Nature of endogenous ligand binding to heme iron in oxygen sensor FixL

Journal of the American Chemical Society

Volumn 118, Issue 39, 1996, Pages 9434-9435

  Tamura, Koji ^a   Nakamura, Hiro ^a   Tanaka, Yuji ^b   Oue, Soichi ^c   Tsukamoto, Koki ^d   Nomura, Masaharu ^e   Tsuchiya, Terumasa ^f   Adachi, Shin Ichi ^a   Takahashi, Satoshi ^a   Iizuka, Tetsutaro ^a   Shiro, Yoshitsugu ^a  

^a INST OF PHYS AND CHEMICAL RESEARCH   (Japan)

^b CHUO UNIVERSITY   (Japan)

^c GAKUSHUIN UNIVERSITY   (Japan)

^d NIHON UNIVERSITY   (Japan)

^e HIGH ENERGY ACCELERATOR RESEARCH ORGANIZATION KEK   (Japan)

^f TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

OXYGEN;

ARTICLE; DRUG DETERMINATION; ELECTRON SPIN RESONANCE; PLANT; PROTEIN BINDING; RAMAN SPECTROMETRY; TECHNIQUE;

EID: 0010266552     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9543457     Document Type: Article

References (26)

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7. 2 FixL* was prepared from E. coli strain JM109(DE3) after induction with IPTG as follows: The supernatant by ultracentrifugation of the lysed cells suspended in buffer A (20 mM Tris-HCl (pH 7.8), 50 mM NaCl, 5% (v/v) glycerol, 10 mM β-mercaptoethanol, and 1 mM PMSF) was applied to a DE52 (Whatman) column. FixL* was eluted with the buffer A containing 300 mM NaCl. It was applied onto a Ni-NTA agarose (QIAGEN) column and eluted with the gradient of imidazole (0-200 mM). Imidazole was removed by dialysis.
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18. 2. The optical absorption spectrum of the ferrous NO complex of FixLN at room temperature showed the six-coordination of the heme iron, with concomitant mixing of the five-coordination. However, the five-coordinated iron was predominant and the signal derived from the six-coordinated iron could hardly be seen in the frozen state at 20 K, as was manifested in the characteristic EPR feature.
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