Conversion of two-state to multi-state folding kinetics on fusion of two protein foldons

Journal of Molecular Biology

Volumn 302, Issue 1, 2000, Pages 219-233

  Inaba, Kenji ^a   Kobayashi, Naohiro ^a   Fersht, Alan R ^a  

^a MEDICAL RESEARCH COUNCIL   (United Kingdom)

Author keywords

CI2; Folding intermediate; Foldon; Protein design

Indexed keywords

CHYMOTRYPSIN INHIBITOR; HYBRID PROTEIN; POLYPEPTIDE;

ARTICLE; GENETIC ENGINEERING; KINETICS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STRUCTURE;

EID: 0034623013     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.4024     Document Type: Article

References (55)

1. Domains in folding of model proteins
2. Structure and dynamics of the acid-denatured molten globule state of α-lactalbumin: Two-dimensional NMR study
3. On-pathway versus off-pathway folding intermediates
4. Crystal structure of lysozyme by X-ray diffraction
5. Folding studies of immunoglobulin-like β-sandwich proteins suggest that they share a common folding pathway
6. Generation of a family of protein fragments for structure-folding studies. 1. Complementation of two fragments of chymotrypsin inhibitor-2 formed by cleavage at its unique methionine residue
7. Conformational pathway of the polypeptide chain of chymotrypsin inhibitor-2 growing from its N terminus in vitro. Parallels with the protein folding pathway
8. Principles of protein folding. A perspective from simple exact models
9. The folding mechanism of larger model proteins: Role of native structure
10. Genes in pieces: Were they ever together?
11. Rapid folding with and without populated intermediates in the homologous four-helix proteins Im7 and Im9
12. Protein folding and stability: The pathway of folding of barnase
13. Nucleation mechanisms in protein folding
14. Genes in species
15. Metal ion-dependence modulation of the dynamics of a designed protein
16. Structure and stability of monomeric 1 repressor: NMR evidence for two-state folding
17. The structure of the transition state for folding of chymotrypsin inhibitor 2 analyzed by protein engineering methods: Evidence for a nucleation-condensation mechanism for protein folding
18. How do small single-domain proteins fold?
19. Folding of chymotrypsin inhibitor 2; 1. Evidence for a two-state transition
20. Folding of chymotrypsin inhibitor 2; 2. Influence of proline isomerization on folding kinetics and thermodynamic characterization of the transition state folding
21. Effect of cavity-creating mutations in the hydrophobic core of chymotrypsin inhibitor 2
22. Evidence for a three-state model of protein folding from kinetic analysis of ubiquitin variants with altered core residues
23. Kinetic traps in lysozyme folding
24. Folding of a four-helix bundle: Studies of acyl-coenzyme A binding protein
25. A near-native state on the slow refolding pathway of hen lysozyme
26. Glutamine, alanine or glycine repeats inserted into the loop of a protein have minimal effects on stability and folding rates
27. Domain swapping in the sporulation response regulator Spo0A
28. Structure and stability of a second molten globule intermediate in the apomyoglobin folding pathway
29. Transient folding intermediates characterized by protein engineering
30. The folding of an enzyme; IV. Structure of an intermediate in the refolding of barnase analyzed by a protein engineering procedure
31. Crystal and molecular structure of the serine proteinase inhibitor CI-2 from barley seeds
32. Independent nucleation and heterogeneous assembly of structure during folding of equine lysozyme
33. Kinetic characterization of the chemotactic protein from Escherichia coli, Che Y-kinetic analysis of the inverse hydrophobic effect
34. Towards the complete structural characterization of a protein folding pathway: The structures of the denatured, transition and native states for the association/folding of two complementary fragments of cleaved chymotrypsin inhibitor 2. Direct evidence for a nucleation-condensation mechanism
35. Structure of the transition state for the folding/unfolding of the barley chymotrypsin inhibitor 2 and its implication for mechanisms of protein folding
36. Determination and analysis of urea and guanidine hydrochloride denaturation curves
37. Foldons, protein structural modules and exons
38. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions
39. A comparison of the folding kinetics and thermodynamics of two homologous fibronectin type HI modules
40. The folding of hen lysozyme involves partially structured intermediates and multiple pathways
41. Equilibrium stabiliy and submillisecond refolding of a designed single-chain Arc repressor
42. Extremely rapid folding in the absence of intermediates
43. The refolding of cispeptidylprolyl and trans-peptidylprolyl isomers of barstar
44. The folding pathway of the cell-cycle regulatory protein p13suc1: Clues for the mechanism of domain swapping
45. Crystal structure of a protein with an artificial exon-shuffling, module M4-substituted chimera hemoglobin βα, at 2.5 Å resolution
46. Incorporation of glutamine repeats makes protein oligomerize: Implications for neurodegenerative disease
47. Structure of bacteriophage T4 fibritin: A segmented coiled-coil and the role of the C-terminal domain
48. Thermodynamic and kinetic-analysis of the SH3 domain of spectrin shows a 2-state folding transition
49. A potential allosteric subsite generated by domain swapping in bovine seminal ribonuclease
50. 'Module' substitution in hemoglobin subunits
51. Three-state model for lysozyme folding: Triangular mechanism with an energetically trapped intermediate
52. Protein anatomy; functional roles of barnase module
53. Crystal structure of cyanovirin-N, a potent HIV-inactivating protein, shows unexpected domain swapping