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Volumn 122, Issue 20, 2000, Pages 5004-5005

Self-assembled double-stranded DNA (dsDNA) microarrays for protein:dsDNA screening using atomic force microscopy [13]

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA;

EID: 0034709385     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja000411f     Document Type: Letter
Times cited : (58)

References (20)
  • 1
    • 0343228093 scopus 로고    scopus 로고
    • C6 S-S, Glen Research
    • C6 S-S, Glen Research.
  • 4
    • 0342358538 scopus 로고    scopus 로고
    • note
    • 5′-Fluorescein phosphoramidite, 6-FAM, Glen Research.
  • 10
    • 0343663725 scopus 로고    scopus 로고
    • note
    • Oligonucleotides were exposed to an array formed after Step 3 for 2 h in 1 M THAM (tris(hydroxymethyl)aminomethane) (pH 7.4).
  • 12
    • 0343228086 scopus 로고    scopus 로고
    • note
    • 2) for 1 h.
  • 13
    • 0342793523 scopus 로고    scopus 로고
    • note
    • We estimate the density of dsDNA after MCH treatment to be 60% of its original value. Experiments are underway to more fully characterize oligonucleotide density.
  • 15
    • 0343228084 scopus 로고    scopus 로고
    • note
    • 2, and 50 mM NaCl) and placed on the array. Arrays were incubated in a dark, humid environment for 20 h at room temperature. Samples were then rinsed with deionized water and dried with argon. Next, 30 μL of 1 M THAM (pH 7.4) was placed on the array for 1 h at room temperature. Samples were again rinsed with deionized water and dried with argon prior to imaging.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.