Expression of a synthetic pertussis toxin operon in Escherichia coli

Vaccine

Volumn 15, Issue 9, 1997, Pages 968-975

  Pozza, Tania Dalla ^a   Yan, Huaru ^b   Walker, Mark J ^b  

^a UNIVERSITY OF WOLLONGONG   (Australia)

^b NONE

Author keywords

Bordetella pertussis; Expression; Pertussis toxin; Vaccine

Indexed keywords

PERTUSSIS TOXIN; PERTUSSIS VACCINE;

ARTICLE; BORDETELLA PERTUSSIS; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; OPERON; PRIORITY JOURNAL; VIRULENCE;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; GENES, SYNTHETIC; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; OPERON; PEPTIDE BIOSYNTHESIS; PERTUSSIS TOXIN; RABBITS; RECOMBINANT PROTEINS; VIRULENCE FACTORS, BORDETELLA;

EID: 0031170933     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0264-410X(96)00306-4     Document Type: Article

References (38)

1. 1 Robinson, A., Irons, L.I. and Ashworth, L.A.E. Pertussis vaccine: present status and future prospects. Vaccine 1985, 3, 11
2. 2 Tarnura, M., Nagiman, L. and Murau, S. et al. Subunit structure of islet activating protein in pertussis toxin in the conformity with the A-B model. Biochemistry 1982, 21, 5516
3. 3 Locht, C. and Keith, J.M. Pertussis toxin gene: nucleotide sequence and genetic organisation. Science 1986, 232, 1258
4. 4 Nicosia, A., Perugini, M. and Franzini, C. et al. Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication. Proc. Natl Acad. Sci. USA 1986, 83, 4631
5. 5 Nicosia, A. and Rappuoli, R. Promoter of the pertussis toxin operon and production of pertussis toxin. J. Bacteriol. 1987, 169, 2843
6. 6 Weiss, A.A., Johnson, F.D. and Burns, D.L. Molecular characterisation of an operon required for pertussis toxin secretion. Proc. Natl Acad. Sci. USA 1993, 90, 2970
7. 7 Covacci, A. and Rappuoli, R. Pertussis toxin export requires accessory genes located drownstream from the pertussis toxin operon. Mol. Microbiol. 1993, 8, 429
8. 8 Johnson, F.D. and Burns, D.L. Detection and subcellular location of three Ptl proteins involved in the secretion of pertussis toxin from Bordetella pertussis. J. Bacteriol. 1994, 176, 5350
9. 9 Smith, A.M. and Walker, M.J. Transfer of a pertussis toxin expression locus to isogenic bvg-positive and bvg-negative strains of Bordetella bronchiseptica using an in vivo technique. Microbiol. Pathogen. 1996, 20, 263
10. 10 Walker, M.J., Rhode, M., Wehland, J. and Timmis, K.N. Construction of minitransposons for constitutive and inducible expression of pertussis toxin in Bvg negative B. bronchiseptica. Infect. Immun. 1991, 59, 4238
11. 11 Burnette, N., Mar, V.L. and Cieplak, W. et al. Direct expression of B. pertussis toxin subunits to high levels in E. coli. Bio/ technology 1988, 6, 699
12. 12 Necioni, L., Pizza, M.G. and Bugnoli, M. et al. Characterization of genetically inactivated pertussis toxin mutants: candidates for a new vaccine against whooping cough. Infect. Immun. 1990, 58, 1308
13. 13 Burnette, W.N., Arciniega, J.L., Mar, V.L. and Burns, D.L. Properties of pertussis toxin B oligomer assembled in vitro from recombinant polypeptides produced by Escherichia coli. Infect. Immun. 1992, 60, 2252
14. 14 Yanisch-Perron, C., Vieria, J. and Messing, J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 1985, 33, 103
15. 15 Mizushima, S. and Yamada, H. Isolation and characterisation of two outer membrane preparations from Escherichia coli. Biochim. Biophys. Acta 1975, 375, 44
16. 16 Kasuga, T., Nakase, Y., Ukishima, K. and Takasu, K. Studies on Haemophilus pertussis. Kitasato Arch. Exp. Med. 1954, 27, 57
17. 17 Sambrook, J., Fritsch, E F. and Maniatis, T. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, New York, 1989
