Specification of Drosophila hematopoietic lineage by conserved transcription factors

Science

Volumn 288, Issue 5463, 2000, Pages 146-149

  Lebestky, Tim ^a   Chang, Ting ^b   Hartenstein, Volker ^a,b   Banerjee, Utpal ^a,b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b Dept Molec Cell Devmtl Biol   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR GATA 1; TRANSCRIPTION FACTOR GCM; TRANSCRIPTION FACTOR LZ; TRANSCRIPTION FACTOR RUNX1; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; DROSOPHILA; GENE EXPRESSION REGULATION; HEMATOPOIESIS; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE SENSITIVITY; TRANSCRIPTION REGULATION;

ARACHNIDA; DROSOPHILA; HEXAPODA; MAMMALIA;

EID: 0034616310     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.288.5463.146     Document Type: Article

References (37)

1. S. H. Orkin, Int. J. Dev. Biol. 42, 927 (1998).
2. M. H. Sieweke and T. Graf, Curr. Opin. Genet. Dev. 8, 545 (1998).
3. Y. Fujiwara, C. P. Browne, K. Cunniff, S. C. Goff, S. H. Orkin, Proc. Natl. Acad. Sci. U.S.A. 93, 12355 (1996).
4. F. Y. Tsai and S. H. Orkin, Blood 89, 3636 (1997).
5. C. N. Ting, M. C. Olson, K. P. Barton, J. M. Leiden, Nature 384, 474 (1996).
6. K. P. Rehorn, H. Thelen, A. M. Michelson, R. Reuter, Development 122, 4023 (1996).
7. T. H. Rabbitts, Nature 372, 143 (1994).
8. Q. Wang et al., Proc. Natl. Acad. Sci. U.S.A. 93, 3444 (1996); T. Okuda, J. van Deursen, S. W. Hiebert, C. Grosveld, J. R. Downing, Cell 84, 321 (1996); N. A. Speck et al., Cancer Res. 59, 1789s (1999); S. Bae and Y. Ito, Histol. Histopathol. 14, 1213 (1999).
9. Q. Wang et al., Proc. Natl. Acad. Sci. U.S.A. 93, 3444 (1996); T. Okuda, J. van Deursen, S. W. Hiebert, C. Grosveld, J. R. Downing, Cell 84, 321 (1996); N. A. Speck et al., Cancer Res. 59, 1789s (1999); S. Bae and Y. Ito, Histol. Histopathol. 14, 1213 (1999).
10. Q. Wang et al., Proc. Natl. Acad. Sci. U.S.A. 93, 3444 (1996); T. Okuda, J. van Deursen, S. W. Hiebert, C. Grosveld, J. R. Downing, Cell 84, 321 (1996); N. A. Speck et al., Cancer Res. 59, 1789s (1999); S. Bae and Y. Ito, Histol. Histopathol. 14, 1213 (1999).
11. Q. Wang et al., Proc. Natl. Acad. Sci. U.S.A. 93, 3444 (1996); T. Okuda, J. van Deursen, S. W. Hiebert, C. Grosveld, J. R. Downing, Cell 84, 321 (1996); N. A. Speck et al., Cancer Res. 59, 1789s (1999); S. Bae and Y. Ito, Histol. Histopathol. 14, 1213 (1999).
12. A. Daga, C. A. Karlovich, K. Dumstrei, U. Banerjee, Genes Dev. 10, 1194 (1996); G. V. Flores, A. Daga, H. R. Kalhor, U. Banerjee, Development 125, 3681 (1998).
13. A. Daga, C. A. Karlovich, K. Dumstrei, U. Banerjee, Genes Dev. 10, 1194 (1996); G. V. Flores, A. Daga, H. R. Kalhor, U. Banerjee, Development 125, 3681 (1998).
14. R. Bernardoni, B. Vivancos, A. Giangrande, Dev. Biol. 191, 118 (1997).
15. T. Hosoya, K. Takizawa, K. Nitta, Y. Hotta, Cell 82, 1025 (1995); B. W. Jones, R. D. Fetter, G. Tear, C. S. Goodman, Cell 82, 1013 (1995).
16. T. Hosoya, K. Takizawa, K. Nitta, Y. Hotta, Cell 82, 1025 (1995); B. W. Jones, R. D. Fetter, G. Tear, C. S. Goodman, Cell 82, 1013 (1995).
17. U. Tepass, L. I. Fessier, A. Aziz, V. Hartenstein, Development 120, 1829 (1994).
18. V. Riechmann, K. P. Rehorn, R. Reuter, M. Leptin, Development 125, 713 (1998).
19. B. Mathey-Prevot and N. Perrimon, Cell 92, 697 (1998); C. R. Dearolf, Biochim. Biophys. Acta 1377, M13 (1998).
