Sequential determination of ligands binding to discrete components in heterogeneous mixtures by iterative panning and blocking (IPAB)

Journal of Molecular Biology

Volumn 296, Issue 3, 2000, Pages 821-832

  Messmer, Bradley T ^a   Benham, Craig J ^b   Thaler, David S ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

C1q; Fibrinogen; Panning; Phage display; Selection strategy

Indexed keywords

AMINO ACID; COMPLEMENT COMPONENT C1; FIBRINOGEN; PEPTIDE LIBRARY;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; HUMAN; LIGAND BINDING; MOLECULAR INTERACTION; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN STRUCTURE;

EID: 0034711955     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3487     Document Type: Article

References (46)

1. Characterization of phage that bind plastic from phage-displayed random peptide libraries
2. Derivations of genotypes of some mutant deriviatives of Escherichia coli K-12
3. Effects of fibrinogen-binding tetrapeptides on mechanical properties of fine fibrin clots
4. Probing the basis of antibody reactivity with a panel of constrained peptide libraries displayed by filamentous phage
5. Encoded combinatorial chemistry
6. Identification of a biologically significant DNA-binding peptide motif by use of a random phage display library
7. Mimotopes identified by phage display for the monoclonal antibody CII-C1 to type II collagen
8. Epitope discovery using peptide libraries displayed on phage
9. Effects of amino acid substitutions at the active site in Escherichia coli beta-galactosidase
10. Crystal structure of fragment double-D from human fibrin with two different bound ligands
11. Effect of calcium and synthetic peptides on fibrin polymerization
12. Adhesive peptides selected by phage display: Characterization, applications and similarities with fibrinogen
13. Identification of polyclonal serum specificities with phage-display libraries
14. In vitro genetic analysis of the Tetrahymena self-splicing intron
15. The use of the synthetic peptide, Gly-Pro-Arg-Pro, in the preparation of thrombin-degranulated rabbit platelets
16. Whole genome PCR: Application to the identification of sequences bound by gene regulatory proteins
17. Rapid and efficient site-specific mutagenesis without phenotypic selection
18. Gly-Pro-Arg-Pro derivatives that bind to human plasma albumin and prevent fibrin formation
19. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
20. Identification and characterisation of C1q-binding phage displayed peptides
21. Replica plating and indirect selection of bacterial mutants: Isolation of preadaptive mutants in bacteria by sib selection
22. Cell-specific peptide binding by human neutrophils
23. Constrained peptide libraries as a tool for finding mimotopes
24. Two human neonatal IgM antibodies encoded by different variable-region genes bind the same linear peptide: Evidence for a stereotyped repertoire of epitope recognition
25. High affinity ligands from in vitro selection: Complex targets
26. The use of random-sequence oligonucleotides for determining consensus sequences
27. Molecular structure and intermolecular forces
28. Identification of peptide sequences that bind the Thomsen-Friedenreich cancer-associated glycoantigen from bacteriophage peptide display libraries
29. Method and means for sorting and identifying biological information pat: 5866363
30. Molecular heterogeneity of the vascular endothelium revealed by in vivo phage display
31. Phage display methods for selecting zinc finger proteins with novel DNA-binding specificities
32. Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA
33. Inhibition of human complement by a C3-binding peptide isolated from a phage-displayed random peptide library
34. Searching for peptide ligands with an epitope library
35. Isolation of cell surface-specific human monoclonal antibodies using phage display, and magnetically-activated cell sorting: Applications in immunohematology
36. Libraries of peptides and proteins displayed on filamentous phage
37. Cross-linked filamentous phage as an affinity matrix
38. Characterisation of epitopes on human p53 using phage-displayed peptide libraries: Insights into antibody-peptide interactions
39. Phage display selection on whole cells yields a peptide specific for melanocortin receptor 1
40. In vitro genetics
41. The epitopes for natural polyreactive antibodies are rich in proline
42. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
43. Subtractive isolation of phage-displayed single-chain antibodies to thymic stromal cells by using intact thymic fragments
44. The mathematics of SELEX against complex targets
45. High affinity type I interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries