A method for the large-scale cloning of nuclear proteins and nuclear targeting sequences on a functional basis

Analytical Biochemistry

Volumn 284, Issue 2, 2000, Pages 231-239

  Pichon, Bruno ^a   Mercan, Dany ^a   Pouillon, Valérie ^a   Christophe Hobertus, Christiane ^a   Christophe, Daniel ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

CLONE CELLS; CLONING; ENCODING (SYMBOLS); FLUORESCENCE;

BACTERIAL TRANSFORMATION; FUNCTIONAL BASIS; GREEN FLUORESCENT PROTEIN FUSION; GREEN FLUORESCENT PROTEIN MARKER; NUCLEAR LOCALIZATION SIGNAL; NUCLEAR PROTEINS; NUCLEAR TARGETING; TRANSFECTED CELLS;

PROTEINS;

COMPLEMENTARY DNA; DNA; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NUCLEAR PROTEIN;

ANIMAL CELL; ARTICLE; CELL SELECTION; CERCOPITHECIDAE; DNA LIBRARY; DNA TRANSFECTION; FLOW CYTOMETRY; FLUORESCENCE; GENETIC TRANSFECTION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

EID: 0034633268     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4674     Document Type: Article

References (27)

1. Exploring nuclear pore complex structure and function in molecular detail
2. Nucleocytoplasmic transport: Signals, mechanisms and regulation
3. Nuclear export receptors: From importin to exportin
4. The nucleus's revolving door
5. Importin β can mediate the nuclear import of an arginine-rich nuclear localization signal in the absence of importin α
6. A novel receptor-mediated nuclear protein import pathway
7. A novel nuclear import pathway for the transcription factor TFIIS
8. Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein
9. Capturing novel mouse genes encoding chromosomal and other nuclear proteins
10. Selection system for genes encoding nuclear-targeted proteins
11. Analysis of nuclear localization signals using a green fluorescent protein-fusion protein library
12. Critical residues of the homeodomain involved in contacting DNA bases also specify the nuclear accumulation of thyroid transcription factor-1
13. Study of TTF-1 gene expression in dog thyrocytes in primary culture
14. High efficiency gene transfer into mammalian cells
15. CAT Constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements
16. High efficiency transformation of E. coli by high voltage electroporation
17. The green fluorescent protein
18. Localization, trafficking and temperature-dependence of the Aequorea green fluorescent protein in cultured vertebrate cells
19. In vivo examination of membrane protein localization and degradation with green fluorescent protein
20. A fluorescent p53GFP fusion protein facilitates its detection in mammalian cells while retaining the properties of wild-type p53
21. Transient expression of proteins using COS cells
22. Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity
23. Identification and characterization of novel genes modulated in the thyroid of dogs treated with methimazole and propylthiouracil
24. The evolutionarily conserved Kruppel-associated box domain defines a subfamily of eukaryotic multifingered proteins
25. RNA-binding proteins as developmental regulators
26. DNA vector chemistry: The covalent attachment of signal peptides to plasmid DNA
27. Gene delivery: A single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus