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Volumn 122, Issue 23, 2000, Pages 5638-5639

Minimalist, alanine-based, helical protein dimers bind to specific DNA sites [2]

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; DEOXYRIBONUCLEASE; DIMER; DNA; HISTIDINE; LEUCINE ZIPPER PROTEIN; UREA;

EID: 0034647207     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja993025a     Document Type: Letter
Times cited : (27)

References (30)
  • 14
    • 0343456014 scopus 로고    scopus 로고
    • note
    • We use the C/EBP zipper because in related experiments, we affix our proteins to solid support by diazotization through tyrosine; histidine may interfere with the diazotization reaction. See ref 11. The GCN4 zipper contains a histidine, whereas C/EBP does not.
  • 16
    • 0025091890 scopus 로고
    • We were concerned about the His tag; however, similar constructs were generated by Abate et al. who expressed the bZIP domains of Fos and Jun with 6-His tags at the N termini and found no interference with Jun-Fos interaction with the AP-1 site (see Abate, C.; Luk, D.; Gentz, R.; Rauscher, F. J., III; Curran, T. Proc. Natl. Acad. Sci. U.S.A. 1990, 87, 1032).
    • (1990) Proc. Natl. Acad. Sci. U.S.A. , vol.87 , pp. 1032
    • Abate, C.1    Luk, D.2    Gentz, R.3    Rauscher F.J. III4    Curran, T.5
  • 17
    • 0343891752 scopus 로고    scopus 로고
    • note
    • We note that amino acid 227 is arginine in both 4A and 11A; this is a cloning artifact, and this residue has no interaction with DNA (see refs 3-5).
  • 19
    • 0343891751 scopus 로고    scopus 로고
    • note
    • We note that 20 μM 18A does not appear to produce equally strong DNAse footprints on the 3′-radiolabeled DNA strand (Figure 3, lane 9) as on the 5′-radiolabeled strand (Figure 3 lane 19). This may be due to differences in the degree of protection that ISA affords to either strand in the major groove.
  • 21
    • 0343020421 scopus 로고    scopus 로고
    • note
    • Poor solubility of 18A precludes obtaining data at high protein concentrations. This limitation necessitated extrapolation of the binding curve.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.