Differential use of Alcian blue and toluidine blue dyes for the quantification and isolation of anionic glycoconjugates from cell cultures: Application to proteoglycans and a high-molecular-weight glycoprotein synthesized by articular chondrocytes

Analytical Biochemistry

Volumn 285, Issue 2, 2000, Pages 211-219

  Terry, D E ^a   Chopra, R K ^a   Ovenden, J ^a   Anastassiades, T P ^a  

^a QUEEN S UNIVERSITY   (Canada)

Author keywords

Alcian blue; Chondrocytes; Glycoconjugates; Proteoglycans; Tissue culture; Toluidine blue

Indexed keywords

BIOLOGICAL MATERIALS; CARBOHYDRATES; CELLULOSE; DISSOCIATION; GLYCOPROTEINS; MOLECULAR WEIGHT; PRECIPITATION (CHEMICAL); RADIOGRAPHY; SCREENING; SULFUR COMPOUNDS; TISSUE CULTURE;

ALCIAN BLUES; CHONDROCYTES; GLYCOCONJUGATES; PROTEOGLYCANS; TOLUIDINE BLUE;

SILVER COMPOUNDS;

ALCIAN BLUE; CELLULOSE ACETATE; GLUCOSAMINE; GLYCOCONJUGATE; GLYCOPROTEIN; PROTEOGLYCAN; SILVER NITRATE; SULFATE; TOLONIUM CHLORIDE;

ANIMAL CELL; ARTICLE; AUTORADIOGRAPHY; CARTILAGE CELL; CATTLE; DISSOCIATION; ELECTROPHORETIC MOBILITY; LIQUID SCINTILLATION COUNTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; STAINING;

ANIMALIA; BOS TAURUS;

EID: 0034668082     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4761     Document Type: Article

References (21)

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4. The quantitative measurement of Alcian blue-glycosaminoglycan complexes
5. Quantitation and interaction of glycosaminoglycans with Alcian blue in dimethyl sulfoxide solutions
6. 2 concentrations
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8. Detection and quantitation of proteoglycans extracted from cell culture medium and cultured cartilage slices
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11. Simultaneous preparation and quantitation of proteoglycans by precipitation with Alcian blue
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14. Acid polysaccharide content of frog rod outer segments determined by metachromatic toluidine blue staining
15. Toluidine blue staining of cartilage proteoglycan subunits
16. The use of brominated toluidine blue O in X-ray microanalysis for proteoglycan
17. Differential effects of bone associated factors on newly synthesized anionic glycoconjugates by articular chondrocyte cultures from adult and immature bovines
18. Assembly of newly synthesized proteoglycan and link protein into aggregates in cultures of chondrosarcoma chondrocytes
19. Specificity and synergism of polypeptide growth factors in stimulating the synthesis of proteoglycans and a novel high molecular weight anionic glycoprotein by articular chondrocyte cultures