Homogeneous, nanoparticle-based quantitative colorimetric detection of oligonucleotides [13]

Journal of the American Chemical Society

Volumn 122, Issue 15, 2000, Pages 3795-3796

  Reynolds III, Robert A ^a   Mirkin, Chad A ^a   Letsinger, Robert L ^b  

^a NORTHWESTERN UNIVERSITY   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

EPIANDROSTERONE; GOLD; NANOPARTICLE; OLIGONUCLEOTIDE;

COLORIMETRY; HYBRIDIZATION; LETTER; MOLECULAR STABILITY; POLYMERASE CHAIN REACTION; QUANTITATIVE ASSAY; SEDIMENTATION RATE; ULTRAVIOLET SPECTROSCOPY;

EID: 0034685510     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja000133k     Document Type: Letter

References (25)

1. (a) Hames, B. D.; Higgins, S. J., Eds. Gene Probes 1; IRL Press: New York, 1995.
2. (b) Kricka, L. J., Ed. Nonisotopic DNA Probe Techniques; Academic Press: San Diego, 1992.
3. (c) Lee, L.; Connell, C.; Bloch, W. Nucleic Acid Res. 1993, 21, 3761-3766.
4. Keller, G. H.; Manak, M. M., Eds. DNA Probes; Stockton Press: New York, 1989.
5. Heid, C. A.; Stevens, J.; Livak, K. J.; Williams, P. M. Genome Res. 1996, 6, 986-994
6. Lee, L.; Connell, C.; Bloch, W. Nucleic Acid Res. 1993, 21, 3761-3766.
7. (a) Tyagi, S.; Kramer, F. R. Nature Biotechnol. 1996, 14, 303-308.
8. (b) Tyagi, S.; Bratu, D. P.; Kramer, F. R. Nature Biotechnol. 1998, 16, 49-53.
9. (c) Piatek, A. S.; Tyagi, S.; Pol, A. C.; Telenti, A.; Miller, L. P.; Kramer, F. R.; Alland, D. Nature Biotechnol. 1998, 16, 359-363.
10. (a) Mirkin, C. A.; Storhoff, J. J. Chem. Rev. 1999, 99, 1849-1862.
11. (b) Storhoff, J. J.; Elghanian, R.; Mucic, R. C.; Mirkin, C. A.; Letsinger, R. L. J. Am. Chem. Soc. 1998, 120, 1959-1964.
12. (c) Elghanian, R.; Storhoff, J. J.; Mucic, R. C.; Letsinger, R. L.; Mirkin, C. A. Science 1997, 227, 1078-1081.
13. (d) Mirkin, C. A.; Letsinger, R. L.; Mucic, R. C.; Storhoff, J. J. Nature 1996, 382, 607-609.
14. Letsinger, R. L.; Elghanian, R.; Viswanadham, G.; Mirkin, C. A. Bioconjugate Chem. 2000, 11, 289.
15. Yguerabide, J.; Yguerabide, E. E. Anal. Biochem. 1998, 262, 137-156.
16. (a) Gold nanoparticles (50 and 100 nm) were purchased from Vector Labs, Burlingame CA. The actual particle sizes are 51.9 ± 3.5 and 99.5 ± 4.5 nm as determined by the supplier via TEM. (b) The oligonucleotide modification procedures for the 50 and 100 gold particles with dithiane androsterone-functionalized oligonucleotides have been deposited as Supporting Information.
17. Storhoff, J. J.; Lazarides, A. A.; Mirkin, C. A.; Letsinger, R. L.; Mucic, R. C.; Schatz, G. C. J. Am. Chem. Soc., in press.
18. 20-ATC-CTT-ATC-AAT-ATT-3′. The 15 base recognition sequences are complementary to a 30 base section of a PCR product from Bacillus anthracis, the 141 base pair anthrax protective antigen.
19. 5′-GAG-GGA-TTA-TTG-TTA-AAT-ATT-GAT-AAG-GAT-3′.
20. All hybridization experiments were performed in a buffer solution of 0.3 M NaCl, 10 mM phosphate pH 7.0, and 0.01% (w/w) sodium dodecyl sulfate.
21. Thermal denaturation profiles of 50 and 100 nm gold particle aggregates have been deposited as Supporting Information.
22. Full width at half-maximum.
23. max of 520, 540, and 580 nm prior to aggregation; for 13, 50, and 100 nm gold nanoparticles (2 nM, 35 pM, and 3.8 pM, respectively) and a target concentration of 500 pM were used for the melting studies. Prior to melting these solutions were annealed at 40 °C for 2 h.
24. This was verified by dynamic light scattering and TEM (Supporting Information) 2 h post target addition, through the comparison of a 1:1 to a 5:1 ratio of gold nanoparticle probe solutions. The solutions were ∼30 pM in gold nanoparticle probe and 500 pM in target oligonucleotide. The 1:1 ratio of probes had an effective aggregate diameter of ∼300 nm after 60 min, while the 5:1 ratio of probes had an effective diameter of only 130 nm.
25. The total concentration of gold probes was 30 pM.