Cytotoxic activity of recombinant bFGF-rViscumin fusion proteins

Biochemical and Biophysical Research Communications

Volumn 277, Issue 2, 2000, Pages 499-506

  Schmidt, Arno ^a   Möckel, Babette ^a   Eck, Jürgen ^a   Langer, Martin ^a   Gauert, Marc ^a   Zinke, Holger ^a  

^a BRAIN AG   (Germany)

Author keywords

B16 melanoma cells; Chimeric toxin; Fibroblast growth factor; Mitotoxin; Recombinant mistletoe lectin; Ribosome inactivating protein

Indexed keywords

HYBRID PROTEIN; IMMUNOTOXIN; RECOMBINANT BASIC FIBROBLAST GROWTH FACTOR; RECOMBINANT PROTEIN; RIBOSOME INACTIVATING PROTEIN; VISCUM ALBUM LECTIN;

ARTICLE; CYTOTOXICITY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; MELANOMA CELL; MOLECULAR CLONING; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ESCHERICHIA COLI; VISCUM ALBUM;

EID: 0034703612     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.3697     Document Type: Article

References (41)

1. Immunotoxin therapy
2. Recombinant immunotoxins: Protein engineering for cancer therapy
3. T cell-targeted immunofusion proteins from Escherichia coli
4. Recombinant single-chain immunotoxins against T and B cell leukemias
5. Evidence for a novel hybrid immunotoxin recognizing ricin A-chain by one antigen-combining site and a prostate-restricted antigen by the remaining antigen-combining site: Potential for immunotherapy
6. Bispecific F(ab’ gamma)2 antibody for the delivery of saporin in the treatment of lymphoma
7. Mitotoxins: Growth factor-targeted cytotoxic molecules
8. Influence of treatment with the immunomodulatory effective dose of the beta-galactoside-specific lectin from mistletoe on tumor colonization in BALB/c-mice for two experimental model systems
9. Effects of mistletoe lectin I on human blood cell lines and peripheral blood cells. Cytotoxicity, apoptosis and induction of cytokines
10. Ribosome-inactivating proteins from plants
11. Crystal structure of mistletoe lectin I from Viscum album
12. Ricin: Structure, mode of action, and some current applications
13. The site of action of six different ribosome-inactivating proteins from plants on eukaryotic ribosomes: The RNA N-glycosidase activity of the proteins
14. Site-specific mutagenesis of mistletoe lectin: The role of RIP activity in apoptosis
15. Entry of lethal doses of abrin, ricin and modeccin into the cytosol of HeLa cells
16. Importance of the antigen-binding valency and the nature of the cross-linking bond in ricin A-chain conjugates with antibody
17. Immunotoxins: Is there a clinical value?
18. Cytotoxicity of a recombinant ricin-A-chain fusion protein containing a proteolytically-cleavable spacer sequence
19. Biologically active interleukin 2-ricin A chain fusion proteins may require intracellular proteolytic cleavage to exhibit a cytotoxic effect
20. Cloning of the mistletoe lectin gene and characterization of the recombinant A-chain
21. Human basic fibroblast growth factor: Nucleotide sequence and genomic organization
22. Anti-B16-F10 melanoma activity of a basic fibroblast growth factor-saporin mitotoxin
23. The characterization of the receptor for endothelial cell growth factor by covalent ligand attachment
24. Bovine brain-derived growth factor. Purification and characterization of its interaction with responsive cells
25. Internalization and processing of basic fibroblast growth factor by neurons and astrocytes
26. Three-dimensional structure of human basic fibroblast growth factor, a structural homolog of interleukin 1 beta
27. The heparin-binding (fibroblast) growth factor family of proteins
28. Single step purification of biologically active recombinant rat basic fibroblast growth factor by immobilized metal affinity chromatography
29. Cysteine to serine substitutions in basic fibroblast growth factor: Effect on inclusion body formation and proteolytic susceptibility during in vitro refolding
30. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
31. Characterization of recombinant and plant-derived mistletoe lectin and their B-chains
32. Removal of a proteolytic activity associated with aggregates formed from expression of creatine kinase in Escherichia coli leads to improved recovery of active enzyme
33. A nonradioactive assay for ribosome-inactivating proteins
34. Systemic immunotoxin therapy of cancer: Advances and prospects
35. Pharmacokinetics of tumor-reactive immunotoxins in tumor-bearing mice: Effect of antibody valency and deglycosylation of the ricin A chain on clearance and tumor localization
36. Expression and activities of a recombinant basic fibroblast growth factor-saporin fusion protein
37. Synergy between immunotoxins prepared with native ricin A chains and chemically-modified ricin B chains
38. An immunotoxin prepared with blocked ricin: A natural plant toxin adapted for therapeutic use
39. Syndecan-1 is required for Wnt-1-induced mammary tumorigenesis in mice
40. Addition of an endoplasmic reticulum retrieval sequence to ricin A chain significantly increases its cytotoxicity to mammalian cells