Influence of hyperosmolar basal media on hybridoma cell growth and antibody production

Bioprocess Engineering

Volumn 16, Issue 6, 1997, Pages 305-310

  Ryu, J S ^a   Lee, G M ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ANTIBODY PRODUCTION; ARTICLE; CELL GROWTH; CONTROLLED STUDY; CULTURE MEDIUM; HYBRIDOMA; HYPEROSMOLARITY; MOUSE; NONHUMAN; OSMOTIC STRESS; PRIORITY JOURNAL;

EID: 0031006049     PISSN: 0178515X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004490050327     Document Type: Article

References (15)

1. Ozturk, S.S.; Palsson, B.O.: Effect of medium osmorality on hybridoma growth, metabolism, and antibody production. Biotechnol. Bioeng. 37 (1991) 989-993
2. Reddy, S; Bauer, K.D.; Miller, W.M.: Determination of antibody content in live versus dead hybridoma cells: Analysis of antibody production in osmotically stressed cultures. Biotechnol. Bioeng. 40 (1992) 947-964
3. Oh, S.K.W.; Vig, P.; Chua, F.; Teo, W.K.; Yap, M.G.S.: Substantial overproduction of antibodies by applying osmotic pressure and sodium butyrate. Biotechnol. Bioeng. 42 (1993) 601-610
4. Bibila, T.A.; Ranucci, C.S.; Glazomitsky, K.; Buckland, B.C.; Aunins, J.G.: Monoclonal antibody process development using medium concentrates. Biotechnol. Prog. 10 (1994) 87-96
5. Reddy, S.; Miller, W.M.: Effects of abrupt and gradual osmotic stress on antibody production and content in hybridoma cells that differ in production kinetics. Biotechnol. Prog. 10 (1994) 165-173
6. Øyaas, K.; Ellingsen, T.E.; Dyrset, N.; Levine, D.W.: Utilization of osmoprotective compounds by hybridoma cells exposed to hyperosmotic stress. Biotechnol. Bioeng. 43 (1994a) 77-89
7. Øyaas, K.; Ellingsen, T.E.; Dyrset, N.; Levine, D.W.: Hyperosmotic hybridoma cell cultures: Increased monoclonal antibody production with addition of glycine betaine. Biotechnol. Bioeng. 44 (1994b) 991-998
8. Park, S.Y.; Lee, G.M.: Feasibility study on the use of hyperosmolar medium for improved antibody production of hybridoma cells in a long-term, repeated-fed batch culture. Bioprocess Engr. 13 (1995a) 79-86
9. Lee, G.M.; Park, S.Y.: Enhanced specific antibody productivity of hybridomas resulting from hyperosmotic stress is cell line-specific. Biotechnol. Lett. 17 (1995) 145-150
10. Park, S.Y.; Lee, G.M.: Enhancement of monoclonal antibody production by immobilized hybridoma cell culture with hyperosmolar medium. Biotechnol. Bioeng. 48 (1995b) 699-705
11. Bergman, Y.; Haimovich, J.: Characterization of a carcinogen-induced murine B lymphocyte cell line of C3H/eb origin. Eur. J. Immunol. 7 (1977) 413-417
12. Schroer, J.A.; Bender, T.; Feldmann, R.J.; Kim, K.J.: Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur. J. Immunol. 13 (1983) 693-700
13. Lee, G.M.; Huard, T.K.; Palsson, B.O.: Effect of serum concentration on hybridoma cell growth and monoclonal antibody production at various initial cell densities. Hybridoma 8 (1989) 369-375
14. Lee, G.M.; Varma, A.; Palsson, B.O.: Production of monoclonal antibody using free-suspended and immobilized hybridoma cells: Effect of serum. Biotechnol. Bioeng. 38 (1991) 821-830
15. Lee, G.M.: Production of monoclonal antibody using free and immobilized hybridoma cells. Ph.D. thesis, University of Michigan, Ann Arbor, 1990