Effect of variation of Klebsiella pneumoniae acetolactate synthase expression on metabolic flux redistribution in Escherichia coli

Biotechnology and Bioengineering

Volumn 69, Issue 2, 2000, Pages 150-159

  Yang, Yea Tyng ^a   Peredelchuk, M ^a   Bennett, George N ^a   San, Ka Yiu ^a  

^a RICE UNIVERSITY   (United States)

Author keywords

Acetolactate synthase (ALS); Escherichia coli; Klebsiella pneumoniae; Metabolic flux distribution; Sensitivity analysis

Indexed keywords

CATALYSIS; CATALYST ACTIVITY; CHEMOSTATS; COMPOSITION EFFECTS; ENZYMES; GENES; METABOLISM; PHOSPHATES;

ACETOLACTATE SYNTHASE; ADENOSINE TRIPHOSPHATE; METABOLIC FLUX ANALYSIS;

ESCHERICHIA COLI;

ACETOIN; ACETOLACTATE SYNTHASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INDUCTION; ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE; NONHUMAN; PH; PROTEIN EXPRESSION;

ACETOLACTATE SYNTHASE; BIOREACTORS; BIOTECHNOLOGY; ENZYME INDUCTION; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; HYDROGEN-ION CONCENTRATION; INDOLEACETIC ACIDS; KINETICS; KLEBSIELLA PNEUMONIAE; RECOMBINANT PROTEINS;

BACILLUS SUBTILIS; ESCHERICHIA COLI; KLEBSIELLA; KLEBSIELLA PNEUMONIAE;

EID: 0034691711     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(20000720)69:2<150::AID-BIT4>3.0.CO;2-N     Document Type: Article

References (41)

