Sensory mechanism of oxygen sensor FixL from Rhizobium meliloti: Crystallographic, mutagenesis and resonance Raman spectroscopic studies

Journal of Molecular Biology

Volumn 301, Issue 2, 2000, Pages 415-431

  Miyatake, Hideyuki ^a   Mukai, Masahiro ^a   Park, Sam Yong ^a   Adachi, Shin Ichi ^a   Tamura, Koji ^a   Nakamura, Hiro ^a   Nakamura, Kayako ^a   Tsuchiya, Terumasa ^b   Iizuka, Tetsutaro ^a   Shiro, Yoshitsugu ^a  

^a RIKEN HARIMA INSTITUTE   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

Crystal structure; FixL; Heme protein; Oxygen sensor; Two component regulatory system

Indexed keywords

HEMOPROTEIN; OXYGEN; PROTEIN HISTIDINE KINASE; PROTOPORPHYRIN;

ARTICLE; CRYSTAL STRUCTURE; GENE EXPRESSION REGULATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; RAMAN SPECTROMETRY; SENSOR; SINORHIZOBIUM MELILOTI; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); RHIZOBIUM; SINORHIZOBIUM MELILOTI;

EID: 0034636982     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3954     Document Type: Article

References (41)

1. Present status of RIKEN beamline II (BL44B2) for structural biology
2. Recognition of target DNA and transcription activation by the CO-sensing transcriptional activator CooA
3. Assignment of the hyperfine-shifted 1H-NMR signals of the heme in the oxygen sensor FixL from Rhizobium meliloti
4. The CCP4 suite: Programs for protein crystallography
5. Neutron diffraction study of carbonmonoxymyoglobin
6. DM: An automated procedure for phase improvement by density modification
7. Crystal structure of yeast cytochrome c peroxidase refined at 1.7 Å resolution
8. Rhizobium: Plant signal exchange
9. PHASES-95: A program package for processing and analyzing diffraction data from macromolecules
10. Regulation of the kinase activity of heme protein FixL from the two-component system FixL/FixJ of Rhizobium meliloti
11. A haemoprotein with kinase activity encoded by the oxygen sensor of Rhizobium meliloti
12. Heme-based sensors, exemplified by the kinase FixL, are a new class of heme protein with distinctive ligand binding and autoxidation
13. Kinase activity of oxygen sensor FixL depends on the spin state of its heme iron
14. Structure of a biological oxygen sensor: A new mechanism for heme-driven signal transduction
15. New mechanistic insights from structural studies of the oxygen-sensing domain of Bradyrhizobium japonicum FixL
16. Mechanisms of autoxidation of the oxygen sensor FixL and Aplysia myoglobin: Implications for oxygen-binding heme proteins
17. Perturbation of the Fe-O2 bond by nearby residues in heme pocket: Observation of vFe-O2 Raman bands for oxymyoglobin mutants
18. Soluble guanylate cyclase form bovine lung: Activation with nitric oxide and carbon monoxide and spectral characterization of the ferrous and ferric states
19. Electron paramagnetic resonance spectral evidence for the formation of a pentacoordinate nitrosyl-heme complex on soluble guanylate cyclase
20. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
21. The oxygen sensor FixL of Rhizobium meliloti is a membrane protein containing four possible transmembrane segments
22. A program for photorealistic molecular graphics
23. Iron coordination structure of oxygen sensor FixL characterizied by Fe K-edge EXAFS and resonance Raman spectroscopy
24. Dynamic light-scattering and preliminary crystallographic studies of the sensor domain of the heam-based oxygen sensor of FixL from Rhizobium meliloti
25. The FixL protein of Rhizobium meliloti can be separated into a heme-binding oxygen-sensing domain and a functional C-terminal kinase domain
26. Identification of the hydrophobic amino acid residues required for heme assembly in the rhizobial oxygen sensor protein FixL
27. Protein folding and association; insights from the interfacial and thermodynamic properties of hydrocarbons
28. FixL, a haemoglobin that acts as an oxygen sensor: Signalling mechanism and structural basis of its homology with PAS domains
29. Metal chelate affinity chromatography, a new approach to protein fractionation
30. Structural basis for ligand discrimination and response initiation in the heme-based oxygen sensor FixL
31. The ethylene response mediator ETR1 from Arabidopsis forms a disulfide-linked dimer
32. Protein phosphorylation and regulation of adaptive responses in bacteria
33. The mode of attachment of the azide ion to sperm whale metmyoglobin
34. Nature of endogenous ligand binding to heme iron in oxygen sensor FixL
35. NMR structure of the histidine kinase domain of the E. coli osmosensor EnvZ
36. Structural implication of the heme-linked ionization of horseradiah peroxidase probed by the Fe-histidine streching Raman line
37. Generalized method of determining heavy-atom positions using the difference Patterson function
38. PAS domain S-boxes in Archaea, bacteria and sensors for oxygen and redox