMBRANE FUSION; MEMBRANE MODEL; PROTEIN LIPID INTERACTION; CHEMISTRY; CONFORMATION; FLUORESCENCE MICROSCOPY; MACROMOLECULE; METABOLISM; PROTEIN BINDING; SOLUBILITY;

ANIONS; ANTIGENS, SURFACE; CARRIER PROTEINS; DIFFUSION; LIPID BILAYERS; LIPOSOMES; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR CONFORMATION; NERVE TISSUE PROTEINS; PHOSPHATIDYLCHOLINES; PHOSPHATIDYLINOSITOL 4,5-DIPHOSPHATE; PHOSPHATIDYLSERINES; POLYETHYLENE GLYCOLS; POLYMERS; PROTEIN BINDING; QUARTZ; SNARE PROTEINS; SOLUBILITY; SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS; SYNAPTOSOMAL-ASSOCIATED PROTEIN 25; SYNTAXIN 1; VESICULAR TRANSPORT PROTEINS;

EID: 0034743458     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75697-4     Document Type: Article

References (44)

1. Assays of inorganic phosphate, total phosphate and phosphatases
2. Syntaxin: A synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones
3. Purification of an N-eythylmaleimide-sensitive protein catalyzing vesicular transport
4. Synaptotagmin: A calcium sensor on the synaptic vesicle surface
5. Functions of lipid rafts in biological membranes
6. 2+-dependent exocytosis
7. Poly(ethylene gycol) grafted to quartz: Analysis in terms of a site-dissociation model of electroosmotic fluid flow
8. SNARE membrane trafficking dynamics in vivo
9. SNAPs, a family of NSF attachment proteins involved in intracellular membrane fusion in animals and yeast
10. Formation and spreading of lipid bilayers on planar glass supports
11. The role of GTP-binding proteins in transport along the exocytic pathway
12. 2+-induced and GTP-analog-induced catecholamine release from permeabilized adrenal chromaffin cells
13. Neurotransmitter release: Four years of SNARE complexes
14. Synaptic vesicle membrane fusion complex: Action of clostridial neurotoxins on assembly
15. Disassembly of the reconstituted synaptic vesicle membrane fusion complex in vitro
16. Reconstitution of membrane fusion sites. A total internal reflection fluorescence microscopy study of influenza hemagglutinin-mediated membrane fusion
17. Membrane fusion and exocytosis
18. Binding of proteins to specific target sites in membranes measured by total internal reflection fluorescence spectroscopy
19. Formation of supported planar bilayers by fusion of vesicles to supported phospholipid monolayers
20. Phosphoinositides as spatial regulators of membrane traffic
21. Supported planar membranes in studies of cell-cell recognition in the immune system
22. The length of the flexible SNAREpin juxtamembrane region is a critical determinant of SNARE-dependent fusion
23. Three-dimensional structure of the neuronal-nSec1-syntaxin la complex
24. Structural basis of Rab effector specificity: Crystal structure of the small G protein Rab3A complexed with the effector domain of rabphilin-3A
25. Rapid and efficient fusion of phospholipid vesicles by the α-helical core of a SNARE complex in the absence on an N-terminal regulatory domain
26. The synaptic SNARE complex is a parallel four-stranded helical bundle
27. Protein sorting by transport vesicles
28. Supported membranes: Scientific and practical applications
29. Single-particle tracking: Applications to membrane dynamics
30. Lipid rafts and signal transduction
31. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of synaptic vesicle docking, activation, and fusion
32. SNAP receptors implicated in vesicle targeting and fusion
33. A synaptic vesicle membrane protein is conserved from mammals to Drosophila
34. 2+-independent SNARE complex formation
35. Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution
36. Lateral diffusion and fluorescence microscope studies on a monoclonal antibody specifically bound to supported phospholipid bilayers
37. Total internal reflectance fluorescence microscopy
38. Supported phospholipid bilayers
39. Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy
40. A vacuolar v-t SNARE complex, the predominant forms in vivo and on isolated vacuoles, is disassembled and activated for docking and fusion
41. Tethered polymer-supported planar lipid bilayers for reconstitution of integral membrane proteins: Silanepolyethyleneglycol-lipid as a cushion and covalent linker
42. SNAREpins: Minimal machinery for membrane fusion
43. N-ethylmaleimide-sensitive fusion protein: A trimeric ATPase whose hydrolysis of ATP is required for membrane fusion
44. Transport, capture and exocytosis of single synaptic vesicles at active zones