On-column derivatization of oligodeoxynucleotides with ferrocene?

Chemical Communications

Volumn , Issue 6, 2000, Pages 509-510

  Beilstein, Amy E ^a   Grinstaff, Mark W ^a  

^a DUKE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FERROCENE; OLIGODEOXYNUCLEOTIDE DERIVATIVE;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL STRUCTURE; DRUG SYNTHESIS; MELTING POINT; NUCLEOTIDE METABOLISM; SOLID STATE;

EID: 0034696601     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/a907568b     Document Type: Article

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32. Interruption of DNA synthesis to perform the coupling reaction resulted in higher yields than synthesizing the oligonucleotide in entirety before coupling to FPA (50 cf. 8% yield).
33. The labeled ODNs were confirmed by ESI-MS in the negative mode (1: (M - 2)/2, (M - 3)/3 and (M - 4)/4 ions found, with a reconstructed mass of 5132.84, compared to the calculated molecular mass of 5131.187).
34. Equimolar amounts of complimentary single strands were combined to give a duplex solution of absorbance of ca. 0.5. The mixture was heated to 80 °C for 15 min and was then cooled to ambient temperature over 3 h. After cooling, the following parameters were used for the melting experiment: (a) monitoring wavelength, 260 nm; (b) temperature range, 20-70 °C; (c) temperature step, 0.5 °C; (d) integration time 1 s; and wait time 1.0 min. Melting temperatures were determined by the first derivative of the melting profile and were repeated multiply for each duplex.