18. 18 Walker, M. J. and Pemberton, J.M. Construction of transposons encoding genes for β-glucosidase, amylase and polygalacturonate trans-eliminase from Klebsiella oxytoca and their expression in a range of gram-negative bacteria. Curr. Microbiol. 1988, 17, 69
19. 19 Walker, M.J., Rohde, M., Timmis, K.T. and Guzman, C. Specific lung mucosal and systemic immune responses after oral immunisation of mice with Salmonella typhimurium aroA, Salmonella typhi Ty21a, and invasive Escherichia coli expressing recombinant pertussis toxin S1 subunit. Infect. Immun. 1992, 60, 4260
20. 20 Guzman, C.A., Piatti, G., Walker, M.J., Guardati, M.C. and Pruzzo, C. A Novel Escherichia coli expression-export vector containing alkaline phosphatase as an insertional inactivation screening system. Gene 1994, 148, 171
21. 21 Schauder, B., Blocker, H., Frank, R. and McCarthy, J.E.G. Inducible expression vectors incorporating the Escherichia coli atpE translational initiation region. Gene 1987, 52, 279
22. 22 Sanger, F., Nicklen, S. and Coulsen, R.R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 1977, 74, 5463
23. 23 Walker, M.J., Wehland, J., Timmis, K.T., Raupach, B. and Schmidt, M.A. Characterisation of murine monoclonal antibodies that recognize defined epitopes of pertussis toxin and neutralize its toxic effect on Chinese hamster ovary cells. Infect. Immun. 1991, 59, 4249
24. 24 Witviet, M., Burns, D.L., Brennan, M.J., Poolman, J.T. and Manclark, C.R. Binding of pertussis toxin to eucaryotic cells and glycoproteins. Infect. Immun. 1989, 57, 3324
25. 25 Bigio, M., Rossi, R. and Nucci, D. et al. Monoclonal antibodies against pertussis toxin subunits. FEMS Microbiol. Lett. 1988, 51, 7
26. 26 Lee, A.C.J., Powell, J.E., Tregear, G.W., Niall, H.D. and Stevens, V.C. A method for preparing β-hCG COOH peptide-carrier conjugates of predictable composition. Mol. Immunol. 1980, 17, 749
27. 27 Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680
28. 28 Burnette, W.N. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 1981, 112, 195
29. 29 Cornelis, P., Digneffe, C. and Willemot, K. Cloning and expression of a Bacillus coagulans amylase gene in Escherichia coli. Molec Gen. Genetics 1982, 186, 507
30. 30 McCarthy, J.E.G. and Bokelmann, C. Determinants of translational efficiency in the atp operon of Escherichia coli. Molec. Microbiol. 1988, 2, 455
31. 31 Schauder, B. and McCarthy, J.E.G, The role of bases upstream of the Shine-Dalgarno region and in the coding sequence in the control of gene expression in Escherichia coli: translational and stability of mRNAs in vivo. Gene 1989, 78, 59
32. 32 Wülfing, C. and Plückthun, A. Protein folding in the periplasm of Escherichia coli. Molec. Microbiol. 1994, 12, 685
33. 33 Kirn, Y.T., Muramatsu, T. and Takahashi, K. Leader peptidase from Escherichia coli-overexpression, characterization and inactivation by modification of tryptophan residues 300 and 310 with N-bromosuccinimide. J. Biochem. 1995, 117, 535
34. 34 Fraipont, C., Adam, M. and Nguyen-Disteche, M. et al. Engineering and overexpression of periplasmic forms of the penicillin-binding protein 3 of Escherichia coli. Biochem. J. 1994, 15, 189
35. 35 Perez-Perez, J., Marquez, G., Barbero, J.-L. and Gutierrez, J. Increasing the efficiency of protein export in Escherichia coli. Bio/technology 1994, 12, 178
36. 36 Hirst, R.T. and Holmgren, J. Transient entry of entertoxin subunits into the periplasm occurs during their secretion from Vibrio cholerae. J. Bacteriol. 1987, 169, 1037
37. 37 Kotob, S.I., Hausman, S.Z. and Burns, D.L. Localization of the promoter for the genes of Bordetella pertussis, which encodes proteins essential for secretion of pertussis toxin. Infect. Immun. 1995, 58, 1308
38. 38 Ricco, S., Rappuoli, R. and Scarlato, V. The pertussis toxin liberation genes of Bordetella pertussis are transcriptionally linked to the pertussis toxin operon. Infect Immun. 1996, 64, 1458