20. B. Mathey-Prevot and N. Perrimon, Cell 92, 697 (1998); C. R. Dearolf, Biochim. Biophys. Acta 1377, M13 (1998).
21. T. M, Rizki, in Genetics and Biology of Drosophila, M. Ashburner and T. R. F. Wright, Eds. (Academic Press, London, 1978), vol. 2b, pp. 398-451.
22. T. Rizki, R. Rizki, E. Grell, Roux's Arch. Dev. Biol. 188, 91 (1980).
23. R. Shresta and E. Gatteff, Dev. Growth Differ. 24, 65 (1982).
24. A. E. Rugendorff, A. Younossi-Hartenstein, V. Hartenstein, Roux's Arch. Dev. Biol. 203, 266 (1993).
25. T. Rizki and R. Rizki, Genetics 97, s90 (1981).
26. T. Lebestky, T. Chang, V. Hartenstein, U. Banerjee, data not shown.
27. For further details, see Science Online (www. sciencemag.org/feature/data/1047010.shl).
28. ts1; Bc/+ embryos are raised at 29°C until third-instar larvae and are shifted to 25°C, crystal cells appear within 4.5 hours after the restoration of Lz function.
29. Antisense lz mRNA probe was made from the 3.5-kb lz cDNA (9). Rabbit antibody to Lz (9) was used at 1:50 dilution. Affinity-purified mouse antibody to Lz was used at 1:100 dilution. In situ hybridization and immunohistochemistry protocols were as described (12).
30. Lymph glands were dissected by cutting at 7/8 length along the larval body. Using fine tweezers, the inner tissue was inverted and lymph glands were separated from surrounding tissue. Lymph glands were fixed and stained using protocols derived from eye disc staining protocols as described (9).
31. Smears of circulating hemocytes from third-instar hemolymph were created by cutting larvae with fine tweezers and depositing the hemolymph on a glass slide. For immunolocalization, smears were fixed in 0.5% glutaraldehyde for 30 s, rinsed with PBT (PBS + 0.05% Triton) five times, incubated in PBT-N (PBS + 0.05% Triton + 10% normal goat serum) for 1 min, incubated in primary antibody overnight at 4°C, rinsed in PBT five times, washed in PBT-M for 1 min, incubated in secondary antibody for 2 hours at room temperature, rinsed five times in PBT, and mounted in Vectashield (Vector Laboratories) fluorescent mounting medium.
32. rA87 enhancer trap allele. Similarly, lz-lacZ refers to the lz-gal4; UAS-lacZ genotype.
33. J. Kim et al., Proc. Natl. Acad. Sci. U.S.A. 95, 12364 (1998); Y. Kanemura et al., FEBS Lett. 442, 151 (1999); Y. Akiyama, T. Hosoya, A. M. Poole, Y. Hotta, Proc. Natl. Acad. Sci. U.S.A. 93, 14912 (1996).
34. J. Kim et al., Proc. Natl. Acad. Sci. U.S.A. 95, 12364 (1998); Y. Kanemura et al., FEBS Lett. 442, 151 (1999); Y. Akiyama, T. Hosoya, A. M. Poole, Y. Hotta, Proc. Natl. Acad. Sci. U.S.A. 93, 14912 (1996).
35. J. Kim et al., Proc. Natl. Acad. Sci. U.S.A. 95, 12364 (1998); Y. Kanemura et al., FEBS Lett. 442, 151 (1999); Y. Akiyama, T. Hosoya, A. M. Poole, Y. Hotta, Proc. Natl. Acad. Sci. U.S.A. 93, 14912 (1996).
36. J. A. Hoffmann, F. C. Kafatos, C. A. Janeway, R. A. B. Ezekowitz, Science 284, 1318 (1999).
37. rA87, and Gcm antibody; C. Goodman and B. Jones for Gcm antibody; J. Pollock for the lz-gal4 stock; K. Matthews at the Bloomington Stock Center for many fly stocks; members of the Banerjee lab for critical review and comments on the manuscript; and E. Chan for his involvement in early stages of the project. Supported by U.S. Public Health Service National Research Service Award GM07185 (T.L.); NIH grant RO1EY08152, the McKnight Foundation Investigator Award, and the Margaret E. Early Medical Research Trust Award (U.B.); and NIH grant NS29367 (V.H.).