1. Aristidou AA, San K-Y, Bennett GN. 1994. Modification of central metabolic pathway in Escherichia coli to reduce acetate accumulation by heterologous expression of the Bacillus subtilis acetolactate synthase gene. Biotechnol Bioeng 44:944-951.
2. Aristidou AA, San K-Y, Bennett GN. 1999. Metabolic flux analysis of E. coli expressing the B. subtilis ALS in batch and continuous cultures. Biotechnol Bioeng 63:737-749.
3. Barak Z, Chipman DM, Schloss JV. 1988. Biosynthesis of branched chain amino acids. In: Workshop on the Biosynthesis of Branched Chain Amino Acids, Beer-Sheva, Israel, Palaban: VCH.
4. Barak Z, Kogan N, Gollop N, Chipman DM. 1988. Importance of AHAS isozymes in branched chain amino acid biosynthesis. In: Biosynthesis of branched chain amino acids. Weinheim: VCH. 91-107.
5. Chou C-H, Bennett GN, San K-Y. 1994. Effect of modified glucose uptake using genetic engineering techniques on high-level recombinant protein production in Escherichia coli dense cultures. Biotechnol Bioeng 44:952-960.
6. Eddy BP. 1961. The Voges-Proskauer reaction and its significance: a review. J Appl Bacteriol 24:27-41.
7. Goldbeter A, Koshland D. 1982. Sensitivity amplification in biochemical systems. Q Rev Biophys 15:555-591.
8. Harris RA, Sokatch JR. 1985. Branched-chain amino acids. New York: Academic Press.
9. Holtzclaw WD, Chapman LF. 1975. Degrative acetolactate synthase of Bacillus subtilis: purification and properties. J Bacteriol 121:917-922.
10. Ingraham JL, Malloe O, Neidhardt FC. 1983. Growth of the bacterial cell. Sunderland, MA: Sinauer.
11. Iuchi S. 1993. Phosphorylation/dephosphorylation of the receiver module at the conserved aspartate residue controls transphosphorylation activity of histidine kinase in sensor protein ArcB of Escherichia coli. J Biol Chem 263:23972-23980.
12. Iuchi S, Lin ECC. 1992. Mutational analysis of signal transduction by ArcB: a membrane sensor protein for anaerobic expression of operons involved in the central aerobic pathways in Escherichia coli. J Bacteriol 174:3972-3980.
13. Kaiser M, Sawers G. 1994. Pyruvate formate-lyase is not essential for nitrate respiration by Escherichia coli. FEMS Microbiol Lett 117: 163-168.
14. Kaiser M, Sawers G. 1997. Overlapping promoters modulate Fnr- and ArcA-dependent anaerobic transcriptional activation of the focApfl operon in Escherichia coli. Microbiology 143:775-783.
15. Kawai S, Mizutani S, IijimaS, Kobayashi T. 1986. On-off regulation of the tryptophan promoter in fed-batch culture. J Ferment Technol 64: 503-510.
16. Kessler D, Herth W, Knappe J. 1992. Ultrastructure and pyruvate formate-lyase radical quenching property of the multienzymic adhE protein of Escherichia coli. J Biol Chem 267:18073-18079.
17. Knappe J, Schacht J, Mochel W, Hopner T, Vetter J, Edenharder R. 1969. Pyruvate formate-lyase reaction Escherichia coli. Eur J Biochem 11:316-327.
18. Lin ECC, Iuchi S. 1994. Role of protein phosphorylation in the regulation of aerobic metabolism by the Arc system in Escherichia coli. In: Torriani-Gorini A, Yagil E, Silver S, editors. Phosphate in microorganisms: cellular and molecular biology. Washington, DC: American Society for Microbiology. p 290-295.
19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265-275.
20. Lynch AS, Lin ECC. 1996. Responses to molecular oxygen. In: Neidhardt F, Curtis III R, Ingraham JL, Lin ECC, Low KB, Magasnik B, Reznikoff WS, Riley M, Schaechter M, Umbarger HE, editors. Escherichia coli and Salmonella: cellular and molecular biology. Washington, DC. American Society for Microbiology. p 1526-1538.
21. Padan E, Schuldiner S. 1987. Intracellular pH and membrane potential as regulators in the prokaryotic cell. J Membr Biol 95:189-198.
22. Padan E, Zilberstein D, Schuldiner S. 1981. pH homeostasis in bacteria. Biochem Biophys Acta 650:151-166.
23. Park TH, Seo J-H, Lim HC. 1989. Analysis of kinetic parameters for the 3β-indoleacrylic acid effect on trp promoter in Escherichia coli. Biotechnol Lett 11:87-92.
24. Peng H-L, Wang P-Y, Wu C-M, Hwang D-C, Chang H-Y. 1992. Cloning, sequencing and heterologous expression of a Klebsiella pneumoniae gene encoding an FAD-independent acetolactate synthase. Gene 17: 125-130.
25. Sawers G. 1993. Specific transcriptional requirements for positive regulation of the anaerobically inducible pfl operon by ArcA and FNR. Mol Microbiol 10:737-747.
26. Sawers G, Böck A. 1988. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12. J Bacteriol 170:5330-5336.
27. Sawers G, Böck A. 1989. Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression. J Bacteriol 171:2485-2498.
28. Sawers G, Suppman B. 1992. Anaerobic induction of pyruvale formate-lyase gene expression is mediated by the ArcA and FNR proteins. J Bacteriol 174:3474-3478.
29. 70-dependent transcription in vitro and heterologous expression in Pseudomonas putida in vivo. J Bacteriol 171:4930-4937.
30. Small JR, Kacser H. 1993. Responses of metabolic systems to large changes in enzyme activities and effectors: 1. The linear treatment for unbranched chains. Eur J Biochem 213:613-624.
31. Spencer ME, Guest JR. 1985. Transcription analysis of the sucAB, aceEF and lpd genes of Escherichia coli. Mol Gen Genet 200:145-154.
32. Störmer FC. 1968a. Evidence for induction of the 2,3-butanediol-forming enzymes in Aerobacter aerogenes. FEBS Lett 2:36-38.
33. Störmer FC. 1968b. The pH 6 acetolactate-forming enzyme form Aerobacter aerogenes. I. Kinetic studies. J Biol Chem 243:3735-3739.
34. Störmer FC. 1975. 2,3-Butanediol biosynthetic system in Aerobacter aerogenes. In: Carbohydrate metabolism. Part B. New York: Academic Press. p 518-533.
35. Tarmy EM, Kaplan NO. 1968. Kinetics of Escherichia coli B D-lactate dehydrogenase and evidence for pyruvate-controlled change in conformation. J Biol Chem 243:2587-2596.
36. Tolentino GJ, Meng S-Y, Bennett GN, San K-Y. 1992. A pH-regulated promoter for the expression of recombinant proteins in Escherichia coli. Biotechnol Lett 14:157-162.
37. Umbarger HE. 1978. Amino acids biosynthesis and its regulation. Ann Rev Biochem 47:533-606.
38. Westerfeld WW. 1945. A calorimetric determination of blood acetoin. J Biol Chem 161:495-502.
39. Yang Y-T, San K-Y, Bennett GN. 1999a. Metabolic flux analysis of Escherichia coli deficient in the acetate production pathway and expressing the Bacillus subtilis acetolactate synthase. Metab Eng 1:26-34.
40. Yang Y-T, San K-Y, Bennett GN. 1999b. Redistribution of metabolic fluxes in Escherichia coli with fermentative lactate dehydrogenase overexpression and deletion. Metab Eng 1:141-152.
41. Yansura DG, Henner JH. 1990. Use of Escherichia coli trp promoter for direct expression of proteins. Meth Enzymol 185:54-60.