Enhanced cleavage of RNA mediated by an interaction between substrates and the arginine-rich domain of E. coli ribonuclease E

Journal of Molecular Biology

Volumn 301, Issue 2, 2000, Pages 257-264

  Kaberdin, Vladimir R ^a   Walsh, Andrew P ^b   Jakobsen, Thania ^a   McDowall, Kenneth J ^b   Von Gabain, Alexander ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

mRNA decay; RNA protein interaction

Indexed keywords

ARGININE; BACTERIAL RNA; MESSENGER RNA; OLIGORIBONUCLEOTIDE; RIBONUCLEASE; RIBONUCLEASE E; RNA 5S; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM MUTANT; ENZYME BINDING; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN RNA BINDING; RNA CLEAVAGE; RNA CONFORMATION; RNA STRUCTURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034636985     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3962     Document Type: Article

References (41)

1. The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli
2. Copurification of E. coli RNase E and PNPase: Evidence for a specific association between two enzymes important in RNA processing and degradation
3. Cloning and analysis of the entire Escherichia coli ams gene. ams is identical to hmp1 and encodes a 114 kDa protein that migrates as a 180 kDa protein
4. Correction: Cloning and analysis of the entire Escherichai coli ams gene
5. Degradation of mRNA in Escherichia coli: An old problem with some new twists
6. Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase
7. Surprises at the 3' end of prokaryotic RNA
8. RNase E: Still a wonderfully mysterious enzyme
9. Structural requirements for the processing of Escherichia coli: 5 S ribosomal RNA by RNase E in vitro
10. Specificity of Escherichia coli endoribonuclease RNase E: In vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site
11. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues
12. Poly(A)- and poly(U)-specific RNA 3' tail shortening by E. coli ribonuclease E
13. RNase E autoregulates its synthesis by controlling the degradation rate of its own messenger RNA in Escherichia coli - Unusual sensitivity of the rne transcript to RNase E activity
14. Real-time biospecific interaction analysis using surface plasmon resonance and a sensor chip technology
15. RNase E cleaves at multiple sites in bubble regions of RNA I stem loops yielding products that dissociate differentially from the enzyme
16. The endoribonucleolytic N-terminal half of Escherichia coli RNase E is evolutionarily conserved in Synechocystis sp. and other bacteria but not the C-terminal half, which is sufficient for degradosome assembly
17. The rate of processing and degradation of antisense RNAI regulates the replication of ColE1-type plasmids in vivo
18. Effects of nucleotide sequence on the specificity of rne-dependent and RNase E-mediated cleavages of RNA I encoded by the pBR322 plasmid
19. The C-terminal half of RNase E, which organizes the Escherichia coli degradosome, participates in mRNA degradation but not rRNA processing in vivo
20. The mechanisms of mRNA degradation in bacteria and their implication for stabilization of heterologous transcripts
21. Ribonuclease E is a 5'-end-dependent endonuclease
22. Modulation of the activity of RNase E in vitro by RNA sequences and secondary structures 5' to cleavage sites
23. T7 RNA polymerase does not interact with the 5'-phosphate of the initiating nucleotide
24. The N-terminal domain of the rne gene product has RNase E activity and is non-overlapping with the arginine-rich RNA-binding site
25. A + U content rather than a particular nucleotide order determines the specificity of RNase E cleavage
26. Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops
27. Genetic studies of cleavage-initiated mRNA decay and processing of ribosomal 9 S RNA show that the Escherichia coli ams and rne loci are the same
28. RNA processing and degradation by RNase K and RNase E
29. Proteins associated with RNase E in a multi-component ribonucleolytic complex
30. RNase E, an RNA processing enzyme from Escherichia coli
31. RNase E, an endoribonuclease, has a general role in the chemical decay of Escherichia coli mRNA: Evidence that rne and ams are the same genetic locus
32. A DEAD-box RNA helicase in the Escherichia coli RNA degradosome
33. Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro
34. Functional interaction of heat shock protein GroEL with an RNase E-like activity in Escherichia coli
35. The ribonucleoprotein substrate for a ribosomal RNA-processing nuclease
36. The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene
37. Evidence for an RNA-binding region in the Escherichia coli processing endoribonuclease RNase E
38. Processing enzyme ribonuclease E specifically cleaves RNA I. An inhibitor of primer formation in plasmid DNA synthesis
39. Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome
40. RNA degradation in Escherichia coli regulated by 3' adenylation and 5' phosphorylation