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Volumn 7, Issue 18, 1997, Pages 2367-2372

Specific DNA binding peptide-derivatized solid support

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID COMPOSITION; ARTICLE; CIRCULAR DICHROISM; IN VITRO STUDY; PEPTIDE SYNTHESIS; PROTEIN DNA BINDING; PROTEIN IMMOBILIZATION; PROTEIN STRUCTURE; RECOGNITION;

EID: 0030821286     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00436-8     Document Type: Article
Times cited : (14)

References (27)
  • 18
    • 0342780408 scopus 로고    scopus 로고
    • note
    • 32P]-dATP. To dry DNA was added incubation buffer: 0.1 mg/mL bovine serum albumin (BSA, nuclease/protease-free, Sigma), 0.1 mg/mL tRNA (type XX, Sigma), 50 mM Tris, pH 8.0, 10 μg/mL gelatin, 1 mM EDTA, and 20 mM NaCl. Mixture was added to dimer-derivatized column, ≥1h, 4°C, and eluted with buffer containing increasing concentrations of NaCl. Radioactivity was assessed by scintillation counting.
  • 21
    • 0014062862 scopus 로고
    • Stoichiometric amounts of the tyrosine and histidine derivatives, N-chloroacetyl tyrosine and N-acetyl histidine, were reacted with the diazonium salt of m-nitroaniline and the time course followed under various buffers and pH conditions (Traylor, P. S.; Singer, S. J. Biochemistry 1967, 6, 881-887). Appropriately dilute conditions were chosen to mimic coupling conditions with gel (∼ 10 μmol free amine/mL drained gel).
    • (1967) Biochemistry , vol.6 , pp. 881-887
    • Traylor, P.S.1    Singer, S.J.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.