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Volumn 2, Issue 12, 2000, Pages 1693-1696

Synthesis of aminoglycoside-modified oligonucleotides

Author keywords

[No Author keywords available]

Indexed keywords

AMINOGLYCOSIDE; DNA; OLIGONUCLEOTIDE; RNA;

EID: 0034658925     PISSN: 15237060     EISSN: None     Source Type: Journal    
DOI: 10.1021/ol005797d     Document Type: Article
Times cited : (28)

References (21)
  • 12
    • 85037516218 scopus 로고    scopus 로고
    • note
    • 13C NMR, and mass spectroscopy.
  • 14
    • 85037510987 scopus 로고    scopus 로고
    • note
    • The mixture of these two compounds could only be separated by silica gel chromatography with substantial loss of 18. Nevertheless, the ease of preparation of 18 outweighted the poor yields of this strategy.
  • 15
    • 0006585604 scopus 로고
    • Eckstein, F., Ed.; Oxford University Press: Oxford
    • Conolly, B. A. In Oligonucletides and Analogues; Eckstein, F., Ed.; Oxford University Press: Oxford, 1991; pp 155-183.
    • (1991) Oligonucletides and Analogues , pp. 155-183
    • Conolly, B.A.1
  • 16
    • 0027240210 scopus 로고
    • +-form) 22, calcd 3155.0, found 3154.9; 26, calcd 3363.3, found 3364.4. Matrix conditions as described in the following: Pieles, U.; Zürcher, W.; Schär, M.; Moser, H. E. Nucleic Acids Res. 1993, 21, 3191.
    • (1993) Nucleic Acids Res. , vol.21 , pp. 3191
    • Pieles, U.1    Zürcher, W.2    Schär, M.3    Moser, H.E.4
  • 17
    • 85037493643 scopus 로고    scopus 로고
    • note
    • The HPLC trace of crude ODNs 22 and 26 showed that a substantial amount of byproducts was present - presumably not fully deprotected ODNs which arise from the capping step of the oligo synthesis. Acetic anhydride could acylate the trifluoroacetamide functions of the diaminoglucose residues and these acetamides might not be fully deprotected under standard deprotection conditions (concentrated ammonia at 55°C for 16 h). MALDI-ToF MS analysis of these byproducts was inconclusive.
  • 19
    • 0001193158 scopus 로고
    • 5-Aminoalkyl-and alkynyl-modified pyrimidine oligonucleotides also bind stronger to complementary DNA under low salt conditions and neutral pH than their unmodified counterparts, (a) Hashimoto, H.; Nelson, M. G.; Switzer, C. J. Am. Chem. Soc. 1993, 115, 7128.
    • (1993) J. Am. Chem. Soc. , vol.115 , pp. 7128
    • Hashimoto, H.1    Nelson, M.G.2    Switzer, C.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.