Convergence of the secretory pathways for cholera toxin and the filamentous phage, CTXφ

Science

Volumn 288, Issue 5464, 2000, Pages 333-335

  Davis, Brigid M ^a   Lawson, Elise H ^a   Sandkvist, Maria ^b   Ali, Afsar ^c   Sozhamannan, Shanmuga ^c   Waldor, Matthew K ^a  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b HOLLAND LABORATORY   (United States)

^c UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLERA TOXIN; OUTER MEMBRANE PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL OUTER MEMBRANE; BACTERIAL VIRULENCE; BACTERIOPHAGE; GENE TRANSFER; GENOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECRETION; TOXIN SYNTHESIS; VIBRIO CHOLERAE;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIOPHAGES; CHOLERA TOXIN; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; METALLOENDOPEPTIDASES; MUTAGENESIS, INSERTIONAL; PLASMIDS; TRANSDUCTION, GENETIC; VIBRIO CHOLERAE; VIRULENCE;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; UNIDENTIFIED BACTERIOPHAGE; VIBRIO CHOLERAE; VIBRIO PHAGE CTX;

EID: 0034646722     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5464.333     Document Type: Article

References (27)

1. B. B. Finlay and S. Falkow, Microbiol. Mol. Biol. Rev. 61, 136 (1997).
2. M. Russel, J. Mol. Biol. 279, 485 (1998).
3. P. J. Christie, J. Bacteriol. 179, 3085 (1997).
4. R. Binet, S. Letoffe, J. M. Ghigo, P. Delepelaire, C. Wandersman, Gene 192, 7 (1997).
5. K. J. Fullner and J. J. Mekalanos, Infect. Immun. 67, 1393 (1999).
6. M. K. Waldor and J. J. Mekalanos, Science 272, 1910 (1996).
7. D. K. Marciano, M. Russel, S. M. Simon, Science 284, 1516 (1999).
8. M. Russel, Trends Microbiol. 3, 223 (1995).
9. _, J. Mol. Biol. 231, 689 (1993).
10. We searched the V. cholerae database with the Institute for Genome Research's BLAST Search Engine for Unfinished Microbial Genomes (www.tigr.org/cgibin/BlastSearch/blast.cgi?).
11. M. Sandkvist et al., J. Bacteriol. 179, 6994 (1997).
12. J. B. Kaper, J. G. Morris Jr., M. M. Levine, Clin. Microbiol. Rev. 6, 48 (1995).
13. The targeting vector is described by A. Ali et al., Infect. Immun. 68, 1967 (2000).
14. B. M. Davis, E. H. Lawson, M. Sandkvist, M. K. Waldor, H. H. Kimsey, unpublished data.
15. G. D. Pearson, A. Woods, S. L. Chiang, J. J. Mekalanos, Proc. Natl. Acad. Sci. U.S.A. 90, 3750 (1993).
16. C. C. Hase and R. A. Finkelstein, J. Bacteriol. 173, 3311 (1991).
17. R, isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible plasmid pGZ1 19HE (24).
18. M. Judson and J. J. Mekalanos, unpublished data.
19. R.
20. pCTX-Kn is a more stable replicon than pCTX-Ap; this probably accounts for the consistently higher phage titers from strains containing pCTX-Kn. Transduction assays were performed as described in Table 1.
21. E. V. Koonin, FEBS Lett. 312, 3 (1992).
22. A. P. Pugsley and O. Francetic, Cell Mol. Life Sci. 54, 347 (1998).
23. M. Lindeberg, G. P. Salmond, A. Collmer, Mol. Microbiol. 20, 175 (1996).
24. M. Lessl et al., J. Bacteriol. 174, 2493 (1992).
25. M. Sandkvist, M. Bagdasarian, S. P. Howard, V. J. DiRita, EMBO J. 14, 1664 (1995).
26. M. Sandkvist, L. P. Hough, M. M. Bagdasarian, M. Bagdasarian, J. Bacteriol. 181, 3129 (1999).
27. We thank M. Russel, A. Camilli, A. L. Sonenshein, and Waldor lab colleagues for helpful suggestions and critical reading of the manuscript and A. Kane and the New England Medical Center GRASP Center for preparation of plates and media. S.S. thanks G. Morris and J. Johnson for research support. This work was supported by NIH grant AI-42347 to M.K.W. M.K.W. is a Pew Scholar in the Biomedical Sciences. M.S. was supported by funds from the American Red Cross. S.S. was supported by a University of Maryland Intramural Grant.