Co-expression of the Bordetella pertussis leader peptidase I results in enhanced processing and expression of the pertussis toxin S1 subunit in Escherichia coli

FEMS Microbiology Letters

Volumn 191, Issue 2, 2000, Pages 177-182

  Smith, Adam M ^a   Yan, Huaru ^a   Groves, Nicola ^a   Dalla Pozza, Tania ^a   Walker, Mark J ^a  

^a UNIVERSITY OF WOLLONGONG   (Australia)

Author keywords

Leader peptide; Leader sequence; Signal peptidase; Signal peptide; Whooping cough

Indexed keywords

PERTUSSIS TOXIN; PERTUSSIS VACCINE; SIGNAL PEPTIDASE; SIGNAL PEPTIDE;

ARTICLE; BORDETELLA PERTUSSIS; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PERTUSSIS; PRIORITY JOURNAL; PROTEIN PROCESSING; VACCINE PRODUCTION;

AMINO ACID SEQUENCE; BORDETELLA PERTUSSIS; CLONING, MOLECULAR; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES; SUPPORT, NON-U.S. GOV'T; VIRULENCE FACTORS, BORDETELLA;

EID: 0034668290     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00386-4     Document Type: Article

References (32)

1. Greco D., et al. A controlled trial of two acellular vaccines and one whole cell vaccine against pertussis. N. Engl. J. Med. 334:1996;341-348.
2. Gustafsson L., Hallander H.O., Olin P., Reizenstein E., Storsaeter J. A controlled trial of a two-component acellular, a five component acellular and a whole cell pertussis vaccine. N. Engl. J. Med. 334:1996;349-355.
3. Nicosia A., Bartoloni A., Perugini M., Rappuoli R. Expression and immunological properties of the five subunits of pertussis toxin. Infect. Immun. 55:1987;963-967.
4. Burnette N., Mar V.L., Cieplak W., Kaljot K.T., Marchitto K.S., Sachdev R.K., Locht C., Keith J.M. Direct expression of B. pertussis toxin subunits to high levels in E. coli. Bio/technology. 6:1988;699-706.
5. Dalla Pozza T., Yan H., Walker M.J. Expression of a synthetic pertussis toxin operon in Escherichia coli. Vaccine. 15:1997;968-975.
6. Dalbey R.E., Wickner W. Leader peptidase catalyzes the release of exported proteins from the outer surface of the Escherichia coli plasma membrane. J. Biol. Chem. 260:1985;15925-15931.
7. Randall L.L., Hardy S.J.S., Thom J.R. Export of protein: a biochemical view. Ann. Rev. Microbiol. 41:1987;507-541.
8. Munoa F.J., Miller K.W., Beers R., Graham M., Wu H.C. Membrane topology of Escherichia coli prolipoprotein signal peptidase (signal peptidase II). J. Biol. Chem. 266:1991;17667-17672.
9. Nunn D., Lory S. Product of the Pseudomonas aeruginosa gene pilD is a prepilin leader peptidase. Proc. Natl. Acad. Sci. USA. 88:1991;3281-3285.
10. Wolfe P.B., Zwizinski C., Wickner W. Purification and characterization of leader peptidase from Escherichia coli. Methods Enzymol. 97:1983;40-46.
11. Date T., Wickner W. Isolation of the Escherichia coli leader peptidase gene and effects of leader peptidase overproduction in vivo. Proc. Natl. Acad. Sci. USA. 78:1981;6106-6110.
12. Zwizinski C., Wickner W. Purification and characterization of leader (signal) peptidase from Escherichia coli. J. Biol. Chem. 255:1980;7973-7977.
13. Black M.T., Munn J.G., Allsop A.E. On the catalytic mechanism of prokaryotic leader peptidase I. Biochem. J. 282:1992;539-543.
14. Cregg K.M., Wilding I., Black M.T. Molecular cloning and expression of the spsB gene encoding an essential type I signal peptidase from Staphylococcus aureus. J. Bacteriol. 178:1996;5712-5718.
15. Jock C.A., Pulakat L., Lee S., Gavini N. Nucleotide sequence and genetic complementation analysis of lep from Azotobacter vinelandii. Biochem. Biophys. Res. Commun. 239:1997;393-400.
16. Klug G., Jager A., Heck C., Rauhut R. Identification, sequence analysis, and expression of the lepB gene for a leader peptidase in Rhodobacter capsulatus. Mol. Gen. Genet. 253:1997;666-673.
17. Packer J.C., Andre D., Howe C.J. Cloning and sequence analysis of a signal peptidase I from the thermophilic cyanobacterium Phormidium laminosum. Plant Mol. Biol. 27:1995;199-204.
18. Wolfe P.B., Wickner W., Goodman J.M. Sequence of the leader peptidase gene of Escherichia coli and the orientation of leader peptidase in the bacterial envelope. J. Biol. Chem. 258:1983;12073-12080.
19. Bilgin N., Lee J.I., Zhu H.-Y., Dalbey R., von Heinje G. Mapping of catalytically important domains in Escherichia coli leader peptidase. EMBO J. 9:1990;2712-2722.
20. Walker M.J., Birch R.G., Pemberton J.M. Cloning and characterisation of an albicidin resistance gene from Klebsiella oxytoca. Mol. Microbiol. 2:1988;443-454.
21. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning. Cold Spring Harbor Press, New York.
22. Stainer D., Scholte M. A simple chemically defined medium for the production of phase I Bordetella pertussis. J. Gen. Microbiol. 63:1970;211-220.
23. Kasuga T., Nakase Y., Ukishima K., Takasu K. Studies on Haemophilus pertussis. Kitasato Arch. Exp. Med. 27:1954;57-62.
24. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 11:1980;291-298.
25. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
26. Burnette W.N. 'Western blotting': electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112:1981;195-203.
27. Kyte J., Doolittle R.F. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:1982;105-132.
28. Dalbey R.E. ASM News. 61:1995;586-590.
29. Monack D., Arico B., Rappouli R., Falkow S. Phase variants of Bordetella bronchiseptica arise by spontaneous deletions in the vir locus. Mol. Microbiol. 3:1989;1719-1728.
30. Keen N.T., Tamaki S., Kobayashi D., Trollinger D. Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene. 70:1988;191-197.
31. Yanisch-Perron C., Vieria J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.
32. Walker M.J., Rohde M., Wehland J., Timmis K.N. Construction of minitransposons for constitutive and inducible expression of pertussis toxin in bvg-negative Bordetella bronchiseptica. Infect. Immun. 59:1991;4238-4248.