Flow cytometric analysis of intracellular IFN-γ, IL-4 and IL-10 in CD3+4+ T-cells from rat spleen

Journal of Immunological Methods

Volumn 244, Issue 1-2, 2000, Pages 29-40

  Caraher, Emma M ^a   Parenteau, Monique ^b   Gruber, Heidi ^b   Scott, Fraser W ^a,b  

^a OTTAWA HOSPITAL RESEARCH INSTITUTE   (Canada)

^b HEALTH CANADA   (Canada)

Author keywords

IFN ; IL 10; IL 4; Intracellular cytokine staining; Protein transport inhibitors; Rat spleen cells

Indexed keywords

BREFELDIN A; CALCIMYCIN; GAMMA INTERFERON; INTERLEUKIN 10; INTERLEUKIN 4; IONOMYCIN; MONENSIN; PHORBOL 13 ACETATE 12 MYRISTATE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; FLOW CYTOMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN TRANSPORT; RAT; SPLEEN CELL; STAINING; T LYMPHOCYTE;

ANIMAL; ANTIGENS, CD3; BREFELDIN A; FLOW CYTOMETRY; INTERFERON TYPE II; INTERLEUKIN-10; INTERLEUKIN-4; IONOMYCIN; IONOPHORES; LYMPHOCYTE ACTIVATION; MONENSIN; PROTEIN SYNTHESIS INHIBITORS; RATS; RATS, INBRED BB; SPLEEN; STAINING AND LABELING; SUPPORT, NON-U.S. GOV'T; T-LYMPHOCYTES; TETRADECANOYLPHORBOL ACETATE;

EID: 0034693359     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(00)00249-0     Document Type: Article

References (30)

1. Regulation of CD4 and CD8 expression on mouse T cells. Active removal from the cell surface by two mechanisms
2. Simultaneous production of interleukin 2, interleukin 4 and interferon-gamma by activated human blood lymphocytes
3. Flow cytometric determination of cytokines in activated murine T helper lymphocytes: expression of interleukin-10 in interferon-gamma and in interleukin-4-expressing cells
4. Differential production of intracellular gamma interferon in alpha beta and gamma delta T-cell subpopulations in response to peritonitis
5. Flow cytometric analysis of intracellular interferon-gamma synthesis in rat CD4 T cells
6. Immunofluorescent staining of intracellular cytokines for flow cytometric analysis
7. Phorbol myristate acetate-induced down-modulation of CD4 is dependent on calmodulin and intracellular calcium
8. GolgiPlug (brefeldin A) vs. GolgiStop (monensin) for intracellular cytokine staining
9. Detection of altered T helper 1 and T helper 2 cytokine production by peripheral blood mononuclear cells in patients with multiple sclerosis utilizing intracellular cytokine detection by flow cytometry and surface marker analysis
10. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+CD27-lymphocyte subpopulation
11. Detection of intracellular cytokines by flow cytometry
12. Normal T-helper 1/T-helper 2 balance in peripheral blood of coeliac disease patients
13. Lymphocyte subsets in distinct lung compartments show a different ability to produce interferon-gamma (IFN-gamma) during a pulmonary immune response
14. Generation of interleukin 4 (IL-4)-producing cells in vivo and in vitro: IL-2 and IL-4 are required for in vitro generation of IL-4-producing cells
15. Detecting cytokine production at the single-cell level
16. A simultaneous three-color T cell subsets analysis with single laser flow cytometers using T cell gating protocol. Comparison with conventional two-color immunophenotyping method
17. Expression and kinetics of cytokines determined by intracellular staining using flow cytometry
18. TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties
19. Brefeldin A, but not monensin, completely blocks CD69 expression on mouse lymphocytes: efficacy of inhibitors of protein secretion in protocols for intracellular cytokine staining by flow cytometry
20. Quantitative intracellular cytokine measurement: age-related changes in proinflammatory cytokine production
21. Cytokine flow cytometry: understanding cytokine biology at the single-cell level
22. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies
23. An update on cytokines in the pathogenesis of insulin-dependent diabetes mellitus
24. Kinetics of intracytoplasmic Th1 and Th2 cytokine production assessed by flow cytometry following in vitro activation of peripheral blood mononuclear cells
25. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure
26. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining
27. Determination of intracellular cytokines by flow-cytometry following whole-blood culture
28. IL-4 directs the development of Th2-like helper effectors
29. Reduction in interleukin-2-producing cells but not Th1 to Th2 shift in moderate and advanced stages of human immunodeficiency virus type-1-infection: direct analysis of intracellular cytokine concentrations in CD4+ CD8-T cells
30. Age-related alteration of cytokine production profile by T cell subsets in mice: a flow cytometric study